Genotypic and exogenous factors affecting shoot regeneration from anther callus of linseed (Linum usitatissimum L.)

Euphytica ◽  
1991 ◽  
Vol 58 (2) ◽  
pp. 157-164 ◽  
Author(s):  
Karin Nichterlein ◽  
Helga Umbach ◽  
Wolfgang Friedt
Keyword(s):  
Shoot Regeneration ◽  
Linum Usitatissimum ◽  
Factors Affecting ◽  
Linum Usitatissimum L ◽  
Exogenous Factors

scholarly journals The effect of magnetic field strength on shoot regeneration and Agrobacterium tumefaciens-mediated gene transfer in flax (Linum usitatissimum L.)

2019 ◽  
Vol 55 (No. 1) ◽  
pp. 20-27
Author(s):  
Murat Aycan ◽  
Ramazan Beyaz ◽  
Anzel Bahadir ◽  
Mustafa Yildiz
Keyword(s):  
Magnetic Field ◽  
Agrobacterium Tumefaciens ◽  
Gene Transfer ◽  
Shoot Regeneration ◽  
Linum Usitatissimum ◽  
Naphthaleneacetic Acid ◽  
Nptii Gene ◽  
Ms Medium ◽  
Generator System ◽  
Linum Usitatissimum L

This study was conducted to determine the effects of magnetic field (MF) strength on shoot regeneration and Agrobacterium tumefaciens-mediated gene transfer in flax (Linum usitatissimum L.). Seeds of flax cv. Madaras were exposed to different MF strengths (0 – control, 75, 150, and 300 millitesla (mT)) for 24 h by using an electromagnetic generator system fabricated in laboratory conditions. After sterilization, seeds were germinated on MS (Murashige and Skoog) medium in Magenta vessels. Hypocotyl explants excised from 7-days-old seedlings were used for regeneration. GV2260 strain of Agrobacterium tumefaciens was used in transformation studies. Inoculated hypocotyls were cultured on MS medium containing 1 mg/l BAP (6-benzylaminopurine) and 0.02 mg/l NAA (naphthaleneacetic acid) for 2 days by co-cultivation. Then, they were transferred to MS medium containing the same growth regulators, 100 mg/l kanamycin and 500 mg/l Duocid for selection. The presence of the nptII gene was verified by PCR (polymerase chain reaction) analysis in putative transgenic plants. The highest results with respect to shoot regeneration and transformation frequency were obtained from treatments of 75 mT MF strength.  

The correlative inhibition of bud and shoot growth in flax (Linum usitatissimum). Some factors affecting the pattern and degree of inhibition

1975 ◽  
Vol 53 (4) ◽  
pp. 390-402 ◽  
Author(s):  
Gordon I. McIntyre
Keyword(s):  
Shoot Apex ◽  
Linum Usitatissimum ◽  
Nitrogen Nutrition ◽  
Growth Potential ◽  
Sand Culture ◽  
Factors Affecting ◽  
Linum Usitatissimum L ◽  
N Supply ◽  
Lateral Buds ◽  
Correlative Inhibition

When flax (Linum usitatissimum L.) seedlings were grown in sand culture under controlled conditions, growth of the lateral buds and their release from apical dominance showed a positive correlation with the N supply. The response of the buds was related to their position on the shoot and a well-defined gradient of bud growth potential was apparent at all N levels. Removal of the basal buds, which have the highest growth potential, induced the outgrowth of buds at higher nodes and increased the growth of the main shoot apex. Similar effects were produced by increasing the N supply to the intact plant.Providing N only as nitrate inhibited apical growth and caused severe leaf necrosis. These effects were due to zinc deficiency and could be prevented or significantly reduced not only by increasing the zinc supply but also by reducing the N level, providing some NH4-N, reducing the light intensity, or increasing the depth of planting. They could also be prevented by removal of the lateral buds at the basal nodes, thus indicating that the shoot apex and lateral buds may compete for the limited zinc supply. The obviously complex relationship between zinc and nitrogen nutrition in this species requires further investigation.

IN VITRO REGENERATION OF SHOOTS ON STEM EXPLANTS OF HAPLOID AND DIPLOID FLAX (LINUM USITATISSIMUM)

1977 ◽  
Vol 19 (1) ◽  
pp. 177-186 ◽  
Author(s):  
Beatrice E. Murray ◽  
R. J. Handyside ◽  
W. A. Keller
Keyword(s):  
Chromosome Number ◽  
Shoot Regeneration ◽  
Linum Usitatissimum ◽  
In Vitro Regeneration ◽  
Chromosome Complement ◽  
Stem Explants ◽  
Linum Usitatissimum L ◽  
Napthaleneacetic Acid ◽  
Lower Temperature

Shoots were regenerated from stem explants of haploid (2n = x = 15) and diploid (2n = 2x = 30) flax (Linum usitatissimum L. cv. Rocket 4) on a modified Murashige and Skoog (1962) medium supplemented with benzyladenine and napthaleneacetic acid. Segments of 15 mm lengths, cultured from donor plants at early flowering, regenerated 6.1 (mean) shoots per mm which were epidermal in origin and retained the chromosome number of the donor. The rate and frequency of shoot regeneration was greater at 30 °C as compared to 25 °C. Callus originated from the cut ends of explants and was more vigorous at the lower temperature. Shoots of callus origin were infrequent and had the doubled chromosome complement (2n = 2x = 30 and 2n = 4x = 60).

Hepatoprotective activity and standardization of the bioactive lignan fraction of Linum usitatissimum L.

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
H El-Askary ◽  
S El Zalabani ◽  
RS El-Din ◽  
MY Issa ◽  
RR Hegazy ◽  
...  
Keyword(s):  
Linum Usitatissimum ◽  
Hepatoprotective Activity ◽  
Linum Usitatissimum L

ОСОБЕННОСТИ СЕЛЕКЦИИ И ПЕРСПЕКТИВЫ ПРИМЕНЕНИЯ МОЛЕКУЛЯРНО-ГЕНЕТИЧЕСКИХ МЕТОДОВ В ГЕНЕТИКО- СЕЛЕКЦИОННЫХ ИССЛЕДОВАНИЯХ ЛЬНА (Linum usitatissimum L.) (обзор)

2016 ◽  
Vol 51 (5) ◽  
pp. 602-616 ◽  
Author(s):  
И.В. УЩАПОВСКИЙ И.В. УЩАПОВСКИЙ ◽  
◽  
В.А. ЛЕМЕШ В.А. ЛЕМЕШ ◽  
М.В. БОГДАНОВА М.В. БОГДАНОВА ◽  
Е.В. ГУЗЕНКО Е.В. ГУЗЕНКО ◽  
...  
Keyword(s):  
Linum Usitatissimum ◽  
Linum Usitatissimum L
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