Genetic analysis of Aspergillus niger: Isolation of chlorate resistance mutants, their use in mitotic mapping and evidence for an eighth linkage group

1990 ◽  
Vol 221 (3) ◽  
pp. 453-458 ◽  
Author(s):  
Alfons J. M. Debets ◽  
Klaas Swart ◽  
Cees J. Bos
Keyword(s):  
Aspergillus Niger ◽  
Linkage Group ◽  
Genetic Analysis ◽  
Chlorate Resistance ◽  
Resistance Mutants

scholarly journals Genetic analysis by means of the parasexual cycle in Aspergillus niger

1967 ◽  
Vol 10 (1) ◽  
pp. 45-61 ◽  
Author(s):  
Pol Lhoas
Keyword(s):  
Aspergillus Niger ◽  
Linkage Group ◽  
Genetic Analysis ◽  
Sexual Cycle ◽  
Crossing Over ◽  
Linkage Groups ◽  
Parasexual Cycle ◽  
Sexual Species ◽  
Data Support ◽  
Almost All

An investigation of mitotic segregation and recombination in A. niger gave the following results:1. Thirty-one non-allelic markers have been assigned to six linkage groups (containing 11, 9, 6, 3, 1 and 1 markers respectively) by the analysis of haploid mitotic segregants from synthesized diploids.2. The sequence of nine markers in one linkage group was determined and some of the map intervals were estimated by the analysis of haploids, recombinants for linked markers.3. Almost all the haploid segregants were obtained on medium supplemented with the aminoacid analogue, p-fluoro-phenylalanine, the action of which is interpreted as an induction of chromosome losses.4. The rates of mitotic crossing-over and haploidization are much higher than in the sexual species A. nidulans and the data support Pontecorvo's (1958) suggestion that the parasexual cycle can be a substantial alternative to the sexual cycle.

Mitotic mapping in linkage group V of Aspergillus niger based on selection of auxotrophic recombinants by Novozym enrichment

1989 ◽  
Vol 35 (11) ◽  
pp. 982-988 ◽  
Author(s):  
Alfons J. M. Debets ◽  
Klaas Swart ◽  
Cees J. Bos
Keyword(s):  
Aspergillus Niger ◽  
Linkage Group ◽  
Genetic Analysis ◽  
Chromosome Mapping ◽  
Crossing Over ◽  
Diploid Strain ◽  
Group V ◽  
Clonal Distribution ◽  
Homozygous Diploid ◽  
Selection Of

This paper describes a procedure which allows the quantitative selection of auxotrophs of the fungus Aspergillus niger by enzymatic killing of immobilized germinating prototrophic conidiospores. We have applied this procedure to linkage analysis on the basis of mitotic crossing-over in this fungus. Starting with a heterozygous diploid strain, we could select auxotrophic homozygous diploid recombinants quantitatively. We estimated the frequency of crossing-over after correction for clonal distribution of recombinants, and localized four auxotrophic markers as well as the centromere on chromosome V of this fungus. The Novozym enrichment procedure proved to be useful in genetic analysis and for the construction of recombinant genotypes in the case of closely linked auxotrophic markers. The detemination of gene order and the estimation of distances on the basis of benomyl-induced recombinant haploid segregants may lead to incorrect conclusions. Genetic analysis on the basis of homozygous recombinants, however, can provide reliable estimates of map distances.Key words: Aspergillus niger, chromosome mapping, mitotic crossing-over, Novozym enrichment, auxotrophic recombinants.

Linkage group analysis in Aspergillus niger

1993 ◽  
Vol 38 (6) ◽  
pp. 742-745 ◽  
Author(s):  
C. J. Bos ◽  
S. M. Slakhorst ◽  
A. J. M. Debets ◽  
K. Swart
Keyword(s):  
Aspergillus Niger ◽  
Linkage Group ◽  
Group Analysis

scholarly journals Centromere-Linkage Analysis and Consolidation of the Zebrafish Genetic Map

Genetics ◽  
1996 ◽  
Vol 142 (4) ◽  
pp. 1277-1288
Author(s):  
Stephen L Johnson ◽  
Michael A Gates ◽  
Michele Johnson ◽  
William S Talbot ◽  
Sally Horne ◽  
...  
Keyword(s):  
Linkage Group ◽  
Linkage Analysis ◽  
Genetic Analysis ◽  
Linkage Map ◽  
Rapid Method ◽  
Genetic Map ◽  
Dna Polymorphisms ◽  
Linkage Groups

Abstract The ease of isolating mutations in zebrafish will contribute to an understanding of a variety of processes common to all vertebrates. To facilitate genetic analysis of such mutations, we have identified DNA polymorphisms closely linked to each of the 25 centromeres of zebrafish, placed centromeres on the linkage map, increased the number of mapped PCR-based markers to 652, and consolidated the number of linkage groups to the number of chromosomes. This work makes possible centromere-linkage analysis, a novel, rapid method to assign mutations to a specific linkage group using half-tetrads.

scholarly journals Transcriptomic and molecular genetic analysis of the cell wall salvage response of Aspergillus niger to the absence of galactofuranose synthesis

2016 ◽  
Vol 18 (9) ◽  
pp. 1268-1284 ◽  
Author(s):  
Joohae Park ◽  
Mark Hulsman ◽  
Mark Arentshorst ◽  
Matthijs Breeman ◽  
Ebru Alazi ◽  
...  
Keyword(s):  
Cell Wall ◽  
Aspergillus Niger ◽  
Genetic Analysis ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis

ISOLATION AND GENETIC ANALYSIS OF MMS-SENSITIVE MUS MUTANTS OF NEUROSPORA

1980 ◽  
Vol 22 (4) ◽  
pp. 535-552 ◽  
Author(s):  
E. Käfer ◽  
E. Perlmutter
Keyword(s):  
Dna Repair ◽  
Linkage Group ◽  
Genetic Analysis ◽  
Double Mutant ◽  
Excision Repair ◽  
Methylmethane Sulfonate ◽  
New Genes ◽  
Mutant Strains ◽  
Error Prone Repair ◽  
Isogenic Strains

With the aim of obtaining mutants that affect DNA repair or recombination, mutants sensitive to methylmethane sulfonate (MMS) have been isolated in the ascomycete Neurospora crassa. Seven of these mutants were backcrossed repeatedly to produce isogenic strains for measurements of relative mutagen sensitivities and for analysis of recombination frequencies. The new mus (mutagen sensitives) were compared to four previously known radiation-sensitive mutants which were shown to be cross-sensitive to MMS. Tests for allelism assigned the mus mutants to five new genes, mus-7 to mus-11, each mapping in a different linkage group. In homozygous crosses all mutants were sterile, except the two alleles of gene mus-10 which occasionally produced some viable ascospores. Complementation tests on MMS-media identified double mutant strains from many intercrosses. Such strains can be used for analysis of interactions between mutant alleles from different genes and of possible epistatic groupings for repair-deficient mutants in Neurospora. Four of these double mutant strains, all containing mus-8 and previously known mutants, were checked for survival on MMS media and their sensitivities were compared to those of their parental single mutant strains. Results indicate that mus-8 may be epistatic to uvs-2 which is deficient in excision repair, but not to mutants like uvs-3 that appear to be deficient in error-prone repair.

scholarly journals Genetic Analysis of Adult-Specific Surface Antigenic Differences Between Varieties of the Nematode Caenorhabditis elegans

Genetics ◽  
1987 ◽  
Vol 117 (3) ◽  
pp. 467-476
Author(s):  
Samuel M Politz ◽  
Karl J Chin ◽  
Daniel L Herman
Keyword(s):  
Caenorhabditis Elegans ◽  
Linkage Group ◽  
Genetic Analysis ◽  
Surface Antigen ◽  
Single Gene ◽  
Surface Antigens ◽  
Nematode Caenorhabditis Elegans ◽  
Specific Antibodies ◽  
C Elegans ◽  
Stage Specificity

ABSTRACT We have studied developmental stage-specificity and genetic specification of surface antigens in the nematode Caenorhabditis elegans. Rabbit antisera directed against the adult C. elegans cuticle were used in conjunction with antiserum adsorption experiments to obtain antibody reagents with specificity for the adult surface. Adult-specific antibodies were used to identify several varietal strains of C. elegans that display antigen-negative phenotypes as adults. Genetic mapping results using the surface antigen phenotype as a marker indicated that a single gene (designated srf-1) or cluster of genes on linkage group II determines the adult surface antigen phenotype.

PARASEXUAL GENETIC ANALYSIS OF CELL PROPORTIONING MUTANTS OF DICTYOSTELIUM DISCOIDEUM

Genetics ◽  
1981 ◽  
Vol 99 (2) ◽  
pp. 183-196
Author(s):  
James H Morrissey ◽  
William F Loomis
Keyword(s):  
Cell Differentiation ◽  
Linkage Group ◽  
Genetic Analysis ◽  
Dictyostelium Discoideum ◽  
Double Mutant ◽  
Complementation Group ◽  
Stalk Cell ◽  
Recessive Mutation ◽  
Group Vi ◽  
Recessive Mutations

ABSTRACT Eight independently isolated mutants of Dictyostelium discoideum that differentiate exclusively into stalk cells make up one complementation group and carry single recessive mutations at the stalky locus, stkA, located on linkage group II. KY19, a previously described strain that differentiates into spores, but not stalk cells, was found to possess a recessive mutation defining the stalkless locus, stlA, located on linkage group VI. An analysis of the properties of these mutants, together with the phenotype of a haploid double mutant carrying stkA and stlA indicates that stlA results in poorly organized stalk tubes and incomplete stalk cell differentiation, while stkA causes all of the cells to differentiate into stalk cells, even when not enclosed in the stalk tube. The significance of these results is discussed in relation to current theories of pattern formation in D. discoideum.

Genetic analysis of Aspergillus niger mutants defective in benzoate-4-hydroxylase function

1991 ◽  
Vol 19 (4) ◽  
pp. 261-264 ◽  
Author(s):  
J. G. Boschloo ◽  
E. Moonen ◽  
R. F. M. van Gorcom ◽  
H. F. M. Hermes ◽  
C. J. Bos
Keyword(s):  
Aspergillus Niger ◽  
Genetic Analysis
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