Analysis of the Brassica oleracea genome by the generation of B. campestris-oleracea chromosome addition lines: characterization by isozymes and rDNA genes

1987 ◽  
Vol 74 (6) ◽  
pp. 758-766 ◽  
Author(s):  
C. F. Quiros ◽  
O. Ochoa ◽  
S. F. Kianian ◽  
D. Douches
Keyword(s):  
Brassica Oleracea ◽  
Addition Lines ◽  
Rdna Genes ◽  
Chromosome Addition ◽  
Chromosome Addition Lines

scholarly journals Production and Characterization of Maize Chromosome 9 Radiation Hybrids Derived From an Oat-Maize Addition Line

Genetics ◽  
2000 ◽  
Vol 156 (1) ◽  
pp. 327-339 ◽  
Author(s):  
O Riera-Lizarazu ◽  
M I Vales ◽  
E V Ananiev ◽  
H W Rines ◽  
R L Phillips
Keyword(s):  
Radiation Hybrid ◽  
Chromosome Region ◽  
Chromosome 9 ◽  
Addition Line ◽  
Addition Lines ◽  
Organization Studies ◽  
Maize Chromosome ◽  
Chromosome Addition ◽  
Radiation Hybrids ◽  
Chromosome Addition Lines

Abstract In maize (Zea mays L., 2n = 2x = 20), map-based cloning and genome organization studies are often complicated because of the complexity of the genome. Maize chromosome addition lines of hexaploid cultivated oat (Avena sativa L., 2n = 6x = 42), where maize chromosomes can be individually manipulated, represent unique materials for maize genome analysis. Maize chromosome addition lines are particularly suitable for the dissection of a single maize chromosome using radiation because cultivated oat is an allohexaploid in which multiple copies of the oat basic genome provide buffering to chromosomal aberrations and other mutations. Irradiation (gamma rays at 30, 40, and 50 krad) of a monosomic maize chromosome 9 addition line produced maize chromosome 9 radiation hybrids (M9RHs)—oat lines possessing different fragments of maize chromosome 9 including intergenomic translocations and modified maize addition chromosomes with internal and terminal deletions. M9RHs with 1 to 10 radiation-induced breaks per chromosome were identified. We estimated that a panel of 100 informative M9RHs (with an average of 3 breaks per chromosome) would allow mapping at the 0.5- to 1.0-Mb level of resolution. Because mapping with maize chromosome addition lines and radiation hybrid derivatives involves assays for the presence or absence of a given marker, monomorphic markers can be quickly and efficiently mapped to a chromosome region. Radiation hybrid derivatives also represent sources of region-specific DNA for cloning of genes or DNA markers.

Transferability of wheat microsatellites to diploid Aegilops species and determination of chromosomal localizations of microsatellites in the S genome

Genome ◽  
2005 ◽  
Vol 48 (6) ◽  
pp. 959-970 ◽  
Author(s):  
I G Adonina ◽  
E A Salina ◽  
E G Pestsova ◽  
M S Röder
Keyword(s):  
Triticum Aestivum ◽  
Microsatellite Markers ◽  
Ssr Markers ◽  
Phylogenetic Relationships ◽  
Diploid Species ◽  
Aegilops Speltoides ◽  
Addition Lines ◽  
Chromosome Addition ◽  
Aegilops Longissima ◽  
Chromosome Addition Lines

Overall, 253 genomic wheat (Triticum aestivum) microsatellite markers were studied for their transferability to the diploid species Aegilops speltoides, Aegilops longissima, and Aegilops searsii, representing the S genome. In total, 88% of all the analyzed primer pairs of markers derived from the B genome of hexaploid wheat amplified DNA fragments in the genomes of the studied species. The transferability of simple sequence repeat (SSR) markers of the T. aestivum A and D genomes totaled 74%. Triticum aestivum – Ae. speltoides, T. aestivum – Ae. longissima, and T. aestivum – Ae. searsii chromosome addition lines allowed us to determine the chromosomal localizations of 103 microsatellite markers in the Aegilops genomes. The majority of them were localized to homoeologous chromosomes in the genome of Aegilops. Several instances of nonhomoeologous localization of T. aestivum SSR markers in the Aegilops genome were considered to be either amplification of other loci or putative translocations. The results of microsatellite analysis were used to study phylogenetic relationships among the 3 species of the Sitopsis section (Ae. speltoides, Ae. longissima, and Ae. searsii) and T. aestivum. The dendrogram obtained generally reflects the current views on phylogenetic relationships among these species.Key words: Triticum aestivum, Aegilops speltoides, Aegilops longissima, Aegilops searsii, microsatellite, SSR, chromosome addition lines, phylogeny.

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