High cell density perfusion culture of hybridoma cells recycling high molecular weight components

1988 ◽  
Vol 1 (2) ◽  
pp. 171-178 ◽  
Author(s):  
Yoshiharu Takazawa ◽  
Michiyuki Tokashiki ◽  
Kimihiko Hamamoto ◽  
Hiroki Murakami
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Cell Density ◽  
High Cell Density ◽  
Perfusion Culture ◽  
Hybridoma Cells ◽  
High Cell

scholarly journals High Cell Density Perfusion Culture has a Maintained Exoproteome and Metabolome

2018 ◽  
Vol 13 (10) ◽  
pp. 1800036 ◽  
Author(s):  
Leila Zamani ◽  
Magnus Lundqvist ◽  
Ye Zhang ◽  
Magnus Aberg ◽  
Fredrik Edfors ◽  
...  
Keyword(s):  
Cell Density ◽  
High Cell Density ◽  
Perfusion Culture ◽  
High Cell

Production of human-mouse chimeric antibody by high cell density perfusion culture

1989 ◽  
Vol 2 (2) ◽  
pp. 95-101 ◽  
Author(s):  
Yoshiharu Takazawa ◽  
Michiyuki Tokashiki
Keyword(s):  
Cell Density ◽  
High Cell Density ◽  
Perfusion Culture ◽  
Chimeric Antibody ◽  
High Cell

Degradation of benzo[a]pyrene, dibenz[a,h]anthracene and coronene by burkholderia cepacia

1997 ◽  
Vol 36 (10) ◽  
pp. 45-51 ◽  
Author(s):  
Albert L. Juhasz ◽  
Margaret L. Britz ◽  
Grant A. Stanley
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Burkholderia Cepacia ◽  
High Cell Density ◽  
Complex Mixture ◽  
Sole Source ◽  
High Cell ◽  
Cell Numbers ◽  
Plant Soil ◽  
High Molecular Weight Pahs

A Burkholderia cepacia strain (VUN 10,001) isolated from manufacturing gas plant soil was selected for its ability to grow on and degrade pyrene as a sole source of carbon and energy. VUN 10,001 was able to grow on fluorene, phenanthrene benz[a]anthracene, fluoranthene and pyrene, but not benzo[a]pyrene and dibenz[a,h]anthracene when tested using inocula with low cell numbers. However, a small decrease in the concentrations of these latter compounds was observed. When media were inoculated at a high cell density, VUN 10,001 degraded the high molecular weight PAHs benzo[a]pyrene, dibenz[a,h]anthracene and coronene when these were present as individual substrates or as components of a complex mixture. Degradation of the high molecular weight PAHs was found to increase in the presence of the lower molecular weight compounds.

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