scholarly journals On nucleolar DNA replication in Tetrahymena pyriformis GL-8: An electron microscope autoradiographic study of heat synchronized and exponentially multiplying cells

1980 ◽  
Vol 45 (2) ◽  
pp. 101-111 ◽  
Author(s):  
Jytte R. Nilsson ◽  
Erik Zeuthen
Keyword(s):  
Electron Microscope ◽  
Dna Replication ◽  
Tetrahymena Pyriformis ◽  
Autoradiographic Study

scholarly journals Electron microscope autoradiographic study of intestinal absorption of decanoic and octanoic acids in the rat.

1975 ◽  
Vol 65 (2) ◽  
pp. 383-397 ◽  
Author(s):  
H Carlier ◽  
J Bezard
Keyword(s):  
Fatty Acids ◽  
Electron Microscope ◽  
Intestinal Absorption ◽  
Common Duct ◽  
Autoradiographic Study ◽  
Electron Microscope Autoradiography ◽  
Blood Capillaries ◽  
Medium Chain ◽  
Medium Chain Fatty Acids ◽  
Chain Fatty Acids

Intestinal absorption of [3H]octanoic acid and [3H]decanoic acid was investigated in the rat by electron microscope autoradiography. The common duct (bile and pancreatic common duct) of the rats was diverted and a loop of the duodenum was cannulated 24 h later. The lipid mixture to be investigated was introduced into each experimental loop, and after 15 min or less the loop was removed. One part of each loop was used to determine the distribution of radioactivity in different lipid fractions, and an autoradiographic study was performed on the other part of the loop. Radioactivity distribution studies confirmed that medium chain fatty acids are absorbed in their nonesterified form and established that these fatty acids are absorbed much more rapidly than oleic acid. Autoradiographic studies indicated that the medium chain fatty acids are taken up in a molecular or aggregate molecular form, leave the epithelial cells by way of the lateral plasma membrane, and are next found in the blood capillaries. Our results suggest that the Golgi complex does not play an important role in the absorption of unesterified fatty acids.

Timing of nucleolar DNA replication in Amoeba proteus

1976 ◽  
Vol 22 (3) ◽  
pp. 521-530
Author(s):  
I. Minassian ◽  
L.G. Bell
Keyword(s):  
Electron Microscope ◽  
Dna Replication ◽  
Dna Synthesis ◽  
Central Region ◽  
Thymidine Incorporation ◽  
Tritiated Thymidine ◽  
Amoeba Proteus ◽  
Electron Microscope Autoradiography ◽  
Microscope Autoradiography ◽  
G2 Phase

Light- and electron-microscope autoradiography have been used to follow the incorporation of [3H]thymidine at different stages during the interphase of synchronously growing populations of Amoeba proteus. Two main patterns were found for tritiated thymidine incorporation, i.e. DNA synthesis. The major incorporation was in the central region of the nucleus, but a lesser degree of incorporation occurred in the nucleolar region. The bulk of this nucleolar DNA was found to be late replicating, i.e. it replicated during the G2 phase.

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