Model Simulation and Analysis of Perfusion Culture of Mammalian Cells at High Cell Density

1999 ◽  
Vol 15 (3) ◽  
pp. 373-382 ◽  
Author(s):  
A.-P. Zeng ◽  
W.-D. Deckwer
Keyword(s):  
Cell Density ◽  
Mammalian Cells ◽  
High Cell Density ◽  
Model Simulation ◽  
Perfusion Culture ◽  
High Cell

scholarly journals High Cell Density Perfusion Culture has a Maintained Exoproteome and Metabolome

2018 ◽  
Vol 13 (10) ◽  
pp. 1800036 ◽  
Author(s):  
Leila Zamani ◽  
Magnus Lundqvist ◽  
Ye Zhang ◽  
Magnus Aberg ◽  
Fredrik Edfors ◽  
...  
Keyword(s):  
Cell Density ◽  
High Cell Density ◽  
Perfusion Culture ◽  
High Cell

Production of human-mouse chimeric antibody by high cell density perfusion culture

1989 ◽  
Vol 2 (2) ◽  
pp. 95-101 ◽  
Author(s):  
Yoshiharu Takazawa ◽  
Michiyuki Tokashiki
Keyword(s):  
Cell Density ◽  
High Cell Density ◽  
Perfusion Culture ◽  
Chimeric Antibody ◽  
High Cell

Metabolic Flux Analysis of Mammalian Cells in High Cell Density Perfusion Cultures Targetting Real-Time Application

2001 ◽  
Vol 34 (5) ◽  
pp. 137-142
Author(s):  
Richard Biener ◽  
Chetan Goudar ◽  
Renato Meneses ◽  
Maria Ng ◽  
Chun Zhang ◽  
...  
Keyword(s):  
Real Time ◽  
Cell Density ◽  
Metabolic Flux Analysis ◽  
Mammalian Cells ◽  
Metabolic Flux ◽  
High Cell Density ◽  
Flux Analysis ◽  
High Cell ◽  
Perfusion Cultures ◽  
Real Time Application

scholarly journals Production of a self-activating CBM-factor X fusion protein in a stable transformed Sf9 insect cell line using high cell density perfusion culture

2004 ◽  
Vol 44 (3) ◽  
pp. 93-102 ◽  
Author(s):  
Volker M. Gorenflo ◽  
Tom A. Pfeifer ◽  
Gary Lesnicki ◽  
Emily M. Kwan ◽  
Thomas A. Grigliatti ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Cell Line ◽  
Cell Density ◽  
Insect Cell ◽  
High Cell Density ◽  
Insect Cell Line ◽  
Perfusion Culture ◽  
Factor X ◽  
High Cell

scholarly journals Pichia pastoris: SEL RAGI UNTUK PRODUKSI PROTEIN REKOMBINAN

2018 ◽  
Vol 17 (2) ◽  
Author(s):  
Neng Herawati ◽  
Arizah Kusumawati ◽  
Adi Santoso
Keyword(s):  
Pichia Pastoris ◽  
Recombinant Protein ◽  
Cell Density ◽  
Mammalian Cells ◽  
Recombinant Protein Production ◽  
Protein Production ◽  
High Cell Density ◽  
Expression System ◽  
Scale Up ◽  
High Cell

Pichia pastoris is a group of methylotropic yeast known as a host of expression and protein production which is widely used for biopharmaceutical-based drug production. This yeast can grow fast with a high cell density. Its genetic stability, high cell density, and stress resistance make the development process and scale-up of P. pastoris can increase to a scale of 200,000 liters of culture. In contrast to the expensive and complex development of recombinant protein production in mammalian cells, the development of production in P. pastoris is relatively simple and cheaper. The advantage of P. pastoris as an expression system is that it is able to use methanol as a carbon source by inducing the expression of alcohol oxidase oxidase (AOX) enzyme. Promoter used by this enzyme is also used as a strong promoter for the expression of proteins that we want. Unlike in bacterial and mammalian systems, recombinant protein production in Pichia cells is not contaminated with endotoxins or viruses so it is safer and simplifies the downstream processes in bioproduction. The level of endogenous protein in the low supernatant allows Pichia to cultivate with a high volumetric productivity, therefore the process of protein production becomes very economical. This review provides an overview of several things that must be considered in utilizing P. pastoris as an expression system including the selection of vectors, strains, vector integration mechanisms into the genome, glycosylation processes, and applications in industry.

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