The effect of pedicel length on lateral kernel weight in two-six rowed near isogenic lines of barley (Hordeum vulgare L.)

Euphytica ◽  
1986 ◽  
Vol 35 (2) ◽  
pp. 363-368 ◽  
Author(s):  
K. M. Gilbertson ◽  
E. A. Hockett
Keyword(s):  
Hordeum Vulgare ◽  
Kernel Weight ◽  
Near Isogenic Lines ◽  
Hordeum Vulgare L ◽  
Isogenic Lines ◽  
Pedicel Length

AFLP targeting of the 1-cM region conferring the vrs1 gene for six-rowed spike in barley, Hordeum vulgare L.

Genome ◽  
2004 ◽  
Vol 47 (6) ◽  
pp. 1122-1129 ◽  
Author(s):  
Congfen He ◽  
Badraldin Ebrahim Sayed-Tabatabaei ◽  
Takao Komatsuda
Keyword(s):  
Hordeum Vulgare ◽  
Fragment Length ◽  
Positional Cloning ◽  
Aflp Markers ◽  
Near Isogenic Lines ◽  
Hordeum Vulgare L ◽  
Plant Materials ◽  
Multiple Alleles ◽  
Isogenic Lines ◽  
Sequence Tagged Sites

Spike morphology is a key characteristic in the study of barley domestication, yield, and use. Multiple alleles at the vrs1 locus control the development and fertility of the lateral spikelets of barley. We developed five amplified fragment length polymorphism (AFLP) markers tightly linked to the vrs1 locus using well-characterized near-isogenic lines as plant materials. The AFLP markers were integrated into three different maps, in which 'Azumamugi' was used as the maternal parent. Of the three maps, Hordeum vulgare L. 'Azumamugi' × H. vulgare 'Golden Promise' showed recombination of the AFLP markers and the vrs1 locus (closest, 0.05 cM), providing the best mapping population for positional cloning of alleles at the vrs1 locus. Conversion of AFLP bands into polymorphic sequence-tagged sites (STSs) is necessary for further high-throughput genotype scoring and for bacterial artificial chromosome (BAC) library screening. We cloned and sequenced the five AFLP bands and synthesized primer pairs. PCR amplification generated DNAs of the same size from all four parental lines for each marker. Restriction endonuclease treatment of e40m36-1110/AccIII, e34m13-260/Psp1406I, e52m32-270/FokI, and e31m26-520/MnlI revealed fragment length polymorphisms between 'Azumamugi' and all the two-rowed parents. Allelism between the AFLPs and corresponding STS markers was confirmed genetically, indicating the usefulness of the STSs as genetic markers.Key words: positional cloning, codominance, near-isogenic lines, high-resolution maps, STSs.

Transfer and mapping of the shoot-differentiation locus Shd1 in barley chromosome 2

Genome ◽  
1995 ◽  
Vol 38 (5) ◽  
pp. 1009-1014 ◽  
Author(s):  
T. Komatsuda ◽  
F. Taguchi-Shiobara ◽  
S. Oka ◽  
F. Takaiwa ◽  
T. Annaka ◽  
...  
Keyword(s):  
Hordeum Vulgare ◽  
Immature Embryo ◽  
Gene Products ◽  
Chromosome 2 ◽  
Near Isogenic Lines ◽  
Hordeum Vulgare L ◽  
Isogenic Lines ◽  
Shoot Differentiation ◽  
Backcross Breeding ◽  
Positive Effect

The locus Shd1, which we previously mapped to the long arm of chromosome 2 of Hordeum vulgare L., controls the differentiation of shoots from immature barley embryo callus. The locus has major effects and its action explains more than 65% of the total genetic variance in the shoot-differentiation rate. The allele of cultivar Kanto Nakate Gold designated Shd1K has a significant positive effect on the shoot-differentiation rate, whereas Shd1A of cultivar Azumamugi does not promote shoot differentiation. To identify gene products and characterize the function of Shd1, a set of near-isogenic lines is essential. In this study we produced BC5F1 plants by repeated backcrossing of 'Azumamugi' to F1 plants ('Azumamugi' × 'Kanto Nakate Gold'). The BC5F1 plants were examined for their RFLP genotype and for the shoot-differentiation ability of immature embryo-derived callus. The results indicated that the Shd1 locus was located in a chromosomal region between MWG2081 and MWG503 that flanks the MWG801, cMWG699, v (ear type), and MWG865 loci. Shd1K from 'Kanto Nakate Gold' functions effectively in the genetic background of 'Azumamugi', an indication that backcross breeding is possible for production of near-isogenic lines that would be very suitable for tissue culture.Key words: Hordeum vulgare L., shoot-differentiation ability, immature embryo callus, backcross breeding, RFLPs.

Classification of cultivated barley (Hordeum vulgare). 1. Historical aspects, and phenetic character analysis of some characters by information theory and by spatial autocorrelation

1986 ◽  
Vol 64 (11) ◽  
pp. 2769-2773
Author(s):  
Bernard B. Baum
Keyword(s):  
Information Theory ◽  
Hordeum Vulgare ◽  
Spatial Autocorrelation ◽  
Theory Model ◽  
Kernel Weight ◽  
Hordeum Vulgare L ◽  
Department Of Agriculture ◽  
Character Analysis ◽  
Barley Cultivars

A brief historical sketch of the classification of barley (Hordeum vulgare L.) cultivars is presented along with reference to key reviews on this subject. Characters, utilized in the comprehensive study on the barley cultivars of North America by Aberg and Wiebe (U.S. Department of Agriculture Technical Bulletin 942), were subjected to a series of phenetic character analyses using an information theory model and a spatial autocorrelation model. The ranking of the 48 characters in order of their importance (for classification and identification purposes) from the character analysis by information theory was compared with the previous rating of characters made by Aberg and Wiebe and was found to differ significantly. Numerous trials of character analysis by spatial autocorrelation using various Minkowski distances, setting various values among three parameters, never yielded results comparable with those obtained by Aberg and Wiebe. Among those trials, a few combinations of values for the three parameters (X, Y, and Z) yielded results comparable with those obtained with character analysis by information theory. Those same combinations of values were found by Estabrook and Gates (Taxon, 33: 13–25) in their study of Banisteriopsis in 1984, where they also developed the method of character analysis by spatial autocorrelation. Kernel weight was found to be the most important character.

COMPARAISON ENTRE DES LIGNEES PEDIGREES ET DES LIGNEES HAPLOIDES DOUBLEES CHEZ L’ORGE (HORDEUM VULGARE L.)

1980 ◽  
Vol 60 (1) ◽  
pp. 79-85 ◽  
Author(s):  
PIERRE TURCOTTE ◽  
C. A. ST-PIERRE ◽  
KEH MING HO
Keyword(s):  
Hordeum Vulgare ◽  
Doubled Haploid ◽  
Doubled Haploids ◽  
Block Design ◽  
Pure Line ◽  
Kernel Weight ◽  
Hordeum Vulgare L ◽  
Line Production ◽  
Doubled Haploid Lines ◽  
Time Required

Pedigree and doubled haploid lines from seven crosses of barley (Hordeum vulgare L.) were compared over 2 years. The lines were tested, in a randomized complete block design, in row plots at Ste-Foy in 1977 and in hill plots at Brawley, California in 1978. There are significant differences between the two methods of pure line production for grain yield, 1000-kernel weight, plant height, resistance to lodging and date of maturity. Furthermore, these significant differences between doubled haploid and pedigree lines seem to be tied to wide crosses. After showing that doubled haploids are superior for resistance to lodging, we conclude that the use of doubled haploid lines must be seriously considered in a barley breeding program on the basis of improved selection efficiency and the short time required to get homozygous lines.

scholarly journals Understanding Mechanisms of Salinity Tolerance in Barley by Proteomic and Biochemical Analysis of Near-Isogenic Lines

2020 ◽  
Vol 21 (4) ◽  
pp. 1516 ◽  
Author(s):  
Juan Zhu ◽  
Yun Fan ◽  
Sergey Shabala ◽  
Chengdao Li ◽  
Chao Lv ◽  
...  
Keyword(s):  
Salinity Tolerance ◽  
Crop Production ◽  
Biochemical Analysis ◽  
Mass Spectrometry Analysis ◽  
Near Isogenic Lines ◽  
Ros Scavenging ◽  
Hordeum Vulgare L ◽  
Two Dimensional Gel Electrophoresis ◽  
Spectrometry Analysis ◽  
Isogenic Lines

Salt stress is one of the major environmental factors impairing crop production. In our previous study, we identified a major QTL for salinity tolerance on chromosome 2H on barley (Hordeum vulgare L.). For further investigation of the mechanisms responsible for this QTL, two pairs of near-isogenic lines (NILs) differing in this QTL were developed. Sensitive NILs (N33 and N53) showed more severe damage after exposure to 300 mM NaCl than tolerant ones (T46 and T66). Both tolerant NILs maintained significantly lower Na+ content in leaves and much higher K+ content in the roots than sensitive lines under salt conditions, thus indicating the presence of a more optimal Na+/K+ ratio in plant tissues. Salinity stress caused significant accumulation of H2O2, MDA, and proline in salinity-sensitive NILs, and a greater enhancement in antioxidant enzymatic activities at one specific time or tissues in tolerant lines. One pair of NILs (N33 and T46) were used for proteomic studies using two-dimensional gel electrophoresis. A total of 53 and 51 differentially expressed proteins were identified through tandem mass spectrometry analysis in the leaves and roots, respectively. Proteins which are associated with photosynthesis, reactive oxygen species (ROS) scavenging, and ATP synthase were found to be specifically upregulated in the tolerant NIL. Proteins identified in this study can serve as a useful resource with which to explore novel candidate genes for salinity tolerance in barley.

Effect of sowing rate on grain yield, kernel weight, and grain protein percentage of barley (Hordeum vulgare L.) in northern New South Wales

1992 ◽  
Vol 32 (4) ◽  
pp. 465 ◽  
Author(s):  
AD Doyle ◽  
RW Kingston
Keyword(s):  
Hordeum Vulgare ◽  
Grain Yield ◽  
Field Experiments ◽  
New South ◽  
New South Wales ◽  
Kernel Weight ◽  
Grain Protein ◽  
Hordeum Vulgare L ◽  
Protein Percentage ◽  
South Wales

The effect of sowing rate (10-110 kg/ha) on the grain yield of barley (Hordeum vulgare L.) was determined from a total of 20 field experiments conducted in northern New South Wales from 1983 to 1986. Effects of sowing rate on kernel weight and grain protein percentage were also determined from 12 experiments conducted in 1985 and 1986. Two barley varieties were tested each year. In all years fallow plus winter rainfall was equal to or greater than average. Grain yield increased with higher sowing rates in most experiments, with the response curve reaching a plateau above 60-70 kg/ha. For 13 of the 40 variety x year combinations, grain yield fell at the highest sowing rates. Only in an experiment where lodging increased substantially with higher sowing rates was there a reduction in yield at a sowing rate of 60 kg/ha. The average sowing rate for which 5 kg grain was produced per kg of seed sown was 63 kg/ha. Grain protein percentage usually fell, and kernel weight invariably fell, with increasing sowing rate. Increasing sowing rates from the normal commercial rate of 35 kg/ha to a rate of 60 kg/ha typically increased grain yields by 100-400 kg/ha, decreased kernel weight by 0.4-2.0 mg, and decreased grain protein by up to 0.5 percentage points. In no case was the grain weight reduced to below malting specifications. It was concluded that sowing rates for barley in northern New South Wales should be increased to about 60 kg/ha.

scholarly journals Barley response to seeding date in central Alberta

2003 ◽  
Vol 83 (2) ◽  
pp. 275-281 ◽  
Author(s):  
P. E. Juskiw ◽  
J. H. Helm
Keyword(s):  
Hordeum Vulgare ◽  
Grain Yield ◽  
Grain Filling ◽  
Kernel Weight ◽  
Seeding Rate ◽  
Hordeum Vulgare L ◽  
Vegetative Period ◽  
Seeding Date ◽  
The Mean ◽  
Grain Filling Period

Seeding date is an important factor influencing productivity of barley (Hordeum vulgare L.). When conditions are conducive to early seeding or result in delayed seeding, producers need to know how cultivars will respond to these seeding situations. In this study, five cultivars (Abee, Harrington, Jackson, Noble and Virden) registered for western Canada were studied for 4 yr (1990 to 1993) when seeded early (late April or early May), in mid-May, in late-May, or late (mid-June) at Lacombe, AB. For all cultivars, early seeding resulted in grain yield advantages of 113 to 134% of the mean site yield, while with late seeding, grain yields were reduced to 54 to 76% of the mean site yield. The reduction in yield was least for Jackson, the earliest maturing cultivar tested. Late seeding reduced the period from sowing to emergence, vegetative period, grain-filling period, time from emergence to physiological maturity, test weight, grain yield, kernel weight, and tillers per plant; and increased plant height and percent thins. Late seeding had no significant effect on phyllochron, stand establishment, scald, lodging, protein content of the grain, kernel number per spike, and spikelet number per spike. Barley responded positively to early seeding in central Alberta, but when seeding was delayed (in this study to mid-June) the early and mid-maturing six-rowed cultivars with short phyllochrons performed better than the two-rowed and late six-rowed cultivars. Key words: Hordeum vulgare L., seeding rate, phenological development, grain quality, grain yield, components

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