An electron microscope autoradiographic study of DOC conversion to corticosterone in the rat adrenal cortex

1974 ◽  
Vol 151 (1) ◽  
Author(s):  
MariaC. Magalh�es ◽  
M.M. Magalh�es ◽  
A. Coimbra
Keyword(s):  
Electron Microscope ◽  
Adrenal Cortex ◽  
Autoradiographic Study

scholarly journals Electron microscope autoradiographic study of intestinal absorption of decanoic and octanoic acids in the rat.

1975 ◽  
Vol 65 (2) ◽  
pp. 383-397 ◽  
Author(s):  
H Carlier ◽  
J Bezard
Keyword(s):  
Fatty Acids ◽  
Electron Microscope ◽  
Intestinal Absorption ◽  
Common Duct ◽  
Autoradiographic Study ◽  
Electron Microscope Autoradiography ◽  
Blood Capillaries ◽  
Medium Chain ◽  
Medium Chain Fatty Acids ◽  
Chain Fatty Acids

Intestinal absorption of [3H]octanoic acid and [3H]decanoic acid was investigated in the rat by electron microscope autoradiography. The common duct (bile and pancreatic common duct) of the rats was diverted and a loop of the duodenum was cannulated 24 h later. The lipid mixture to be investigated was introduced into each experimental loop, and after 15 min or less the loop was removed. One part of each loop was used to determine the distribution of radioactivity in different lipid fractions, and an autoradiographic study was performed on the other part of the loop. Radioactivity distribution studies confirmed that medium chain fatty acids are absorbed in their nonesterified form and established that these fatty acids are absorbed much more rapidly than oleic acid. Autoradiographic studies indicated that the medium chain fatty acids are taken up in a molecular or aggregate molecular form, leave the epithelial cells by way of the lateral plasma membrane, and are next found in the blood capillaries. Our results suggest that the Golgi complex does not play an important role in the absorption of unesterified fatty acids.

CELL PROLIFERATION IN THE PREPUBERTAL MALE RAT ADRENAL CORTEX: AN AUTORADIOGRAPHIC STUDY

1971 ◽  
Vol 49 (4) ◽  
pp. 599-609 ◽  
Author(s):  
N. A. WRIGHT
Keyword(s):  
Cell Cycle ◽  
Cell Proliferation ◽  
Adrenal Cortex ◽  
Tritiated Thymidine ◽  
Autoradiographic Study ◽  
Male Rats ◽  
Male Rat ◽  
General Increase ◽  
Cortical Cells ◽  
Cell Cycle Time

SUMMARY On the basis of labelling indices measured with tritiated thymidine at intervals throughout its thickness, the adrenal cortex of prepubertal male rats has been divided into four compartments. These are called the glomerular, proliferative, fascicular and reticular compartments, respectively. Labelling indices measured for each compartment showed highest values in the glomerular and proliferative compartments, with values of 6·73% and 7·09% respectively. The fascicular compartment showed a lower index of 3·16% while the reticular compartment gave the lowest value of 1·15%. These differences are further reflected in measurements of the mitotic indices for each compartment. The phases of the cell cycle have been measured by pulse-chase analysis in each compartment, and all phases estimated showed an increase in duration as the inner compartments were approached. The duration of interphase DNA synthesis (ts) was found to be shortest in the glomerular and proliferative compartments, with values of 7·45 and 7·73 h, respectively. The fascicular compartment showed lengthening of ts to 8·56 h, and the reticular compartment gave the highest value of 9·21 h. Similarly, the values obtained for G2 (the post-DNA synthetic interval) and tm (the duration of mitosis), and a calculated value of the cell cycle time all showed a general increase in duration from the outer to the inner compartments. The relation of these results to theories of adrenocortical cytogenesis is discussed, and it is suggested that the differences in cell cycle components can best be explained by the inward migration of cortical cells from the outer compartments.

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