The Secretion of Active Recombinant Human Gastric Lipase bySaccharomyces cerevisiae

1996 ◽  
Vol 7 (3) ◽  
pp. 229-236 ◽  
Author(s):  
Thomas Crabbe ◽  
A.Neil Weir ◽  
E.Fintan Walton ◽  
Michael E. Brown ◽  
Christopher W. Sutton ◽  
...  
Keyword(s):  
Gastric Lipase

Serum Lipoproteins

1955 ◽  
Vol 1 (2) ◽  
pp. 83-92 ◽  
Author(s):  
Ray K Brown ◽  
Louis S DeLalla ◽  
Dorothy L Kauffman
Keyword(s):  
Fatty Acids ◽  
Lipid Metabolism ◽  
Hydrolytic Enzymes ◽  
Separation And Purification ◽  
Factor Activity ◽  
Serum Lipoproteins ◽  
Diisopropyl Fluorophosphate ◽  
Low Concentrations ◽  
Gastric Lipase ◽  
Enzymatic Nature

Abstract The larger lipoproteins of Sf greater than 10 also contain triglycerides. The action of lipases on these lipoproteins, either heparin-induced clearing factor, pancreatic lipase, or gastric lipase, yields fatty acids, glycerol, and smaller lipoproteins. Crude clearing factor attacks monoglycerides and triglycerides and is inactiveagainst several esters which plasma esterases attack. Albumin, plasma esterases, and probably other components are needed for optimal clearing factor activity. Low concentrations of diisopropyl fluorophosphate (DFP), a substance inhibiting most hydrolytic enzymes, inactivate crude clearing factor. Stoicheometric calculations made from this experiment indicate further the enzymatic nature of clearing factor. Evidence that clearing factor is different from other lipases and from esterases found in plasma is presented. Studies with purified lipoproteins are needed to determine what alterations, if any, occur in smaller lipoproteins. The separation and purification of lipoproteins by chemical and ultracentrifugal means are described. Whole human plasma separated by Cohn's methods 6 and 9 serves as starting material for the preparation of α1 α2 β and Sf 10 and greater lipoproteins. Studies of lipoproteins may give clues to the varied diseases associated with deranged lipid metabolism, such as xanthomatosis and atherosclerosis.

Novel Trifluoromethyl Ketones as Potent Gastric Lipase Inhibitors.

ChemInform ◽  
2003 ◽  
Vol 34 (11) ◽  
Author(s):  
George Kokotos ◽  
Stavroula Kotsovolou ◽  
Robert Verger
Keyword(s):  
Trifluoromethyl Ketones ◽  
Gastric Lipase

Characterization of emulsions and lipolysis of dietary lipids in the human stomach

1994 ◽  
Vol 266 (3) ◽  
pp. G372-G381 ◽  
Author(s):  
M. Armand ◽  
P. Borel ◽  
C. Dubois ◽  
M. Senft ◽  
J. Peyrot ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Lipid Droplets ◽  
Present Finding ◽  
Droplet Diameter ◽  
Human Stomach ◽  
Dietary Lipids ◽  
Gastric Lipase ◽  
A Minor ◽  
First Time

Fasting subjects were intragastrically intubated and received a coarsely emulsified test meal. Gastric aspirates were collected after 1, 2, 3, and 4 h. During digestion in the stomach, unemulsified lipids (> or = 100 microns) represented a minor fraction. A significant amount of the large 70- to 100-microns lipid droplets disappeared, and fine 1- to 10-microns droplets were generated. The median lipid droplet diameter significantly decreased (21.9 vs. 52.9 microns) after 1 h and kept intermediate values for longer periods of time. The emulsion surface area was 100-120 m2/l and was basically provided by 1- to 100-microns droplets. Lipolysis catalyzed by gastric lipase primarily occurred within the first hour of digestion (11.9%). Smaller droplets were enriched in triglyceride lipolytic products. The free fatty acid concentrations were in the range of 5.6-8.2 mM over 1-4 h. The present finding demonstrates for the first time that in the human stomach most dietary lipids are present in the form of emulsified droplets, in the range of 20-40 microns, and that gastric lipolysis can help to increase emulsification in the stomach.

Dog gastric lipase: Stimulation of its secretion in vivo and cytolocalization in mucous pit cells

1992 ◽  
Vol 102 (5) ◽  
pp. 1535-1545 ◽  
Author(s):  
Frédéric Carrière ◽  
Véronique Raphel ◽  
Hervé Moreau ◽  
Alain Bernadac ◽  
Marie-Alix Devaux ◽  
...  
Keyword(s):  
Gastric Lipase ◽  
Pit Cells ◽  
Stimulation Of

Uptake and metabolism of structured triglyceride by Caco-2 cells: reversal of essential fatty acid deficiency

1998 ◽  
Vol 275 (4) ◽  
pp. G652-G659 ◽  
Author(s):  
Johannes H. Spalinger ◽  
Ernest G. Seidman ◽  
Guy Lepage ◽  
Daniel Ménard ◽  
Victor Gavino ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Essential Fatty Acids ◽  
Low Density Lipoprotein ◽  
Structured Lipids ◽  
Density Lipoprotein ◽  
Cellular Level ◽  
Gastric Lipase ◽  
Arachidonic Acids ◽  
Structured Triglyceride ◽  
Uptake And Metabolism

Structured lipids have been proposed as efficient vehicles for the supplementation of essential fatty acids (EFA) to patients with malabsorption. We investigated how a novel structured triglyceride (STG), containing purely octanoic acid in the sn-1/ sn-3 and [14C]linoleic acid in the sn-2 positions, was incorporated into different lipid classes in Caco-2 cells. We also evaluated the contribution of gastric lipase in the uptake and metabolism of [14C]linoleic acid from the STG. We furthermore determined the potential of the STG to correct EFA deficiency induced in Caco-2 cells. The absorption of STG by Caco-2 cells was significantly greater compared with that of triolein. The addition of human gastric lipase significantly enhanced cellular uptake of the labeled substrate, reflecting the stereoselectivity of gastric lipase to hydrolyze medium chain FA. Analysis of the intracellular lipids synthesized revealed a predominance of phospholipids-monoglycerides. Most of the radioactivity in the lipoproteins isolated from Caco-2 cells was recovered in TG-rich lipoproteins (45%) and to a lesser extent in the high-density lipoprotein (36%) and low-density lipoprotein (17%) fractions. The administration of STG to Caco-2 cells rendered EFA deficient produced a marked increase of the cellular level of linoleic and arachidonic acids. This resulted in a lower ratio of 20:3(n-9) to 20:4(n-6), reflecting the correction of EFA deficiency in Caco-2 cells. Our data demonstrate that STG, in the presence of gastric lipase, have beneficial effects on lipid incorporation, lipoprotein production, and EFA status, utilizing Caco-2 cells as a model of EFA deficiency.

Kiwifruit actinidin digests salivary amylase but not gastric lipase

2017 ◽  
Vol 8 (9) ◽  
pp. 3339-3345 ◽  
Author(s):  
Harry Martin ◽  
Sarah B. Cordiner ◽  
Tony K. McGhie
Keyword(s):  
Glycaemic Index ◽  
Salivary Amylase ◽  
Gastric Lipase

Kiwifruit actinidin rapidly digests human salivary amylase: will this lower the glycaemic index when starch and kiwifruit are consumed together?

Ontogeny of human gastric lipase and pepsin activities

1995 ◽  
Vol 108 (6) ◽  
pp. 1650-1656 ◽  
Author(s):  
Daniel Ménard ◽  
Sophie Monfils ◽  
Eric Tremblay
Keyword(s):  
Gastric Lipase
Sign in / Sign up

Export Citation Format

Share Document