Adaptation of Candida shehatae and Pichia stipitis to wood hydrolysates for increased ethanol production

1986 ◽  
Vol 25 (3) ◽  
Author(s):  
SaradR. Parekh ◽  
Shiyuan Yu ◽  
Morris Wayman
Keyword(s):  
Ethanol Production ◽  
Pichia Stipitis ◽  
Candida Shehatae

Ethanol Production from Corn Stover Using Soaking Pretreatment

2010 ◽  
Vol 171-172 ◽  
pp. 261-265
Author(s):  
Zhuang Zuo ◽  
Xiu Shan Yang
Keyword(s):  
Ethanol Production ◽  
Corn Stover ◽  
Simultaneous Saccharification And Fermentation ◽  
Pichia Stipitis ◽  
Liquid Ratio ◽  
Mild Conditions ◽  
Whole Process ◽  
Conversion Rates ◽  
Mild Temperature ◽  
Simultaneous Saccharification

Corn stover was pretreated using different soaking conditions at mild temperature. Among the tested conditions, the best was 1% NaOH+8% NH4OH，50°C，48 h, Solid-to-liquid ratio 1:10. The results showed that soaking pretreatment achieved 63.6% delignification, retained the xylan and glucan. After enzymatic hydrolysis, conversion rates of xylan and glucan were 70.9% and 78.5%, respectively. The pretreated filtration re-soaking cause 52.7% xylan and 65.0% glucan conversion. NaOH+NH4OH treatment can be performed under mild conditions, gives a good buffering effect, low carbohydates degradation and extensive removal of lignin. Additionally, simultaneous saccharification and fermentation was conducted with pretreated corn stover to assess the ethanol production. For the whole process, 0.15g ethanol /g corn stover was achieved using Saccharomyces cerevisiae Y5, and 0.19g ethanol /g corn stover when using Pichia stipitis.

Enhancing ethanol production from cellulosic sugars using Scheffersomyces (Pichia) stipitis

2016 ◽  
Vol 39 (7) ◽  
pp. 1023-1032 ◽  
Author(s):  
C. C. Okonkwo ◽  
M. M. Azam ◽  
T. C. Ezeji ◽  
N. Qureshi
Keyword(s):  
Ethanol Production ◽  
Pichia Stipitis ◽  
Cellulosic Sugars

scholarly journals Ethanol Production from Nondetoxified Dilute-Acid Lignocellulosic Hydrolysate by Cocultures of Saccharomyces cerevisiae Y5 and Pichia stipitis CBS6054

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Ping Wan ◽  
Dongmei Zhai ◽  
Zhen Wang ◽  
Xiushan Yang ◽  
Shen Tian
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Production ◽  
Ethanol Concentration ◽  
Ethanol Yield ◽  
Synthetic Medium ◽  
Pichia Stipitis ◽  
Dilute Acid ◽  
Acid Hydrolysate ◽  
Issatchenkia Orientalis ◽  
Final Ethanol Concentration

Saccharomyces cerevisiae Y5 (CGMCC no. 2660) and Issatchenkia orientalis Y4 (CGMCC no. 2159) were combined individually with Pichia stipitis CBS6054 to establish the cocultures of Y5 + CBS6054 and Y4 + CBS6054. The coculture Y5 + CBS6054 effectively metabolized furfural and HMF and converted xylose and glucose mixture to ethanol with ethanol concentration of 16.6 g/L and ethanol yield of 0.46 g ethanol/g sugar, corresponding to 91.2% of the maximal theoretical value in synthetic medium. Accordingly, the nondetoxified dilute-acid hydrolysate was used to produce ethanol by co-culture Y5 + CBS6054. The co-culture consumed glucose along with furfural and HMF completely in 12 h, and all xylose within 96 h, resulting in a final ethanol concentration of 27.4 g/L and ethanol yield of 0.43 g ethanol/g sugar, corresponding to 85.1% of the maximal theoretical value. The results indicated that the co-culture of Y5 + CBS6054 was a satisfying combination for ethanol production from non-detoxified dilute-acid lignocellulosic hydrolysates. This co-culture showed a promising prospect for industrial application.

Influence of metal addition on ethanol production with Pichia stipitis ATCC 58784

2008 ◽  
Vol 36 (4) ◽  
pp. 491-497 ◽  
Author(s):  
Yuan Li ◽  
Zhenya Zhang ◽  
Zhongfang Lei ◽  
Yingnan Yang ◽  
Motoo Utsumi ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Pichia Stipitis ◽  
Metal Addition

Bioconversion of De-Oiled Rice Bran (DORB) Hemicellulosic Hydrolysate into Ethanol by Pichia stipitis NCM3499 under Optimized Conditions

2009 ◽  
Vol 5 (1) ◽  
Author(s):  
Anuj Kumar Chandel ◽  
Mangamoori Lakshmi Narasu ◽  
Ravinder Rudravaram ◽  
Ravindra Pogaku ◽  
Linga Venkateswar Rao
Keyword(s):  
Ethanol Production ◽  
Rice Bran ◽  
Process Parameters ◽  
Reducing Sugars ◽  
Carbon Sources ◽  
Pichia Stipitis ◽  
Medium Temperature ◽  
Carbon Substrates ◽  
Total Reducing Sugars ◽  
Optimized Conditions

In the present paper, parametric optimization studies were performed to determine the most influential range of process parameters for maximum ethanol production from Pichia stipitis NCIM3499 by one-at-a-time method under submerged fermentation conditions. Various parameters, such as agitation, pH of medium, temperature and different carbon sources, etc., were taken into consideration. The optimum values of these process parameters were as follows: agitation (200 rpm), pH (5.5), temperature (30°C), and semi-aerobic conditions (150 ml fermentation media in 250 ml Erlenmeyer flask). Among the different carbon substrates tested, glucose and xylose (20±0.21 g/l) showed same ethanol production (8.8±0.35 g/l) with a yield of 0.44±0.11g/g sugar utilized. These process parameters were applied for the ethanol production from de-oiled rice bran (DORB), the left over residue after extraction of oil. It was thermochemically saccharified using sulfuric acid (0.5-5.5% v/v) at 120°C for a fixed residence time of 1h. A maximum of 38.50±0.45 g/l total reducing sugars was obtained at 3.5% v/v H2SO4. This sugar syrup was detoxified by overliming with calcium hydroxide and subsequently fermented with P. stipitis NCIM3499 under the optimized conditions. After 72 h of incubation, DORB hydrolysate (33.50±0.44g/l total reducing sugars) showed maximum ethanol production (12.47±0.26g/l) with the yield 0.42±0.021g/g and fermentation efficiency of 81.74±0.55%. To the best of our knowledge, this is the first report on ethanol production from de-oiled rice bran using P. stipitis NCIM3499.

Sign in / Sign up

Export Citation Format

Share Document