Development of a Quantitative-Competitive Polymerase Chain Reaction Assay for Serotype 1 Marek's Disease Virus

2000 ◽  
Vol 44 (4) ◽  
pp. 770 ◽  
Author(s):  
Sanjay M. Reddy ◽  
Richard L. Witter ◽  
Isabel Gimeno
Keyword(s):  
Polymerase Chain Reaction ◽  
Disease Virus ◽  
Polymerase Chain Reaction Assay ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Marek’S Disease Virus ◽  
Chain Reaction ◽  
Competitive Polymerase Chain Reaction ◽  
Serotype 1 ◽  
Polymerase Chain

scholarly journals Comparative suitability of the feathers, peripheral blood and spleen tissue samples of chickens for the detection of Marek’s disease virus by polymerase chain reaction

2019 ◽  
Vol 35 (1-2) ◽  
pp. 1-6
Author(s):  
MW Rahman ◽  
M Nooruzzaman ◽  
US Suma ◽  
EH Chowdhury ◽  
MR Islam
Keyword(s):  
Polymerase Chain Reaction ◽  
Disease Virus ◽  
Peripheral Blood ◽  
Buffy Coat ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Chain Reaction ◽  
Polymerase Chain

A total of 24 clinical specimens (10 feathers, 8 peripheral bloods and 6 spleens) were collected from 10 suspected outbreaks of Marek’s disease (MD) from Mymensingh, Tangail, Gazipur and Pabna districts of Bangladesh. A polymerase chain reaction (PCR) protocol originally described by Silva (1992) was adopted to detect Marek’s disease virus (MDV) genome in these specimens. All the tested peripheral blood buffy coat samples (100%) were positive for MDV in PCR, while 70% of feather samples and 66.6% of spleen samples were positive. A band of 317 bp size was found in all positive samples. A few samples also yielded additional bands of 185 bp size and/or multiple bands of larger than 317 bp size, indicating the presence of both virulent MDV and the vaccine virus. The study suggests that peripheral blood and feathers from live birds, and feathers from dead birds are the samples of choice for the detection of MDV by PCR. The Bangladesh Veterinarian (2018) 35(1&2): 1-6

scholarly journals Development of a Nested Polymerase Chain Reaction Method to Detect Oncogenic Marek's Disease Virus from Feather Tips

2007 ◽  
Vol 19 (5) ◽  
pp. 471-478 ◽  
Author(s):  
Shiro Murata ◽  
Kyung-Soo Chang ◽  
Sung-Il Lee ◽  
Satoru Konnai ◽  
Misao Onuma ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Disease Virus ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Marek’S Disease Virus ◽  
Chain Reaction ◽  
Nested Polymerase Chain Reaction ◽  
Polymerase Chain ◽  
Highly Virulent ◽  
Meq Gene

For the easy survey of Marek's disease virus (MDV), feather tip–derived DNA from MDV-infected chickens can be used because feather tips are easy to collect and feather follicle epithelium is known to be the only site of productive replication of cell-free MDV. To develop a diagnostic method to differentiate highly virulent strains of MDV from the attenuated MDV vaccine strain, CVI988, which is widely used, nested polymerase chain reaction (PCR) was performed to detect a segment of the meq gene in feather tip samples of chickens experimentally infected with MDV. In chickens infected with Md5, a strain of oncogenic MDV, the meq gene was consistently detected, whereas the L- meq gene, in which a 180–base pair (180-bp) sequence is inserted into the meq gene, was detected in CVI988-infected chickens. Moreover, the meq gene was mainly detected even in chickens co-infected with both Md5 and CVI988. These results suggest that this method is appropriate for the surveillance of the highly virulent MDV infection in the field.

Differentiation of Oncogenic and Nononcogenic Strains of Marek's Disease Virus Type 1 by Using Polymerase Chain Reaction DNA Amplification

1992 ◽  
Vol 36 (3) ◽  
pp. 637 ◽  
Author(s):  
Geng-Sheng Zhu ◽  
Tomoko Ojima ◽  
Takashi Hironaka ◽  
Takeshi Ihara ◽  
Noriko Mizukoshi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Disease Virus ◽  
Virus Type ◽  
Dna Amplification ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Chain Reaction ◽  
Disease Virus Type ◽  
Polymerase Chain
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