Improved Light Microscopy Techniques for Viewing Nannofossils

1973 ◽  
Vol 19 (4) ◽  
pp. 481 ◽  
Author(s):  
David F. Clark
Keyword(s):  
Light Microscopy ◽  
Microscopy Techniques

2013 Microscopy Today Innovation Awards

2013 ◽  
Vol 21 (5) ◽  
pp. 40-45
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Scanning Probe Microscopy ◽  
Scanning Probe ◽  
Probe Microscopy ◽  
Analysis Methods ◽  
Microscopy Analysis ◽  
Ion Microscopy ◽  
Microscopy Techniques

Microscopy Today congratulates the fourth annual group of Innovation Award winners. The ten innovations described below move several microscopy techniques forward: light microscopy, scanning probe microscopy, electron microscopy, ion microscopy, and hybrid microscopy-analysis methods. These innovations will make imaging and analysis more powerful, more flexible, more productive, and easier to accomplish.

Thin-Section Preparation and Transmitted-Light Microscopy

2010 ◽  
pp. 115-135
Keyword(s):  
Light Microscopy ◽  
Polymeric Composites ◽  
Glass Slide ◽  
Transmitted Light ◽  
Fiber Reinforced ◽  
Transmitted Light Microscopy ◽  
Ultrathin Sections ◽  
Microscopy Techniques ◽  
Surface Mounting ◽  
Selection Of

Abstract Transmitted-light methods reveal more details of the morphology of fiber-reinforced polymeric composites than are observable using any other available microscopy techniques. This chapter describes the various aspects relating to the selection and preparation of ultrathin-section specimens of fiber-reinforced polymeric composites for examination by transmitted-light microscopy techniques. The preparation steps covered are a selection of the rough section, preparation of the rough section for preliminary mounting, grinding and polishing the primary-mount first surface, mounting the first surface on a glass slide, and preparing the second surface (top surface). The optimization of microscope conditions and analysis of specimens by microscopy techniques are also covered. In addition, examples of composite ultrathin sections that are analyzed using transmitted-light microscopy contrast methods are shown throughout.

Electron Microscopy Applied to Fine-Needle Aspiration. A Report of Six Cases from Various Sites

1983 ◽  
Vol 69 (5) ◽  
pp. 423-435 ◽  
Author(s):  
Saverio Cinti ◽  
Maurizio Ferretti ◽  
Silvana Amati ◽  
Giancarlo Balercia ◽  
Adalberto Vecchi ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Fine Needle Aspiration ◽  
Needle Aspiration ◽  
Ultrastructural Analysis ◽  
Tissue Structure ◽  
Fine Needle ◽  
Neoplastic Lesions ◽  
Microscopy Techniques

The authors report the results obtained from the application of electron microscopy techniques to the cytology of fine-needle-aspirated samples of neoplastic lesions from various body sites. These results show that the tissue structure, which is usually lost during the squashing necessary for light microscopy cytology, is preserved when the samples are processed for ultrastructural analysis. Electron microscopy also allows a highly detailed study of the cell's inner structures. Thus, when this technique is applied, fine needle-aspirated samples can be regarded as actual microbiopsies. However, because of the high cost of ultrastructural techniques, we suggest that actual analysis be performed only in selected cases, whereas fixation and inclusion for electron microscopy could be done routinely.

scholarly journals APPLICATION OF MICROPLATE BIOFILM ASSAY AND MICROSCOPY TECHNIQUES TO INVESTIGATE ABILITY OF SOME BACTERIAL STRAINS OF ANIMAL ORIGIN TO FORM BIOFILM

2010 ◽  
Vol 3 (1) ◽  
pp. 23-37
Author(s):  
Dubravka Milanov ◽  
Dejan Bugarski ◽  
Jelena Petrović ◽  
Olga Rackov
Keyword(s):  
Stainless Steel ◽  
Light Microscopy ◽  
Bacterial Species ◽  
Animal Origin ◽  
Stainless Steel Surface ◽  
Bacterial Strains ◽  
Simple Method ◽  
In Vitro Investigation ◽  
Microscopy Techniques ◽  
Scanning Electron

The use of some procedures for in vitro investigation of biofilm formation in bacterial species Staphylococcus aureus, Listeria monocytogenes and Pseudomonas aeruginosa isolated from animals is presented in this paper. Biofilms are formed on polystyrene and stainless steel surfaces. On polystyrene surface S. aureus and L. monocytogenes were examined by microplate biofilm assay and light microscopy. Biofilms of L. monocytogenes, S. aureus and P. aeruginosa, formed on stainless steel surface, were examined by scanning electron microscopy. The application of these methods can help distinguish between the bacteria isolates that don’t form and the one that form biofilm. Microplate biofilm assay proved to be a simple method suitable for examining a large number of isolates of the same or different bacteria species, particularly if used prior to other investigation techniques that require more complex procedure. The advantages of the test, among the others, is quantification of the obtained results, whereas a principal drawback implicates the impossibility of detecting extracellular substance. The microscopy techniques have provided a direct insight in the structures formed by the investigated strains on the used substrates, with some limitations. They are related to the poor resolution features and two-dimensional image obtained by light microscopy, as well as deformation of three-dimensional biofilm structures in the preparation for scanning electron microskopy.

scholarly journals Imaging Lipid Bodies Within Leukocytes with Different Light Microscopy Techniques

2010 ◽  
pp. 149-161 ◽  
Author(s):  
Rossana C.N. Melo ◽  
Heloisa D’Ávila ◽  
Patricia T. Bozza ◽  
Peter F. Weller
Keyword(s):  
Light Microscopy ◽  
Lipid Bodies ◽  
Microscopy Techniques
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