Influence of varying nitrogen sources on polysaccharide production by Aureobasidium pullulans in batch culture

1990 ◽  
Vol 32 (6) ◽  
pp. 637-644 ◽  
Author(s):  
Desmond P. F. Auer ◽  
Robert J. Seviour
Keyword(s):  
Batch Culture ◽  
Aureobasidium Pullulans ◽  
Nitrogen Sources ◽  
Polysaccharide Production

Morphogenesis and adhesion of Aureobasidium pullulans

1994 ◽  
Vol 40 (1) ◽  
pp. 6-17 ◽  
Author(s):  
John H. Andrews ◽  
Robin F. Harris ◽  
Russell N. Spear ◽  
Gee W. Lau ◽  
Erik V. Nordheim
Keyword(s):  
Batch Culture ◽  
Aureobasidium Pullulans ◽  
Cell Attachment ◽  
Extracellular Polysaccharide ◽  
Quantitative Detection ◽  
Total Biomass ◽  
Dimorphic Fungus ◽  
Solid Media ◽  
Carbon And Nitrogen Content ◽  
Cellulose Membranes

Two strains of the dimorphic fungus Aureobasidium pullulans were grown in liquid and on solid media varying in carbon and nitrogen content, and on leaf surfaces. Hyphae were observed in all systems but comprised a very low proportion (often below quantitative detection) of the total biomass. In liquid media, hyphae were found sparsely and only in the wash-zone on walls of the culture flasks. Yeast phase growth (blastospores) occurred in pH-buffered media that were nutrient balanced, or continuously carbon-limited (fed-batch culture), or carbon-exhausted (batch culture). Blastospores exposed to conditions with limited nitrogen but sufficient organic carbon, or to acidified media, converted to swollen cells and chlamydospores. The latter morphotypes accumulated carbon internally as lipid granules, and then externally as capsular and soluble extracellular polysaccharide. They were cohesive and also adhered more strongly to cellulose membranes overlying agar media or to leaves than did blastospores. Pullulanase treatment diminished the capsules, cohesion, and adhesion. Addition of soluble extracellular polysaccharide to blastospores enhanced their adhesion to leaves. We conclude that extracellular polysaccharide can play a role in adhesion of A. pullulans.Key words: phylloplane, leaf surface, epiphytic growth, extracellular polysaccharide, cell attachment.

Peroxidase production by carbon and nitrogen sources fed-batch culture of Arthromyces ramosus

1994 ◽  
Vol 16 (6) ◽  
pp. 575-580
Author(s):  
Hideo Tsujimura ◽  
Masamitsu Takaya ◽  
Kenji Katano ◽  
Nobuya Matsumoto ◽  
Yong Soo Park ◽  
...  
Keyword(s):  
Batch Culture ◽  
Nitrogen Sources ◽  
Fed Batch ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Fed Batch Culture

The mechanism of improved pullulan production by nitrogen limitation in batch culture of Aureobasidium pullulans

2015 ◽  
Vol 127 ◽  
pp. 325-331 ◽  
Author(s):  
Dahui Wang ◽  
Feifei Chen ◽  
Gongyuan Wei ◽  
Min Jiang ◽  
Mingsheng Dong
Keyword(s):  
Batch Culture ◽  
Aureobasidium Pullulans ◽  
Nitrogen Limitation

Extracellular cellulase production by tropical isolates of Aureobasidium pullulans

2005 ◽  
Vol 51 (9) ◽  
pp. 773-776 ◽  
Author(s):  
T Kudanga ◽  
E Mwenje
Keyword(s):  
Carboxymethyl Cellulose ◽  
Cell Walls ◽  
Aureobasidium Pullulans ◽  
Plant Cell Wall ◽  
Synthetic Medium ◽  
Cellulase Activity ◽  
Nitrogen Sources ◽  
Cellulase Production ◽  
Cellulolytic Activity ◽  
Specific Activities

Cellulase production by Aureobasidium pullulans from the temperate regions has remained speculative, with most studies reporting no activity at all. In the current study, tropical isolates from diverse sources were screened for cellulase production. Isolates were grown on a synthetic medium containing cell walls of Msasa tree (Brachystegia sp.) as the sole carbon source, and their cellulolytic activities were measured using carboxymethyl cellulose and α-cellulose as substrates. All isolates studied produced carboxymethyl cellulase (endoglucanase) and alpha-cellulase (exoglucanase) activity. Endoglucanase-specific activities of ten selected isolates ranged from 2.375 to 12.884 µmol glucose·(mg protein)–1·h–1, while activities on α-cellulose (exoglucanase activity) ranged from 0.293 to 22.442 µmol glucose·(mg protein)–1·day–1. Carboxymethyl cellulose induced the highest cellulase activity in the selected isolates, while the isolates showed variable responses to nitrogen sources. The current study indicates that some isolates of A. pullulans of tropical origin produce significant extracellular cellulolytic activity and that crude cell walls may be good inducers of cellulolytic activity in A. pullulans.Key words: Aureobasidium pullulans, plant cell wall, cellulases, endoglucanase, exoglucanase.

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