Permeabilization of the plasmalemma and wall of soybean root cells to macromolecules

Planta ◽  
1991 ◽  
Vol 184 (4) ◽  
Author(s):  
Sally Meiners ◽  
ParamjitK. Gharyal ◽  
Melvin Schindler
Keyword(s):  
Root Cells ◽  
Soybean Root

Investigations of growth-promoting factors in conditioned soybean root cells and in the liquid medium in which they grow: ammonium, glutamine, and amino acids

1974 ◽  
Vol 52 (7) ◽  
pp. 1747-1755 ◽  
Author(s):  
P. A. Sargent ◽  
J. King
Keyword(s):  
Amino Acids ◽  
Dry Weight ◽  
Liquid Media ◽  
Root Cells ◽  
Nitrogenous Compounds ◽  
Soybean Root ◽  
Haplopappus Gracilis ◽  
Glycine Max L ◽  
Growth Promoting ◽  
Cells Cultured

Cells cultured in sterile, liquid media from a number of Phaseolus spp., soybean cotyledons, shoots, and roots and from rice explants grew, in terms of dry-weight increase, much better in the presence of NH4+ and NO3− as sources of nitrogen than with NO3− alone. Other cultures tested, including other legumes, either did not respond positively to added NH4+ or, as in the case of Haplopappus gracilis cells, grew better in its absence.Earlier it had been shown that soybean (Glycine max. L. cv. Mandarin) root cells grew better in the presence of NH4+ than in its absence and that 'conditioning' substances were produced by cells and excreted into the medium between about the 15th and 35th h of incubation. These observations and those above with other cell cultures led to the initiation of an investigation of why some cells respond to NH4+ while others do not.This investigation has so far taken the form of an analysis of nitrogenous compounds in soybean root cells and in the NH4+-containing medium in which they were grown during 120 h of incubation and especially after 24 h of incubation, the time of maximum production of growth-enhancing ability in both cells and medium.Growth enhancement can be accounted for, apparently, by the occurrence of residual NH4+ in conditioned medium and by the presumed occurrence of NH4+ in cells. However, glutamine and its derivatives are implicated in the conditioning process.

scholarly journals Auxin Transport in Suspension-Cultured Soybean Root Cells

1991 ◽  
Vol 96 (1) ◽  
pp. 192-197 ◽  
Author(s):  
Matthew T. Loper ◽  
Roger M. Spanswick
Keyword(s):  
Auxin Transport ◽  
Root Cells ◽  
Soybean Root

Endoplasmic Reticulum Forms a Dynamic Continuum for Lipid Diffusion between Contiguous Soybean Root Cells

1993 ◽  
Vol 5 (1) ◽  
pp. 25 ◽  
Author(s):  
Sharon Grabski ◽  
Adriaan W. de Feijter ◽  
Melvin Schindler
Keyword(s):  
Endoplasmic Reticulum ◽  
Root Cells ◽  
Soybean Root ◽  
Lipid Diffusion

Invasion of Soybean Root Cells in Culture by Rhizobium japonicum.

1975 ◽  
Vol 33 ◽  
pp. 582-583
Author(s):  
Dan Raveed ◽  
Minocher Reporter ◽  
Grace Norris
Keyword(s):  
Cell Walls ◽  
Plant Cells ◽  
Culture Conditions ◽  
Final Concentration ◽  
Rhizobium Japonicum ◽  
Symbiotic Association ◽  
Extracellular Material ◽  
Root Cells ◽  
Soybean Root ◽  
Soybean Cell

The initiation of association between cultured soybean root cells and Rhizobia shows specificity under proper conditions of culture. The establishment of these culture conditions for symbiotic association have been described previously. The compatible plant cells produced a filamentous extracellular material which was capable of trapping Rhizobia. Non-compatible cells did not produce extracellular material and were not invaded. In this presentation we have followed the effect of Rhizobial invasion on the wall morphology of the soybean root cells in suspension cultures. Changes in the morphology of the soybean cell walls were then examined as follows.Harosoy root cells were grown in liquid culture in Gamborg's B-5 medium for two weeks. Rhizobium japonicum strain 138 was added to the culture for another 3 days. For this purpose, fixation was carried out by adding glutaraldehyde to the medium to a final concentration of 2%. The cell clumps were fixed 1 hr.

Amino acid transport systems of cultured soybean root cells

1975 ◽  
Vol 53 (18) ◽  
pp. 2088-2091 ◽  
Author(s):  
J. King ◽  
Rozina Hirji
Keyword(s):  
Amino Acids ◽  
Glycine Max ◽  
Amino Acid ◽  
Amino Acid Transport ◽  
Transport Systems ◽  
Acid Transport ◽  
Root Cells ◽  
Soybean Root ◽  
Neutral Amino Acids ◽  
Glycine Max L

The uptake of 1 μM14C-labelled arginine, glutamate, and alanine by cultured soybean (Glycine max L. cv. Mandarin) root cells was followed for periods up to 4 min at pH 5.5 in the presence of a 10 μM concentration of other amino compounds. From the degree of competition between 14C-labelled and unlabelled amino acids a number of uptake systems for basic, acidic, and neutral amino acids were identified, and a number of problems associated with amino acid transport in soybean cells were uncovered.

Binding of Rhizobium japonicum to cultured soybean root cells: Morphological evidence

1975 ◽  
Vol 5 (1) ◽  
pp. 73-76 ◽  
Author(s):  
Minocher Reporter ◽  
Dan Raveed ◽  
Grace Norris
Keyword(s):  
Rhizobium Japonicum ◽  
Morphological Evidence ◽  
Root Cells ◽  
Soybean Root

scholarly journals Auxin Transport in Suspension-Cultured Soybean Root Cells

1991 ◽  
Vol 96 (1) ◽  
pp. 184-191 ◽  
Author(s):  
Matthew T. Loper ◽  
Roger M. Spanswick
Keyword(s):  
Auxin Transport ◽  
Root Cells ◽  
Soybean Root

scholarly journals Compatible and Incompatible Rhizobia Alter Membrane Potentials of Soybean Root Cells

1986 ◽  
Vol 82 (4) ◽  
pp. 1115-1118 ◽  
Author(s):  
Tibor Érsek ◽  
Anton Novacky ◽  
Steven G. Pueppke
Keyword(s):  
Membrane Potentials ◽  
Root Cells ◽  
Soybean Root

Nutrient requirements of suspension cultures of soybean root cells

1968 ◽  
Vol 50 (1) ◽  
pp. 151-158 ◽  
Author(s):  
O.L. Gamborg ◽  
R.A. Miller ◽  
K. Ojima
Keyword(s):  
Suspension Cultures ◽  
Nutrient Requirements ◽  
Root Cells ◽  
Soybean Root
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