Mitochondrial citrate synthase from potato: predominant expression in mature leaves and young flower buds

Planta ◽  
1995 ◽  
Vol 196 (4) ◽  
Author(s):  
Volker Landsch�tze ◽  
Bernd M�ller-R�ber ◽  
Lothar Willmitzer
Planta ◽  
1995 ◽  
Vol 196 (4) ◽  
pp. 756-764 ◽  
Author(s):  
Volker Landsch�tze ◽  
Bernd M�ller-R�ber ◽  
Lothar Willmitzer

1972 ◽  
Vol 52 (4) ◽  
pp. 643-649 ◽  
Author(s):  
R. A. HAMLEN ◽  
F. L. LUKEZIC ◽  
J. R. BLOOM

Influence of clipping height on neutral carbohydrate levels in root exudates of alfalfa grown gnotobiotically was investigated by gas-chromatographic and mass-spectral techniques. Exudates were obtained from plants that were lightly clipped (removal of flower buds), intermediately clipped (15 cm), and severely clipped (removal of all but four mature leaves). Glucose, inositol, sucrose, and four unidentified (U) components were detected. Fluctuations in the level of sugars were observed in exudates from two sampling periods. Glucose and U1 were most concentrated under light clipping. Levels of inositol and sucrose were maximum under intermediate clipping. Amounts of U3 were greatest from severely clipped plants, whereas levels of U5 remained constant at all cuttings. Concentration of U4 was lowest under severe clipping. U2, present in exudates from seedling plants, was not detected. Quantities of sugars released per gram of dry weight of root tissue were greater under severe clipping. U1 was the major component, with glucose, inositol, and sucrose minor components of the total sugars.


Crop Science ◽  
1978 ◽  
Vol 18 (6) ◽  
pp. 1071-1074 ◽  
Author(s):  
B. W. Hanny ◽  
W. R. Meredith ◽  
Jack C. Bailey ◽  
A. J. Harvey

IAWA Journal ◽  
2014 ◽  
Vol 35 (3) ◽  
pp. 281-292
Author(s):  
Kishore S. Rajput ◽  
Amreen Saiyed ◽  
Vidya S. Patil ◽  
K.S. Rao

Peduncles of Couroupita guianensis Aubl. undergo extensive secondary growth, which is a rare and unexplored feature so far. In the present investigation seasonal behaviour of vascular cambium was studied in fruit-bearing peduncles and compared with the vegetative branches of similar diameter. In peduncles, the cambium remained active throughout the year. The number of cambium cells and differentiating xylem cells increased from May and reached a maximum in July-August. Although cambial growth occurred throughout the year, it was relatively sluggish in February despite the development of new leaves and ongoing extension growth. In contrast, cambial cell division in young branches initiated in February, peaked in the same months as peduncle cambium while cambial cell division and differentiation of xylem remained suspended from October to January. Cessation of cambial cell division in the branches during this period may be correlated with the presence of mature leaves. In both (branches and peduncle), rapid cell division and increase in the number of differentiating xylem elements in April-May is positively correlated with the development of flower buds and new leaves. The present anatomical investigation revealed that cambial activity in both peduncle and vegetative branches are independent of phenology and climatic conditions. In conclusion, we believe that variations in the number of differentiating cambium derivatives in peduncles benefits from a dual source of growth hormone supply, i.e. from developing new leaves and flower buds.


PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4812 ◽  
Author(s):  
Li Cai ◽  
Lu Zhang ◽  
Qiantang Fu ◽  
Zeng-Fu Xu

The seed oil of Jatropha curcas is considered a potential bioenergy source that could replace fossil fuels. However, the seed yield of Jatropha is low and has yet to be improved. We previously reported that exogenous cytokinin treatment increased the seed yield of Jatropha. Cytokinin levels are directly regulated by isopentenyl transferase (IPT), cytochrome P450 monooxygenase, family 735, subfamily A (CYP735A), and cytokinin oxidase/dehydrogenase (CKX). In this study, we cloned six IPT genes, one JcCYP735A gene, and seven JcCKX genes. The expression patterns of these 14 genes in various organs were determined using real-time quantitative PCR. JcIPT1 was primarily expressed in roots and seeds, JcIPT2 was expressed in roots, apical meristems, and mature leaves, JcIPT3 was expressed in stems and mature leaves, JcIPT5 was expressed in roots and mature leaves, JcIPT6 was expressed in seeds at 10 days after pollination, and JcIPT9 was expressed in mature leaves. JcCYP735A was mainly expressed in roots, flower buds, and seeds. The seven JcCKX genes also showed different expression patterns in different organs of Jatropha. In addition, CK levels were detected in flower buds and seeds at different stages of development. The concentration of N6-(Δ2-isopentenyl)-adenine (iP), iP-riboside, and trans-zeatin (tZ) increased with flower development, and the concentration of iP decreased with seed development, while that of tZ increased. We further analyzed the function of JcCYP735A using the CRISPR-Cas9 system, and found that the concentrations of tZ and tZ-riboside decreased significantly in the Jccyp735a mutants, which showed severely retarded growth. These findings will be helpful for further studies of the functions of cytokinin metabolic genes and understanding the roles of cytokinins in Jatropha growth and development.


HortScience ◽  
1994 ◽  
Vol 29 (7) ◽  
pp. 738f-738
Author(s):  
Ricardo Campos ◽  
Ma. Estela Peralta ◽  
Daniel W. Bearden ◽  
William B. Miller

Soluble carbohydrate extracts from Antirrhinum majus L. leaves were fractionated by ion exchange chromatography. Putative mannitol was tentatively identified by retention behavior on two high performance liquid chromatography columns. Mannitol was confirmed using 1H and 13C nuclear magnetic resonance (NMR), and by gas chromatography (GC) and mass spectroscopy (MS). The melting point of authentic and putative mannitol, and a 1:1 mix was from 164 to 166°C. Using the EDTA-phloem exudate technique, mannitol was detected in phloem tissue associated with mature flowers, flower buds, and mature leaves, suggesting that mannitol is translocated in Antirrhinum.


1956 ◽  
Vol 34 (4) ◽  
pp. 711-719 ◽  
Author(s):  
B. P. Goplen ◽  
J. E. R. Greenshields ◽  
W. J. White

The loss of coumarin from sweet clover leaf samples amounted to 70 to 75% when the samples were air-dried for 10 days and 21 to 51% when oven-dried at 175° F. for one hour. Fresh leaf material placed in 2.5 N NaOH and stored in darkness at room temperature showed no loss of coumarin over a six month period but when stored in light at room temperature losses were evident: in 10 days and very heavy after six months' storage. Coumarin content was maximum in flower buds and fresh leaves from the tip of branches. Root and stem tissue and the more mature leaves from the central and lower portion of the plants were very low in coumarin. A rapid qualitative procedure is described which involves collection of fresh young leaves and storage of them in 2.5 N NaOH in the dark until they are examined in ultraviolet light. Plants classified as coumarin-deficient by this procedure were found to be void of coumarin on photofluorometric analysis.


1997 ◽  
Vol 101 (3) ◽  
pp. 583-590 ◽  
Author(s):  
Na Eun Cheong ◽  
Yeon Ok Choi ◽  
Woe Yeon Kim ◽  
Sun Chang Kim ◽  
Moo Je Cho ◽  
...  

2020 ◽  
Vol 45 (3) ◽  
pp. 537-543
Author(s):  
Karinne Sampaio Valdemarin ◽  
Jair Eustáquio Quintino Faria ◽  
Fiorella Fernanda Mazine ◽  
Vinicius Castro Souza

Abstract—A new species of Eugenia from the Atlantic forest of Brazil is described and illustrated. Eugenia flavicarpa is restricted to the Floresta de Tabuleiro (lowland forests) of Espírito Santo state and is nested in Eugenia subg. Pseudeugenia. Considering all other species of the subgenus that occur in forest vegetation types of the Atlantic forest phytogeographic domain, Eugenia flavicarpa can be distinguished mainly by the combination of smooth leaves with indumentum on both surfaces, with two marginal veins, usually ramiflorous inflorescences, pedicels 4.5‐9.7 mm long, flower buds 3.5‐4 mm in diameter, and by the calyx lobes that are 2‐3 mm long with rounded to obtuse apices. Morphological analyses were performed to explore the significance of quantitative diagnostic features between the new species and the closely related species, Eugenia farneyi. Notes on the habitat, distribution, phenology, and conservation status of Eugenia flavicarpa are provided, as well as a key for all species of Eugenia subg. Pseudeugenia from forest vegetation of the Atlantic forest phytogeographic domain.


Agrotek ◽  
2018 ◽  
Vol 2 (3) ◽  
Author(s):  
Antonius Suparno ◽  
Opalina Logo ◽  
Dwiana Wasgito Purnomo

Sweet potato serves as a staple food for people in Jayawijaya. Many cultivars of sweet potatoes have been cultivated by Dani tribe in Kurulu as foot for their infant, child and adult as well as feeding especially for pigs. Base on the used of sweet potatoes as food source for infant and child, this study explored 10 different cultivars. As for the leaf morphology, it was indentified that the mature leaves have size around 15 � 18 cm. general outline of the leaf is reniform (40%), 60% have green colour leaf, 50% without leaf lobe, 60% of leaf lobes number is one, 70% of shape of central leaf lobe is toothed. Abazial leaf vein pigmentation have purple (40%), and petiole pigmentation is purple with green near leaf (60%), besides its tuber roots, sweet potatoes are also harvested for its shoots and green young leaves for vegetables.


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