scholarly journals Phloem-Specific Expression of the Tobacco Mosaic Virus Movement Protein Alters Carbon Metabolism and Partitioning in Transgenic Potato Plants

1997 ◽  
Vol 115 (4) ◽  
pp. 1599-1607 ◽  
Author(s):  
E. Almon ◽  
M. Horowitz ◽  
H. L. Wang ◽  
W. J. Lucas ◽  
E. Zamski ◽  
...  
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Carbon Metabolism ◽  
Movement Protein ◽  
Transgenic Potato ◽  
Virus Movement ◽  
Specific Expression ◽  
Potato Plants ◽  
Transgenic Potato Plants

Comparison of Tobacco Etch Virus and Tobacco Mosaic Virus Enhancers for Expression of Human Calcitonin Gene in Transgenic Potato Plant

2005 ◽  
Vol 277-279 ◽  
pp. 7-11 ◽  
Author(s):  
H. Ofoghi ◽  
N. Moazami ◽  
I. Ivanov
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Transgenic Potato ◽  
Leader Sequence ◽  
Cauliflower Mosaic Virus ◽  
Human Calcitonin ◽  
Agrobacterium Strain ◽  
Potato Plants ◽  
Calcitonin Gene ◽  
Transgenic Potato Plants

The translational enhancement of the 5′-leader sequence of the tobacco mosaic virus (TMV) and tobacco etch viral 5′-leader sequence (TEV) was compared in relation to the expression of the human calcitonin (hCT) gene in transgenic potato plants. The synthetic human calcitonin tetrameric genes were cloned under the control of the 35S Cauliflower mosaic virus (CaMV)promoter linked to the TMV or TEV leader sequences. The resulting constructs were cloned into the binary vector Bin19 and potato minituber discs transformed using an agrobacterium strain. The expression of the hCT gene was studied by mRNA-DNA hybridization, a RT-PCR, ELISA and RIA. The results showed that the TMV omega element increased the yield of the expressed recombinant protein two to three-fold in comparison with the TEV leader sequence.

scholarly journals Challenging the Role of Microtubules in Tobacco Mosaic Virus Movement by Drug Treatments Is Disputable

2006 ◽  
Vol 80 (13) ◽  
pp. 6712-6715 ◽  
Author(s):  
Mark Seemanpillai ◽  
Rabab Elamawi ◽  
Christophe Ritzenthaler ◽  
Manfred Heinlein
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Movement Protein ◽  
Viral Rna ◽  
Virus Movement ◽  
Direct Role ◽  
Microtubule Inhibitors ◽  
Microtubule Polymerization ◽  
Drug Treatments

ABSTRACT The movement protein (MP) of Tobacco mosaic virus interacts with microtubules during infection. Although this interaction is correlated with the function of MP in the cell-to-cell transport of viral RNA, a direct role of microtubules in the movement process was recently challenged by studies involving the treatment of plants with inhibitors of microtubule polymerization. Here, we report evidence suggesting that such treatments may not efficiently disrupt all microtubules. Thus, results obtained from studies using microtubule inhibitors may have to remain open to interpretation with regard to the involvement of microtubules in viral RNA trafficking.

scholarly journals Tobacco mosaic virus Movement Protein Enhances the Spread of RNA Silencing

2008 ◽  
Vol 4 (4) ◽  
pp. e1000038 ◽  
Author(s):  
Hannes Vogler ◽  
Myoung-Ok Kwon ◽  
Vy Dang ◽  
Adrian Sambade ◽  
Monika Fasler ◽  
...  
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Rna Silencing ◽  
Movement Protein ◽  
Virus Movement

Changing Patterns of Localization of the Tobacco Mosaic Virus Movement Protein and Replicase to the Endoplasmic Reticulum and Microtubules during Infection

1998 ◽  
Vol 10 (7) ◽  
pp. 1107 ◽  
Author(s):  
Manfred Heinlein ◽  
Hal S. Padgett ◽  
J. Scott Gens ◽  
Barbara G. Pickard ◽  
Steven J. Casper ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Movement Protein ◽  
Virus Movement ◽  
Changing Patterns

scholarly journals Visualization and characterization of tobacco mosaic virus movement protein binding to single-stranded nucleic acids.

1992 ◽  
Vol 4 (4) ◽  
pp. 397-411 ◽  
Author(s):  
V Citovsky ◽  
M L Wong ◽  
A L Shaw ◽  
B V Prasad ◽  
P Zambryski
Keyword(s):  
Nucleic Acids ◽  
Protein Binding ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Movement Protein ◽  
Virus Movement
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