scholarly journals Reverse Transcription Nested Polymerase Chain Reaction for Detecting p40 RNA of Borna Disease Virus, without Risk of Plasmid Contamination.

1999 ◽  
Vol 61 (1) ◽  
pp. 77-80 ◽  
Author(s):  
Tetsuya MIZUTANI ◽  
Yoshii NISHINO ◽  
Hiroaki KARIWA ◽  
Ikuo TAKASHIMA
Keyword(s):  
Polymerase Chain Reaction ◽  
Disease Virus ◽  
Reverse Transcription ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Borna Disease Virus ◽  
Polymerase Chain ◽  
Plasmid Contamination ◽  
Borna Disease

No borna disease virus-specific RNA detected in blood of race horses and jockeys

2006 ◽  
Vol 18 (3-4) ◽  
pp. 177-180 ◽  
Author(s):  
Yong-Ku Kim ◽  
Kyung-Bo Noh ◽  
Chang-Su Han ◽  
Ju-Young Moon ◽  
Do-Kyung Yoon ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Disease Virus ◽  
Mononuclear Cells ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Peripheral Blood Mononuclear ◽  
Borna Disease Virus ◽  
Polymerase Chain ◽  
Race Horses ◽  
Borna Disease

Background:Borna disease virus (BDV) predominantly infects horses and sheep, causing a broad range of behavioural disorders. It is controversial whether BDV infects humans and causes psychiatric disorders.Objectives:We searched for BDV-derived nucleic acids in blood of race horses and jockeys riding the horses.Methods:We assayed for the BDV genome in RNA extracted from peripheral blood mononuclear cells (PBMC) of 39 race horses and 48 jockeys. Two polymerase chain reaction protocols [one-tube reverse transcription–polymerase chain reaction (RT-PCR) and two-step RT-PCR] were used to assay BDV p24 and p40 transcripts.Results:The p24 and p40 viral nucleic acid sequences were not detected in the PBMC RNAs from any of the race horses or jockeys.Conclusions:These data do not support an epidemiological association between BDV infection, race horses and humans.

scholarly journals Pet rat harbouring Seoul hantavirus in Sweden, June 2013

2013 ◽  
Vol 18 (27) ◽  
Author(s):  
Å Lundkvist ◽  
J Verner-Carlsson ◽  
A Plyusnina ◽  
L Forslund ◽  
R Feinstein ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Lung Tissue ◽  
Reverse Transcription ◽  
Haemorrhagic Fever ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Specific Antibodies ◽  
Polymerase Chain ◽  
Focus Reduction

We report the first detection of Seoul hantavirus (SEOV) in a pet rat in Sweden. SEOV-specific antibodies were detected in the pet rat blood by focus reduction neutralising test (FRNT), and SEOV RNA in lung tissue was confirmed by reverse transcription-nested polymerase chain reaction (RT-PCR) followed by sequencing. The discovery follows the recent reports of SEOV infected pet rats, as well as associated human cases of severe haemorrhagic fever with renal syndrome (HFRS), in England and Wales.

Validation of a LightCycler-based reverse transcription polymerase chain reaction for the detection of foot-and-mouth disease virus

2003 ◽  
Vol 113 (1) ◽  
pp. 35-41 ◽  
Author(s):  
Peter Moonen ◽  
Jan Boonstra ◽  
Renate Hakze- van der Honing ◽  
Christine Boonstra- Leendertse ◽  
Liesbeth Jacobs ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Disease Virus ◽  
Reverse Transcription ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Chain Reaction ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Polymerase Chain
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