IN VITRO REGENERATION OF SHOOTS ON STEM EXPLANTS OF HAPLOID AND DIPLOID FLAX (LINUM USITATISSIMUM)

1977 ◽  
Vol 19 (1) ◽  
pp. 177-186 ◽  
Author(s):  
Beatrice E. Murray ◽  
R. J. Handyside ◽  
W. A. Keller
Keyword(s):  
Chromosome Number ◽  
Shoot Regeneration ◽  
Linum Usitatissimum ◽  
In Vitro Regeneration ◽  
Chromosome Complement ◽  
Stem Explants ◽  
Linum Usitatissimum L ◽  
Napthaleneacetic Acid ◽  
Lower Temperature

Shoots were regenerated from stem explants of haploid (2n = x = 15) and diploid (2n = 2x = 30) flax (Linum usitatissimum L. cv. Rocket 4) on a modified Murashige and Skoog (1962) medium supplemented with benzyladenine and napthaleneacetic acid. Segments of 15 mm lengths, cultured from donor plants at early flowering, regenerated 6.1 (mean) shoots per mm which were epidermal in origin and retained the chromosome number of the donor. The rate and frequency of shoot regeneration was greater at 30 °C as compared to 25 °C. Callus originated from the cut ends of explants and was more vigorous at the lower temperature. Shoots of callus origin were infrequent and had the doubled chromosome complement (2n = 2x = 30 and 2n = 4x = 60).

scholarly journals In vitro regeneration and polyploidy induction in Pelargonium × hortorum L. H. Bailey

2009 ◽  
Vol 57 (5) ◽  
pp. 305-312
Author(s):  
Hana Vejsadová ◽  
Petra Kuchtová-Jadrná
Keyword(s):  
Flow Cytometry ◽  
Plant Growth ◽  
Shoot Regeneration ◽  
In Vitro Regeneration ◽  
Infiltration Method

The objective of this work was to induce in vitro shoot regeneration as influenced by plant growth re­gu­la­tors and ascertain an effective method of polyploidy induction using colchicine and oryzalin in two diploid cultivars ‘Gizela’ and ‘Black Velvet Scarlet F1’ of Pelargonium × hortorum L. H. Bailey. In both cultivars, benzyladenine (BA) significantly improved shoot regeneration compared to zeatin. The infiltration and the dip methods of explant treatment were used for polyploidy induction. Regenerants were analyzed using the flow cytometry (FCM). In ‘Gizela’ and ‘Black Velvet Scarlet F1’, 10 tetraploids on the level of 2n = 4x were found by the infiltration method. The tetraploidy was determined by the dip method in 4 shoots of ‘Gizela’ and 11 tetraploids were detected in ‘Black Velvet Scarlet F1’.

scholarly journals In vitro Regeneration and Rapid Multiplication of Dendrobium bensoniae, an Indigenous Ornamental Orchid

2017 ◽  
Vol 14 (2) ◽  
pp. 24-31 ◽  
Author(s):  
S S Riva ◽  
A Islam ◽  
M E Hoque
Keyword(s):  
Shoot Regeneration ◽  
In Vitro Regeneration ◽  
Ms Medium ◽  
Shoot Induction ◽  
Rapid Multiplication ◽  
Number Of Shoots

An experiment was conducted on in vitro regeneration and multiplication of Dendrobium bensoniae. Different concentrations of BA and IBA alone or combination of both hormones were used as treatment for regeneration.  It was revealed that shoot regeneration from node was the best at 2.0 mg/l BA supplemented to MS medium. It gave better responses than all other concentrations and combinations of BA and BA+IBA, used in the present study. The highest number of shoots and leaves were found when 1.0 mg/l BA with 1.5 mg/l IBA was supplemented into MS medium.  For rooting, 0.5 mg/l BA with 1.0 mg/l IBA was found to be the most effective. The well-rooted plantlets were successfully acclimatized under 70-80% humidity and planted in pots and transferred to the shade house for establishment. Around 85% of plantlets survived in the field. From the present result, it may be recommended that MS medium supplemented with 2.0 mg/l BA may be used for rapid shoot induction and regeneration of D. bensoniae.The Agriculturists 2016; 14(2) 24-31

scholarly journals Potential of Launea taraxacifolia (Willd) Amin Ex. C. Jeffrey for in Vitro Regeneration

2011 ◽  
Vol 3 (3) ◽  
pp. 93-96
Author(s):  
Ayobola M.A. SAKPERE ◽  
Ejeoghene R. AYISIRE ◽  
Olufemi I. ABIOYE
Keyword(s):  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Dichlorophenoxyacetic Acid ◽  
Stem Explants ◽  
Ms Medium ◽  
First Report ◽  
Full Strength

This study investigated the potential of Launea taraxacifolia for in vitro regeneration. Stem and leaf explants were inoculated on full strength Murashige and Skoog (MS) medium supplemented with varying concentrations of 2, 4-dichlorophenoxyacetic acid (2,4-D). Leaf explants responded to all concentrations of 2,4-D used while stem explants responded to only two of the 2, 4-D concentrations suggesting that leaf explants might be a better source of explants. Leaf explants generated shoots on medium supplemented with 0.5 mg/l kinetin and 0.1 mg/l 2, 4-D. This study is the first report on in vitro regeneration of Launea taraxacifolia.

scholarly journals Factors Affecting the Regeneration, via Organogenesis, and the Selection of Transgenic Calli in the Peach Rootstock Hansen 536 (Prunus persica × Prunus amygdalus) to Express an RNAi Construct against PPV Virus

Plants ◽  
2019 ◽  
Vol 8 (6) ◽  
pp. 178 ◽  
Author(s):  
Sabbadini ◽  
Ricci ◽  
Limera ◽  
Baldoni ◽  
Capriotti ◽  
...  
Keyword(s):  
Shoot Regeneration ◽  
Prunus Persica ◽  
Fluorescent Protein ◽  
In Vitro Regeneration ◽  
Naphthaleneacetic Acid ◽  
Prunus Amygdalus ◽  
Indirect Organogenesis ◽  
Egfp Gene ◽  
Rnai Construct

Prunus spp. is one of the most recalcitrant fruit tree species in terms of in vitro regeneration and transformation, mostly when mature tissues are used as explants. The present study describes the in vitro regeneration via indirect organogenesis, and Agrobacterium tumefaciens-mediated transformation of the peach rootstock Hansen 536 (Prunus persica × Prunus amygdalus) through the use of meristematic bulks (MBs) as starting explants. Efficient adventitious shoot regeneration was obtained when Hansen 536 MBs were cultured on an optimized medium consisting of modified McCown Woody Plant medium (WPM) enriched with 4.4 M 6-Benzyladenine (BA), 0.1 M 1-Naphthaleneacetic acid (NAA) and 6.0 g L−1 plant agar S1000 (B&V). MB slices were used later as starting explants for Agrobacterium-mediated transformation to introduce an RNAi construct “ihp35S-PPV194” against PPV virus. Transgenic events were identified by both green fluorescent protein (GFP) screening and kanamycin selection at different concentrations (0, 17 or 42 M). GFP-fluorescent proliferating callus lines were selected and confirmed to stably express the ihp35S-PPV194::eGFP gene construct by molecular analysis. Although shoot regeneration from these transgenic calli has not been obtained yet, this represents one of the few examples of successful attempts in peach genetic transformation from somatic tissues, and also serves as a useful in vitro system for future gene functional analysis in peach.

Large scale screening of heavy metal tolerance in flax/linseed (Linum usitatissimum L.) tested in vitro

2010 ◽  
Vol 32 (3) ◽  
pp. 527-533 ◽  
Author(s):  
Iva Smykalova ◽  
Miroslava Vrbova ◽  
Eva Tejklova ◽  
Martina Vetrovcova ◽  
Miroslav Griga
Keyword(s):  
Heavy Metal ◽  
Linum Usitatissimum ◽  
Large Scale ◽  
Metal Tolerance ◽  
Heavy Metal Tolerance ◽  
Linum Usitatissimum L ◽  
Large Scale Screening
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