Demonstration of Borna disease virus-specific RNA in secretions of naturally infected horses by the polymerase chain reaction

1993 ◽  
Vol 182 (6) ◽  
Author(s):  
J.A. Richt ◽  
S. Herzog ◽  
K. Haberzettl ◽  
R. Rott
Keyword(s):  
Polymerase Chain Reaction ◽  
Disease Virus ◽  
Chain Reaction ◽  
Borna Disease Virus ◽  
Polymerase Chain ◽  
Borna Disease

No borna disease virus-specific RNA detected in blood of race horses and jockeys

2006 ◽  
Vol 18 (3-4) ◽  
pp. 177-180 ◽  
Author(s):  
Yong-Ku Kim ◽  
Kyung-Bo Noh ◽  
Chang-Su Han ◽  
Ju-Young Moon ◽  
Do-Kyung Yoon ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Disease Virus ◽  
Mononuclear Cells ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Peripheral Blood Mononuclear ◽  
Borna Disease Virus ◽  
Polymerase Chain ◽  
Race Horses ◽  
Borna Disease

Background:Borna disease virus (BDV) predominantly infects horses and sheep, causing a broad range of behavioural disorders. It is controversial whether BDV infects humans and causes psychiatric disorders.Objectives:We searched for BDV-derived nucleic acids in blood of race horses and jockeys riding the horses.Methods:We assayed for the BDV genome in RNA extracted from peripheral blood mononuclear cells (PBMC) of 39 race horses and 48 jockeys. Two polymerase chain reaction protocols [one-tube reverse transcription–polymerase chain reaction (RT-PCR) and two-step RT-PCR] were used to assay BDV p24 and p40 transcripts.Results:The p24 and p40 viral nucleic acid sequences were not detected in the PBMC RNAs from any of the race horses or jockeys.Conclusions:These data do not support an epidemiological association between BDV infection, race horses and humans.

scholarly journals Psittacine beak and feather disease virus in budgerigars and ring-neck parakeets in South Africa

2004 ◽  
Vol 71 (1) ◽  
Author(s):  
J. Albertyn ◽  
K.M. Tajbhai ◽  
R.R. Bragg
Keyword(s):  
South Africa ◽  
Polymerase Chain Reaction ◽  
Genetic Variation ◽  
Disease Virus ◽  
Common Disease ◽  
Sample Collection ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Feather Disease Virus ◽  
Viral Isolates

Psittacine beak and feather disease (PBFD) is a common disease of the psittacine species and is caused by the psittacine beak and feather disease virus (PBFDV). In this study the occurrence of the disease in ring-neck parakeets and budgerigars in South Africa suffering from feathering problems, using polymerase chain reaction as a diagnostic test was investigated. The genetic variation between viral isolates was also studied. Results indicate that PBFDV can be attributed to being the cause of feathering problems in some of the ring-neck parakeets and budgerigars in South Africa. Genetic variation of isolates occurs between species and individuals. A cheap and easy to use method of blood sample collection on filter paper for diagnostic purposes was also evaluated. It proved to be less stressful to the birds and did not inhibit further processes.

Sign in / Sign up

Export Citation Format

Share Document