scholarly journals In SituLocalization of the Putative Movement Protein (pr17) from Potato Leafroll Luteovirus (PLRV) in Infected and Transgenic Potato Plants

Virology ◽  
1997 ◽  
Vol 235 (2) ◽  
pp. 311-322 ◽  
Author(s):  
J. Schmitz ◽  
C. Stussi-Garaud ◽  
E. Tacke ◽  
D. Prüfer ◽  
W. Rohde ◽  
...  
Keyword(s):  
Movement Protein ◽  
Transgenic Potato ◽  
Potato Plants ◽  
Transgenic Potato Plants ◽  
Putative Movement Protein ◽  
Potato Leafroll Luteovirus

scholarly journals Expression of the Potato Leafroll Virus ORF0 Induces Viral-Disease-like Symptoms in Transgenic Potato Plants

1997 ◽  
Vol 10 (2) ◽  
pp. 153-159 ◽  
Author(s):  
Frank van der Wilk ◽  
Petra Houterman ◽  
Jos Molthoff ◽  
Fabienne Hans ◽  
Ben Dekker ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Transgenic Potato ◽  
Viral Disease ◽  
Host Recognition ◽  
Reading Frame ◽  
Potato Plants ◽  
Transgenic Potato Plants ◽  
Potato Leafroll Luteovirus ◽  
Leafroll Virus

The role of the open reading frame 0 (ORF0) of luteoviruses in the viral infection cycle has not been resolved, although the translation product (p28) of this ORF has been suggested to play a role in host recognition. To investigate the function of the potato leafroll luteovirus (PLRV) p28 protein, transgenic potato plants were produced containing the ORF0. In the lines in which the ORF0 transcripts could be detected by Northern (RNA) analysis, the plants displayed an altered phenotype resembling virus-infected plants. A positive correlation was observed between levels of accumulation of the transgenic transcripts and severity of the phenotypic aberrations observed. In contrast, potato plants transformed with a modified, untranslatable ORF0 sequence were phenotypically indistinguishable from wild-type control plants. These results suggest that the p28 protein is involved in viral symptom expression. Southern blot analysis showed that the transgenic plants that accumulated low levels of ORF0 transcripts detectable only by reverse transcription-polymerase chain reaction, contained methylated ORF0 DNA sequences, indicating down-regulation of the transgene provoked by the putatively unfavorable effects p28 causes in the plant cell.

Expression of the potato leafroll luteovirus coat protein gene in transgenic potato plants inhibits viral infection

1991 ◽  
Vol 17 (3) ◽  
pp. 431-439 ◽  
Author(s):  
Frank van der Wilk ◽  
Dinie Posthumus-Lutke Willink ◽  
Marianne J. Huisman ◽  
Harm Huttinga ◽  
Rob Goldbach
Keyword(s):  
Coat Protein ◽  
Viral Infection ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Transgenic Potato ◽  
Potato Plants ◽  
Transgenic Potato Plants ◽  
Potato Leafroll Luteovirus

Sucrose synthase activity does not restrict glycolysis in roots of transgenic potato plants under hypoxic conditions

Planta ◽  
1999 ◽  
Vol 210 (1) ◽  
pp. 41-49 ◽  
Author(s):  
Sophia Biemelt ◽  
Mohammad Reza Hajirezaei ◽  
Michael Melzer ◽  
Gerd Albrecht ◽  
Uwe Sonnewald
Keyword(s):  
Sucrose Synthase ◽  
Transgenic Potato ◽  
Potato Plants ◽  
Hypoxic Conditions ◽  
Transgenic Potato Plants

Transgenic potato plants expressing the nptII-gus marker genes affect survival and development of the Colorado potato beetle

Plant Science ◽  
2002 ◽  
Vol 162 (3) ◽  
pp. 373-380 ◽  
Author(s):  
Sébastien De Turck ◽  
Philippe Giordanengo ◽  
Anas Cherqui ◽  
Corinne Ducrocq-Assaf ◽  
Brigitte S Sangwan-Norreel
Keyword(s):  
Colorado Potato Beetle ◽  
Transgenic Potato ◽  
Marker Genes ◽  
Potato Plants ◽  
Potato Beetle ◽  
Survival And Development ◽  
Transgenic Potato Plants

scholarly journals Molecular studies of Potato mop-top virus (PMTV) in transgenic N. benthamiana and S. tuberosum

2002 ◽  
Vol 38 (SI 1 - 6th Conf EFPP 2002) ◽  
pp. 183-185
Author(s):  
A. Germundsson ◽  
M. Sandgren ◽  
H. Barker ◽  
E.I. Savenkov ◽  
J.P.T. Valkonen
Keyword(s):  
Nicotiana Benthamiana ◽  
Transgenic Potato ◽  
Infested Soil ◽  
Systemic Movement ◽  
Potato Plants ◽  
Cp Gene ◽  
Molecular Studies ◽  
Infested Field ◽  
Transgenic Potato Plants ◽  
Potato Mop Top Virus

Resistance to Potato mop-top virus (PMTV) was studied in Nicotiana benthamiana and potato (Solanum tuberosum cv.<br />Saturna) transformed with the coat protein (CP) gene of PMTV. In N. benthamiana plants mechanically inoculated with<br />PMTV, RNA2 was detected in leaves and roots in several plants in the absence of RNA3. When N. benthamiana was<br />grown in infested soil, viral RNA was detected in roots, but no systemic movement of PMTV to above-ground parts was<br />observed. The incidence of PMTV infections was reduced in the CP-transgenic potato plants grown in an infested field<br />in Sweden. However, in infected tubers, all three virus RNAs were detected.

Thermodynamic and structural properties of tuber starches from transgenic potato plants grown in vitro and in vivo

2015 ◽  
Vol 125 ◽  
pp. 214-223 ◽  
Author(s):  
Luybov A. Wasserman ◽  
Andrey I. Sergeev ◽  
Viktor G. Vasil’ev ◽  
Irina G. Plashchina ◽  
Nina P. Aksenova ◽  
...  
Keyword(s):  
Structural Properties ◽  
Transgenic Potato ◽  
Potato Plants ◽  
Transgenic Potato Plants
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