?-Tubulin in tobacco pollen tubes: association with generative cell and vegetative microtubules

1994 ◽  
Vol 7 (4) ◽  
Author(s):  
BarryA. Palevitz ◽  
Bo Liu ◽  
HarishC. Joshi
Keyword(s):  
Generative Cell ◽  
Pollen Tubes ◽  
Tobacco Pollen

DIACYLGLYCEROL KINASE 5 regulates polar tip growth of tobacco pollen tubes

2021 ◽  
Author(s):  
Patricia Scholz ◽  
Přemysl Pejchar ◽  
Max Fernkorn ◽  
Eliška Škrabálková ◽  
Roman Pleskot ◽  
...  
Keyword(s):  
Tip Growth ◽  
Diacylglycerol Kinase ◽  
Pollen Tubes ◽  
Tobacco Pollen ◽  
Polar Tip Growth

Morphology of Cell Injury: An Approach to the EDIT Programme by the Use of Tobacco Pollen Tubes

2002 ◽  
Vol 30 (3) ◽  
pp. 323-329 ◽  
Author(s):  
Udo Kristen ◽  
Natalie Bischoff ◽  
Saskia Lisboa ◽  
Enno Schirmer ◽  
Sören Witt ◽  
...  
Keyword(s):  
Tip Growth ◽  
Cell Injury ◽  
Cytosolic Calcium ◽  
Pollen Tubes ◽  
In Vitro Cytotoxicity ◽  
Toxic Compounds ◽  
In Vitro System ◽  
Tobacco Pollen ◽  
Different Types

Tobacco pollen tubes were used as a standard in vitro system to investigate cell growth aberrations caused by some of the Multicentre Evaluation of In Vitro Cytotoxicity (MEIC) programme chemicals and other toxic compounds. Changes in cytoskeletal pattern were observed in the tube cells by using tubu-lin immunofluorescence and rhodamin–phalloidin fluorescence for the localisation of microtubules and actin filaments, respectively. Four different types of cell malformation were found: screw-like growth, isodiametric tip swelling, hook formation, and pollen grain enlargement. We suggest that these malformations resulted from an interference by the chemicals with the cytosolic calcium gradient which controls tip growth and the orientation of the pollen tube. The results may contribute to a general understanding of toxicity-based cell malformations.

scholarly journals Prunus necrotic ringspot virus Early Invasion and Its Effects on Apricot Pollen Grain Performance

2007 ◽  
Vol 97 (8) ◽  
pp. 892-899 ◽  
Author(s):  
Khalid Amari ◽  
Lorenzo Burgos ◽  
Vicente Pallas ◽  
María Amelia Sanchez-Pina
Keyword(s):  
Developmental Stages ◽  
Generative Cell ◽  
Pollen Grains ◽  
Growth Zone ◽  
Pollen Tubes ◽  
Climatic Conditions ◽  
Pollen Grain ◽  
Ringspot Virus ◽  
Prunus Necrotic Ringspot Virus

The route of infection and the pattern of distribution of Prunus necrotic ringspot virus (PNRSV) in apricot pollen were studied. PNRSV was detected both within and on the surface of infected pollen grains. The virus invaded pollen during its early developmental stages, being detected in pollen mother cells. It was distributed uniformly within the cytoplasm of uni- and bicellular pollen grains and infected the generative cell. In mature pollen grains, characterized by their triangular shape, the virus was located mainly at the apertures, suggesting that PNRSV distribution follows the same pattern as the cellular components required for pollen tube germination and cell wall tube synthesis. PNRSV also was localized inside pollen tubes, especially in the growth zone. In vitro experiments demonstrated that infection with PNRSV decreases the germination percentage of pollen grains by more than half and delays the growth of pollen tubes by ≈24 h. However, although PNRSV infection affected apricot pollen grain performance during germination, the presence of the virus did not completely prevent fertilization, because the infected apricot pollen tubes, once germinated, were able to reach the apricot embryo sacs, which, in the climatic conditions of southeastern Spain, mature later than in other climates. Thus, infected pollen still could play an important role in the vertical transmission of PNRSV in apricot.

scholarly journals Immunochemical and immunocytochemical identification of a myosin heavy chain polypeptide in Nicotiana pollen tubes

1989 ◽  
Vol 92 (4) ◽  
pp. 569-574
Author(s):  
X.J. Tang ◽  
P.K. Hepler ◽  
S.P. Scordilis
Keyword(s):  
Pollen Tube ◽  
Nuclear Envelope ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Immunofluorescence Microscopy ◽  
Generative Cell ◽  
Pollen Tubes ◽  
Nuclear Surface ◽  
Common Pattern ◽  
Western Blots

A myosin heavy chain polypeptide has been identified and localized in Nicotiana pollen tubes using monoclonal anti-myosin antibodies. The epitopes of these antibodies were found to reside on the myosin heavy chain head and rod portion and were, therefore, designated anti-S-1 (myosin S-1) and anti-LMM (light meromyosin). On Western blots of the total soluble pollen tube proteins, both anti-S-1 and anti-LMM label a polypeptide of approximately 175,000 Mr. Immunofluorescence microscopy shows that both antibodies yield numerous fluorescent spots throughout the whole length of the tube, often with an enrichment in the tube tip. These fluorescent spots are thought to represent vesicles and/or organelles in the pollen tubes. In addition to this common pattern, anti-S-1 stains both the generative cell and the vegetative nuclear envelope. The different staining patterns of the nucleus between anti-S-1 and anti-LMM may be caused by some organization and/or anchorage state of the myosin molecules on the nuclear surface that differs from those on the vesicles and/or organelles.

scholarly journals Tobacco pollen tubes – a fast and easy tool for studying lipid droplet association of plant proteins

2017 ◽  
Vol 89 (5) ◽  
pp. 1055-1064 ◽  
Author(s):  
Anna Ophelia Müller ◽  
Katharina Franziska Blersch ◽  
Anna Lena Gippert ◽  
Till Ischebeck
Keyword(s):  
Lipid Droplet ◽  
Pollen Tubes ◽  
Plant Proteins ◽  
Tobacco Pollen

scholarly journals Identification and Characterization of a Novel Microtubule-Based Motor Associated with Membranous Organelles in Tobacco Pollen Tubes

2000 ◽  
Vol 12 (9) ◽  
pp. 1719-1736 ◽  
Author(s):  
Giampiero Cai ◽  
Silvia Romagnoli ◽  
Alessandra Moscatelli ◽  
Elisa Ovidi ◽  
Gabriella Gambellini ◽  
...  
Keyword(s):  
Pollen Tubes ◽  
Tobacco Pollen ◽  
Identification And Characterization
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