Ultrastructure of cell surface coat (glycocalyx) in rat urinary bladder epithelium

1975 ◽  
Vol 158 (2) ◽  
Author(s):  
Stuart Levin ◽  
WardR. Richter
Keyword(s):  
Urinary Bladder ◽  
Cell Surface ◽  
Surface Coat ◽  
Bladder Epithelium ◽  
Rat Urinary Bladder

Change of membrane potential in rat urinary bladder epithelium treated with sodium l-ascorbate

1992 ◽  
Vol 63 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Makoto Asamoto ◽  
Katsumi Imaida ◽  
Ryohei Hasegawa ◽  
Ken Hotta ◽  
Shoji Fukushima
Keyword(s):  
Membrane Potential ◽  
Urinary Bladder ◽  
Bladder Epithelium ◽  
Rat Urinary Bladder

Expression and localization of epithelial sodium channel in mammalian urinary bladder

1998 ◽  
Vol 274 (1) ◽  
pp. F91-F96 ◽  
Author(s):  
Peter R. Smith ◽  
Scott A. Mackler ◽  
Philip C. Weiser ◽  
David R. Brooker ◽  
Yoon J. Ahn ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Urinary Bladder ◽  
Mrna Expression ◽  
Northern Blot ◽  
Apical Membrane ◽  
Bladder Epithelium ◽  
Rat Urinary Bladder ◽  
Luminal Membrane ◽  
Electrophysiological Studies

The mammalian urinary bladder exhibits transepithelial Na+ absorption that contributes to Na+ gradients established by the kidney. Electrophysiological studies have demonstrated that electrogenic Na+ absorption across the urinary bladder is mediated in part by amiloride-sensitive Na+ channels situated within the apical membrane of the bladder epithelium. We have used a combination of in situ hybridization, Northern blot analysis, and immunocytochemistry to examine whether the recently cloned epithelial Na+ channel (ENaC) is expressed in the rat urinary bladder. In situ hybridization and Northern blot analyses indicate that α-, β-, and γ-rat ENaC (rENaC) are expressed in rat urinary bladder epithelial cells. Quantitation of the levels of α-, β-, and γ-rENaC mRNA expression in rat urinary bladder, relative to β-actin mRNA expression, indicates that, although comparable levels of α- and β-rENaC subunits are expressed in the urinary bladder of rats maintained on standard chow, the level of γ-rENaC mRNA expression is 5- to 10-fold lower than α- or β-rENaC mRNA. Immunocytochemistry, using an antibody directed against α-rENaC, revealed that ENaCs are predominantly localized to the luminal membrane of the bladder epithelium. Together, these data demonstrate that ENaC is expressed in the mammalian urinary bladder and suggest that amiloride-sensitive Na+ transport across the apical membrane of the mammalian urinary bladder epithelium is mediated primarily by ENaC.

scholarly journals In situFreezing of the Rat Urinary Bladder: DNA Adduct Formation in the Bladder Epithelium Demonstrated by32P-Postlabeling Assay

1989 ◽  
Vol 80 (9) ◽  
pp. 826-832 ◽  
Author(s):  
Ryohei Hasegawa ◽  
Katsumi Yamashita ◽  
Kazushige Morimoto ◽  
Fumio Furukawa ◽  
Kazuhiro Toyoda ◽  
...  
Keyword(s):  
Urinary Bladder ◽  
Adduct Formation ◽  
Dna Adduct ◽  
Bladder Epithelium ◽  
Rat Urinary Bladder

scholarly journals Immunofluorescence microscopic study on the cytoskeletal filaments in the separated superficial cells of rat urinary bladder epithelium.

1991 ◽  
Vol 24 (1) ◽  
pp. 21-27 ◽  
Author(s):  
SADAHIRO WATANABE ◽  
JUNZO SASAKI ◽  
TOSHIYUKI KOUNO ◽  
TAKAO OKADA ◽  
NAGAYASU OTSUKA
Keyword(s):  
Urinary Bladder ◽  
Microscopic Study ◽  
Bladder Epithelium ◽  
Rat Urinary Bladder
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