Genetic analysis of Aspergillus niger mutants defective in benzoate-4-hydroxylase function

1991 ◽  
Vol 19 (4) ◽  
pp. 261-264 ◽  
Author(s):  
J. G. Boschloo ◽  
E. Moonen ◽  
R. F. M. van Gorcom ◽  
H. F. M. Hermes ◽  
C. J. Bos
2016 ◽  
Vol 18 (9) ◽  
pp. 1268-1284 ◽  
Author(s):  
Joohae Park ◽  
Mark Hulsman ◽  
Mark Arentshorst ◽  
Matthijs Breeman ◽  
Ebru Alazi ◽  
...  

2001 ◽  
Vol 52 (2-3) ◽  
pp. 335-343 ◽  
Author(s):  
K. Swart ◽  
A.J.M. Debets ◽  
M. Slakhorst ◽  
E.F. Holub ◽  
R.F. Hoekstra ◽  
...  

1967 ◽  
Vol 10 (1) ◽  
pp. 45-61 ◽  
Author(s):  
Pol Lhoas

An investigation of mitotic segregation and recombination in A. niger gave the following results:1. Thirty-one non-allelic markers have been assigned to six linkage groups (containing 11, 9, 6, 3, 1 and 1 markers respectively) by the analysis of haploid mitotic segregants from synthesized diploids.2. The sequence of nine markers in one linkage group was determined and some of the map intervals were estimated by the analysis of haploids, recombinants for linked markers.3. Almost all the haploid segregants were obtained on medium supplemented with the aminoacid analogue, p-fluoro-phenylalanine, the action of which is interpreted as an induction of chromosome losses.4. The rates of mitotic crossing-over and haploidization are much higher than in the sexual species A. nidulans and the data support Pontecorvo's (1958) suggestion that the parasexual cycle can be a substantial alternative to the sexual cycle.


2013 ◽  
Vol 63 (2) ◽  
pp. 179-186 ◽  
Author(s):  
Ruiko HASHIMOTO ◽  
Katsuyoshi ASANO ◽  
Tadaaki TOKASHIKI ◽  
Yoshiki ONJI ◽  
Mina HIROSE-YASUMOTO ◽  
...  

1998 ◽  
Vol 33 (2) ◽  
pp. 145-150 ◽  
Author(s):  
A. J. G. Engels ◽  
E. F. Holub ◽  
K. Swart ◽  
M. A. De Waard

1988 ◽  
Vol 14 (5) ◽  
pp. 437-443 ◽  
Author(s):  
C. J. Bos ◽  
A. J. M. Debets ◽  
K. Swart ◽  
A. Huybers ◽  
G. Kobus ◽  
...  

1990 ◽  
Vol 34 (2) ◽  
pp. 225-228 ◽  
Author(s):  
J. G. Boschloo ◽  
A. Paffen ◽  
T. Koot ◽  
W. J. J. van den Tweel ◽  
R. F. M. van Gorcom ◽  
...  

1989 ◽  
Vol 35 (11) ◽  
pp. 982-988 ◽  
Author(s):  
Alfons J. M. Debets ◽  
Klaas Swart ◽  
Cees J. Bos

This paper describes a procedure which allows the quantitative selection of auxotrophs of the fungus Aspergillus niger by enzymatic killing of immobilized germinating prototrophic conidiospores. We have applied this procedure to linkage analysis on the basis of mitotic crossing-over in this fungus. Starting with a heterozygous diploid strain, we could select auxotrophic homozygous diploid recombinants quantitatively. We estimated the frequency of crossing-over after correction for clonal distribution of recombinants, and localized four auxotrophic markers as well as the centromere on chromosome V of this fungus. The Novozym enrichment procedure proved to be useful in genetic analysis and for the construction of recombinant genotypes in the case of closely linked auxotrophic markers. The detemination of gene order and the estimation of distances on the basis of benomyl-induced recombinant haploid segregants may lead to incorrect conclusions. Genetic analysis on the basis of homozygous recombinants, however, can provide reliable estimates of map distances.Key words: Aspergillus niger, chromosome mapping, mitotic crossing-over, Novozym enrichment, auxotrophic recombinants.


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