Real-Time Multiplex PCR Assays

Methods ◽  
2001 ◽  
Vol 25 (4) ◽  
pp. 430-442 ◽  
Author(s):  
Carl T. Wittwer ◽  
Mark G. Herrmann ◽  
Cameron N. Gundry ◽  
Kojo S.J. Elenitoba-Johnson
Keyword(s):  
Multiplex Pcr ◽  
Real Time ◽  
Pcr Assays

scholarly journals Rapid Multiplex PCR and Real-Time TaqMan PCR Assays for Detection of Salmonella enterica and the Highly Virulent Serovars Choleraesuis and Paratyphi C

2008 ◽  
Vol 46 (12) ◽  
pp. 4018-4022 ◽  
Author(s):  
D. F. Woods ◽  
F. J. Reen ◽  
D. Gilroy ◽  
J. Buckley ◽  
J. G. Frye ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Real Time ◽  
Salmonella Enterica ◽  
Taqman Pcr ◽  
Pcr Assays ◽  
Highly Virulent

scholarly journals Study Comparing Human Papillomavirus (HPV) Real-Time Multiplex PCR and Hybrid Capture II INNO-LiPA v2 HPV Genotyping PCR Assays

2009 ◽  
Vol 47 (7) ◽  
pp. 2106-2113 ◽  
Author(s):  
T. Iftner ◽  
L. Germ ◽  
R. Swoyer ◽  
S. K. Kjaer ◽  
J. G. Breugelmans ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Multiplex Pcr ◽  
Real Time ◽  
Hybrid Capture ◽  
Hpv Genotyping ◽  
Pcr Assays

Rapid and Reliable Detection of Shiga Toxin–Producing Escherichia coli by Real-Time Multiplex PCR

2012 ◽  
Vol 75 (4) ◽  
pp. 643-650 ◽  
Author(s):  
KELLY S. ANKLAM ◽  
KAUSHI S. T. KANANKEGE ◽  
TINA K. GONZALES ◽  
CHARLES W. KASPAR ◽  
DÖRTE DÖPFER
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Multiplex Pcr ◽  
Real Time ◽  
Shiga Toxin ◽  
Virulence Genes ◽  
Health Concern ◽  
E Coli ◽  
Reliable Detection ◽  
Pcr Assays

Escherichia coli O26, O45, O103, O111, O121, O145, and O157 are the predominant Shiga toxin–producing E. coli (STEC) serogroups implicated in outbreaks of human foodborne illness worldwide. The increasing prevalence of these pathogens has important public health implications. Beef products have been considered a main source of foodborne human STEC infections. Robust and sensitive methods for the detection and characterization of these pathogens are needed to determine prevalence and incidence of STEC in beef processing facilities and to improve food safety interventions aimed at eliminating STEC from the food supply. This study was conducted to develop Taqman real-time multiplex PCR assays for the screening and rapid detection of the predominant STEC serogroups associated with human illness. Three serogroup-specific assays targeted the O-antigen gene clusters of E. coli O26 (wzy), O103 (wzx), and O145 (wzx) in assay 1, O45 (wzy), O111 (manC), and O121 (wzx) in assay 2, and O157 (rfbE) in assay 3. The uidA gene also was included in the serogroup-specific assays as an E. coli internal amplification control. A fourth assay was developed to target selected virulence genes for Shiga toxin (stx1 and stx2), intimin (eae), and enterohemolysin (ehxA). The specificity of the serogroup and virulence gene assays was assessed by testing 100 and 62 E. coli strains and non–E. coli control strains, respectively. The assays correctly detected the genes in all strains examined, and no cross-reactions were observed, representing 100% specificity. The detection limits of the assays were 103 or 104 CFU/ml for pure cultures and artificially contaminated fecal samples, and after a 6-h enrichment period, the detection limit of the assays was 100 CFU/ml. These results indicate that the four real-time multiplex PCR assays are robust and effective for the rapid and reliable detection of the seven predominant STEC serogroups of major public health concern and the detection of their virulence genes.

scholarly journals Development of conventional and real-time multiplex PCR assays for the detection of nosocomial pathogens

2011 ◽  
Vol 42 (2) ◽  
pp. 448-458 ◽  
Author(s):  
Deepa Anbazhagan ◽  
Wang Seok Mui ◽  
Marzida Mansor ◽  
Gracie Ong Siok Yan ◽  
Mohd Yasim Yusof ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Real Time ◽  
Nosocomial Pathogens ◽  
Pcr Assays

Real-time multiplex PCR assays for reliable detection of Clostridium perfringens toxin genes in animal isolates

2008 ◽  
Vol 127 (1-2) ◽  
pp. 179-185 ◽  
Author(s):  
S. Albini ◽  
I. Brodard ◽  
A. Jaussi ◽  
N. Wollschlaeger ◽  
J. Frey ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Real Time ◽  
Clostridium Perfringens ◽  
Toxin Genes ◽  
Reliable Detection ◽  
Pcr Assays ◽  
Animal Isolates
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