scholarly journals Tobacco pollen tubes – a fast and easy tool for studying lipid droplet association of plant proteins

2017 ◽  
Vol 89 (5) ◽  
pp. 1055-1064 ◽  
Author(s):  
Anna Ophelia Müller ◽  
Katharina Franziska Blersch ◽  
Anna Lena Gippert ◽  
Till Ischebeck
Keyword(s):  
Lipid Droplet ◽  
Pollen Tubes ◽  
Plant Proteins ◽  
Tobacco Pollen

DIACYLGLYCEROL KINASE 5 regulates polar tip growth of tobacco pollen tubes

2021 ◽  
Author(s):  
Patricia Scholz ◽  
Přemysl Pejchar ◽  
Max Fernkorn ◽  
Eliška Škrabálková ◽  
Roman Pleskot ◽  
...  
Keyword(s):  
Tip Growth ◽  
Diacylglycerol Kinase ◽  
Pollen Tubes ◽  
Tobacco Pollen ◽  
Polar Tip Growth

Morphology of Cell Injury: An Approach to the EDIT Programme by the Use of Tobacco Pollen Tubes

2002 ◽  
Vol 30 (3) ◽  
pp. 323-329 ◽  
Author(s):  
Udo Kristen ◽  
Natalie Bischoff ◽  
Saskia Lisboa ◽  
Enno Schirmer ◽  
Sören Witt ◽  
...  
Keyword(s):  
Tip Growth ◽  
Cell Injury ◽  
Cytosolic Calcium ◽  
Pollen Tubes ◽  
In Vitro Cytotoxicity ◽  
Toxic Compounds ◽  
In Vitro System ◽  
Tobacco Pollen ◽  
Different Types

Tobacco pollen tubes were used as a standard in vitro system to investigate cell growth aberrations caused by some of the Multicentre Evaluation of In Vitro Cytotoxicity (MEIC) programme chemicals and other toxic compounds. Changes in cytoskeletal pattern were observed in the tube cells by using tubu-lin immunofluorescence and rhodamin–phalloidin fluorescence for the localisation of microtubules and actin filaments, respectively. Four different types of cell malformation were found: screw-like growth, isodiametric tip swelling, hook formation, and pollen grain enlargement. We suggest that these malformations resulted from an interference by the chemicals with the cytosolic calcium gradient which controls tip growth and the orientation of the pollen tube. The results may contribute to a general understanding of toxicity-based cell malformations.

scholarly journals Identification and Characterization of a Novel Microtubule-Based Motor Associated with Membranous Organelles in Tobacco Pollen Tubes

2000 ◽  
Vol 12 (9) ◽  
pp. 1719-1736 ◽  
Author(s):  
Giampiero Cai ◽  
Silvia Romagnoli ◽  
Alessandra Moscatelli ◽  
Elisa Ovidi ◽  
Gabriella Gambellini ◽  
...  
Keyword(s):  
Pollen Tubes ◽  
Tobacco Pollen ◽  
Identification And Characterization

Heat-shock protein 70 binds microtubules and interacts with kinesin in tobacco pollen tubes

Cytoskeleton ◽  
2013 ◽  
Vol 70 (9) ◽  
pp. 522-537 ◽  
Author(s):  
Luigi Parrotta ◽  
Mauro Cresti ◽  
Giampiero Cai
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Pollen Tubes ◽  
Tobacco Pollen

scholarly journals Analysis of Exocyst Subunit EXO70 Family Reveals Distinct Membrane Polar Domains in Tobacco Pollen Tubes

2017 ◽  
Vol 173 (3) ◽  
pp. 1659-1675 ◽  
Author(s):  
Juraj Sekereš ◽  
Přemysl Pejchar ◽  
Jiří Šantrůček ◽  
Nemanja Vukašinović ◽  
Viktor Žárský ◽  
...  
Keyword(s):  
Pollen Tubes ◽  
Tobacco Pollen ◽  
Distinct Membrane

Cold stress affects cell wall deposition and growth pattern in tobacco pollen tubes

Plant Science ◽  
2019 ◽  
Vol 283 ◽  
pp. 329-342 ◽  
Author(s):  
Luigi Parrotta ◽  
Claudia Faleri ◽  
Gea Guerriero ◽  
Giampiero Cai
Keyword(s):  
Cell Wall ◽  
Cold Stress ◽  
Growth Pattern ◽  
Pollen Tubes ◽  
Wall Deposition ◽  
Tobacco Pollen ◽  
Cell Wall Deposition

scholarly journals Microtubules play a role in trafficking prevacuolar compartments to vacuoles in tobacco pollen tubes

Open Biology ◽  
2018 ◽  
Vol 8 (10) ◽  
pp. 180078 ◽  
Author(s):  
Elisabetta Onelli ◽  
Monica Scali ◽  
Marco Caccianiga ◽  
Nadia Stroppa ◽  
Piero Morandini ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Pollen Tube ◽  
Biochemical Analysis ◽  
Pollen Tubes ◽  
Degradation Pathways ◽  
Transient Transformation ◽  
Secretory Pathways ◽  
Tobacco Pollen ◽  
Fine Regulation ◽  
Pollen Tube Elongation

Fine regulation of exocytosis and endocytosis plays a basic role in pollen tube growth. Excess plasma membrane secreted during pollen tube elongation is known to be retrieved by endocytosis and partially reused in secretory pathways through the Golgi apparatus. Dissection of endocytosis has enabled distinct degradation pathways to be identified in tobacco pollen tubes and has shown that microtubules influence the transport of plasma membrane internalized in the tip region to vacuoles. Here, we used different drugs affecting the polymerization state of microtubules together with SYP21, a marker of prevacuolar compartments, to characterize trafficking of prevacuolar compartments in Nicotiana tabacum pollen tubes. Ultrastructural and biochemical analysis showed that microtubules bind SYP21-positive microsomes. Transient transformation of pollen tubes with LAT52-YFP-SYP21 revealed that microtubules play a key role in the delivery of prevacuolar compartments to tubular vacuoles.

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