Temperature profiles of growth and ethanol tolerance of the xylose-fermenting yeasts Candida shehatae and Pichia stipitis

1987 ◽  
Vol 25 (6) ◽  
pp. 521-525 ◽  
Author(s):  
J. C. du Preez ◽  
M. Bosch ◽  
B. A. Prior
1992 ◽  
Vol 38 (12) ◽  
pp. 1233-1237 ◽  
Author(s):  
Abindra S. Gupthar

A prototrophic strain of Saccharomyces cerevisiae CSIR Y190 MATa xyl−, resistant to high levels of ethanol, was hybridized with xylose-fermenting, auxotrophic mutants of Candida shehatae and Pichia stipitis through polyethylene glycol-induced protoplast fusion in an attempt to produce ethanol-tolerant, xylose-fermenting hybrids. Mononucleate fusants were obtained, but these dissociated into a mixture of parental-type segregants. Purified Candida- and Pichia-resembling segregants failed to acquire improved ethanol tolerance but expressed other novel properties of S. cerevisiae, suggesting that karyogamy was impaired after internuclear gene transfer. Key words: Pichia, Candida, Saccharomyces protoplast fusion.


1987 ◽  
Vol 12 (3) ◽  
pp. 199-204 ◽  
Author(s):  
Abindra S. Gupthar ◽  
Helen M. Garnett

1992 ◽  
Vol 22 (5) ◽  
pp. 429-431 ◽  
Author(s):  
V. Passoth ◽  
M. Hansen ◽  
U. Klinner ◽  
C. C. Emeis

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