Linkage group analysis in Aspergillus niger

1993 ◽  
Vol 38 (6) ◽  
pp. 742-745 ◽  
Author(s):  
C. J. Bos ◽  
S. M. Slakhorst ◽  
A. J. M. Debets ◽  
K. Swart
Keyword(s):  
Aspergillus Niger ◽  
Linkage Group ◽  
Group Analysis

scholarly journals NEW MUTATIONS AND A 7-CHROMOSOME LINKAGE MAP OF SCHIZOPHYLLUM COMMUNE

Genetics ◽  
1977 ◽  
Vol 85 (3) ◽  
pp. 417-425
Author(s):  
Carl Frankel ◽  
Albert H Ellingboe
Keyword(s):  
Linkage Group ◽  
Linkage Map ◽  
Group Analysis ◽  
Schizophyllum Commune ◽  
Somatic Recombination ◽  
Linkage Relationships ◽  
New Mutations

ABSTRACT Forty-eight useful new mutations of S. commune were obtained by mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine. Their requirements and meiotic linkage relationships to each other and previously mapped areas were investigated. Several of these new mutations were incorporated into diploid strains so that the diploids contained at least one marker on every linkage group. Analysis of somatic recombination in these diploids indicated that each meiotic linkage group corresponded to an independent chromosome.

scholarly journals Linkage Group Analysis of Malathion Resistance in the Housefly

1982 ◽  
Vol 4 (3) ◽  
pp. 289-299 ◽  
Author(s):  
Toshio SHONO ◽  
Masahiro HORIO ◽  
Masuhisa TSUKAMOTO
Keyword(s):  
Linkage Group ◽  
Group Analysis

scholarly journals Genetic analysis by means of the parasexual cycle in Aspergillus niger

1967 ◽  
Vol 10 (1) ◽  
pp. 45-61 ◽  
Author(s):  
Pol Lhoas
Keyword(s):  
Aspergillus Niger ◽  
Linkage Group ◽  
Genetic Analysis ◽  
Sexual Cycle ◽  
Crossing Over ◽  
Linkage Groups ◽  
Parasexual Cycle ◽  
Sexual Species ◽  
Data Support ◽  
Almost All

An investigation of mitotic segregation and recombination in A. niger gave the following results:1. Thirty-one non-allelic markers have been assigned to six linkage groups (containing 11, 9, 6, 3, 1 and 1 markers respectively) by the analysis of haploid mitotic segregants from synthesized diploids.2. The sequence of nine markers in one linkage group was determined and some of the map intervals were estimated by the analysis of haploids, recombinants for linked markers.3. Almost all the haploid segregants were obtained on medium supplemented with the aminoacid analogue, p-fluoro-phenylalanine, the action of which is interpreted as an induction of chromosome losses.4. The rates of mitotic crossing-over and haploidization are much higher than in the sexual species A. nidulans and the data support Pontecorvo's (1958) suggestion that the parasexual cycle can be a substantial alternative to the sexual cycle.

Mitotic mapping in linkage group V of Aspergillus niger based on selection of auxotrophic recombinants by Novozym enrichment

1989 ◽  
Vol 35 (11) ◽  
pp. 982-988 ◽  
Author(s):  
Alfons J. M. Debets ◽  
Klaas Swart ◽  
Cees J. Bos
Keyword(s):  
Aspergillus Niger ◽  
Linkage Group ◽  
Genetic Analysis ◽  
Chromosome Mapping ◽  
Crossing Over ◽  
Diploid Strain ◽  
Group V ◽  
Clonal Distribution ◽  
Homozygous Diploid ◽  
Selection Of

This paper describes a procedure which allows the quantitative selection of auxotrophs of the fungus Aspergillus niger by enzymatic killing of immobilized germinating prototrophic conidiospores. We have applied this procedure to linkage analysis on the basis of mitotic crossing-over in this fungus. Starting with a heterozygous diploid strain, we could select auxotrophic homozygous diploid recombinants quantitatively. We estimated the frequency of crossing-over after correction for clonal distribution of recombinants, and localized four auxotrophic markers as well as the centromere on chromosome V of this fungus. The Novozym enrichment procedure proved to be useful in genetic analysis and for the construction of recombinant genotypes in the case of closely linked auxotrophic markers. The detemination of gene order and the estimation of distances on the basis of benomyl-induced recombinant haploid segregants may lead to incorrect conclusions. Genetic analysis on the basis of homozygous recombinants, however, can provide reliable estimates of map distances.Key words: Aspergillus niger, chromosome mapping, mitotic crossing-over, Novozym enrichment, auxotrophic recombinants.

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