The fermentation of hexose and pentose sugars by Candida shehatae and Pichia stipitis

1986 ◽  
Vol 23 (3-4) ◽  
pp. 228-233 ◽  
Author(s):  
J. C. du Preez ◽  
M. Bosch ◽  
B. A. Prior
Keyword(s):  
Pichia Stipitis ◽  
Candida Shehatae ◽  
Pentose Sugars

scholarly journals Metabolic Engineering of Saccharomyces cerevisiae for Conversion of d-Glucose to Xylitol and Other Five-Carbon Sugars and Sugar Alcohols

2007 ◽  
Vol 73 (17) ◽  
pp. 5471-5476 ◽  
Author(s):  
Mervi H. Toivari ◽  
Laura Ruohonen ◽  
Andrei N. Miasnikov ◽  
Peter Richard ◽  
Merja Penttilä
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Metabolic Engineering ◽  
Growth Medium ◽  
Fold Increase ◽  
Pichia Stipitis ◽  
Xylitol Dehydrogenase ◽  
Sugar Alcohols ◽  
Pentose Sugar ◽  
Encoding Gene ◽  
Pentose Sugars

ABSTRACT Recombinant Saccharomyces cerevisiae strains that produce the sugar alcohols xylitol and ribitol and the pentose sugar d-ribose from d-glucose in a single fermentation step are described. A transketolase-deficient S. cerevisiae strain accumulated d-xylulose 5-phosphate intracellularly and released ribitol and pentose sugars (d-ribose, d-ribulose, and d-xylulose) into the growth medium. Expression of the xylitol dehydrogenase-encoding gene XYL2 of Pichia stipitis in the transketolase-deficient strain resulted in an 8.5-fold enhancement of the total amount of the excreted sugar alcohols ribitol and xylitol. The additional introduction of the 2-deoxy-glucose 6-phosphate phosphatase-encoding gene DOG1 into the transketolase-deficient strain expressing the XYL2 gene resulted in a further 1.6-fold increase in ribitol production. Finally, deletion of the endogenous xylulokinase-encoding gene XKS1 was necessary to increase the amount of xylitol to 50% of the 5-carbon sugar alcohols excreted.

Hybridization of Pichia stipitis with its presumptive imperfect partner Candida shehatae

1987 ◽  
Vol 12 (3) ◽  
pp. 199-204 ◽  
Author(s):  
Abindra S. Gupthar ◽  
Helen M. Garnett
Keyword(s):  
Pichia Stipitis ◽  
Candida Shehatae

The electrophoretic banding pattern of the chromosomes of Pichia stipitis and Candida shehatae

1992 ◽  
Vol 22 (5) ◽  
pp. 429-431 ◽  
Author(s):  
V. Passoth ◽  
M. Hansen ◽  
U. Klinner ◽  
C. C. Emeis
Keyword(s):  
Banding Pattern ◽  
Pichia Stipitis ◽  
Candida Shehatae
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