12-O-tetradecanoylphorbol-13-acetate induces selective desquamation of superficial cells in rat urinary bladder epithelium

1992 ◽  
Vol 268 (2) ◽  
pp. 239-245 ◽  
Author(s):  
S. Watanabe ◽  
J. Sasaki
1992 ◽  
Vol 63 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Makoto Asamoto ◽  
Katsumi Imaida ◽  
Ryohei Hasegawa ◽  
Ken Hotta ◽  
Shoji Fukushima

1998 ◽  
Vol 274 (1) ◽  
pp. F91-F96 ◽  
Author(s):  
Peter R. Smith ◽  
Scott A. Mackler ◽  
Philip C. Weiser ◽  
David R. Brooker ◽  
Yoon J. Ahn ◽  
...  

The mammalian urinary bladder exhibits transepithelial Na+ absorption that contributes to Na+ gradients established by the kidney. Electrophysiological studies have demonstrated that electrogenic Na+ absorption across the urinary bladder is mediated in part by amiloride-sensitive Na+ channels situated within the apical membrane of the bladder epithelium. We have used a combination of in situ hybridization, Northern blot analysis, and immunocytochemistry to examine whether the recently cloned epithelial Na+ channel (ENaC) is expressed in the rat urinary bladder. In situ hybridization and Northern blot analyses indicate that α-, β-, and γ-rat ENaC (rENaC) are expressed in rat urinary bladder epithelial cells. Quantitation of the levels of α-, β-, and γ-rENaC mRNA expression in rat urinary bladder, relative to β-actin mRNA expression, indicates that, although comparable levels of α- and β-rENaC subunits are expressed in the urinary bladder of rats maintained on standard chow, the level of γ-rENaC mRNA expression is 5- to 10-fold lower than α- or β-rENaC mRNA. Immunocytochemistry, using an antibody directed against α-rENaC, revealed that ENaCs are predominantly localized to the luminal membrane of the bladder epithelium. Together, these data demonstrate that ENaC is expressed in the mammalian urinary bladder and suggest that amiloride-sensitive Na+ transport across the apical membrane of the mammalian urinary bladder epithelium is mediated primarily by ENaC.


1989 ◽  
Vol 80 (9) ◽  
pp. 826-832 ◽  
Author(s):  
Ryohei Hasegawa ◽  
Katsumi Yamashita ◽  
Kazushige Morimoto ◽  
Fumio Furukawa ◽  
Kazuhiro Toyoda ◽  
...  

1991 ◽  
Vol 24 (1) ◽  
pp. 21-27 ◽  
Author(s):  
SADAHIRO WATANABE ◽  
JUNZO SASAKI ◽  
TOSHIYUKI KOUNO ◽  
TAKAO OKADA ◽  
NAGAYASU OTSUKA

Urology ◽  
2003 ◽  
Vol 61 (1) ◽  
pp. 236-242 ◽  
Author(s):  
Şule Çetinel ◽  
B.lent Çetinel ◽  
Feriha Ercan ◽  
Canan Hrda ◽  
Tangl Şan

Sign in / Sign up

Export Citation Format

Share Document