Effect of ATP inhibitors on the translocation of luminal membrane between cytoplasm and cell surface of transitional epithelial cells during the expansion-contraction cycle of the rat urinary bladder

1986 ◽  
Vol 246 (1) ◽  
Author(s):  
StephenN. Sarikas ◽  
FrancisJ. Chlapowski
Keyword(s):  
Epithelial Cells ◽  
Urinary Bladder ◽  
Cell Surface ◽  
Rat Urinary Bladder ◽  
Luminal Membrane ◽  
Contraction Cycle

Structure of the Subunits in the Thick Luminal Membrane of Rat Urinary Bladder

Nature ◽  
1970 ◽  
Vol 227 (5255) ◽  
pp. 280-281 ◽  
Author(s):  
R. C. WARREN ◽  
R. M. HICKS
Keyword(s):  
Urinary Bladder ◽  
Rat Urinary Bladder ◽  
Luminal Membrane

Expression and localization of epithelial sodium channel in mammalian urinary bladder

1998 ◽  
Vol 274 (1) ◽  
pp. F91-F96 ◽  
Author(s):  
Peter R. Smith ◽  
Scott A. Mackler ◽  
Philip C. Weiser ◽  
David R. Brooker ◽  
Yoon J. Ahn ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Urinary Bladder ◽  
Mrna Expression ◽  
Northern Blot ◽  
Apical Membrane ◽  
Bladder Epithelium ◽  
Rat Urinary Bladder ◽  
Luminal Membrane ◽  
Electrophysiological Studies

The mammalian urinary bladder exhibits transepithelial Na+ absorption that contributes to Na+ gradients established by the kidney. Electrophysiological studies have demonstrated that electrogenic Na+ absorption across the urinary bladder is mediated in part by amiloride-sensitive Na+ channels situated within the apical membrane of the bladder epithelium. We have used a combination of in situ hybridization, Northern blot analysis, and immunocytochemistry to examine whether the recently cloned epithelial Na+ channel (ENaC) is expressed in the rat urinary bladder. In situ hybridization and Northern blot analyses indicate that α-, β-, and γ-rat ENaC (rENaC) are expressed in rat urinary bladder epithelial cells. Quantitation of the levels of α-, β-, and γ-rENaC mRNA expression in rat urinary bladder, relative to β-actin mRNA expression, indicates that, although comparable levels of α- and β-rENaC subunits are expressed in the urinary bladder of rats maintained on standard chow, the level of γ-rENaC mRNA expression is 5- to 10-fold lower than α- or β-rENaC mRNA. Immunocytochemistry, using an antibody directed against α-rENaC, revealed that ENaCs are predominantly localized to the luminal membrane of the bladder epithelium. Together, these data demonstrate that ENaC is expressed in the mammalian urinary bladder and suggest that amiloride-sensitive Na+ transport across the apical membrane of the mammalian urinary bladder epithelium is mediated primarily by ENaC.

In vitro neoplastic transformation of epithelial cells of rat urinary bladder by nitrosamines

Nature ◽  
1974 ◽  
Vol 252 (5483) ◽  
pp. 497-499 ◽  
Author(s):  
YOSHIYUKI HASHIMOTO ◽  
HISAYO S. KITAGAWA
Keyword(s):  
Epithelial Cells ◽  
Urinary Bladder ◽  
Neoplastic Transformation ◽  
Rat Urinary Bladder
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