Cloning and Characterization of the Murine Toll-like Receptor 5 (Tlr5) Gene: Sequence and mRNA Expression Studies in Salmonella-Susceptible MOLF/Ei Mice

Genomics ◽  
2000 ◽  
Vol 64 (3) ◽  
pp. 230-240 ◽  
Author(s):  
Giovanna Sebastiani ◽  
Gary Leveque ◽  
Line Larivière ◽  
Line Laroche ◽  
Emil Skamene ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Gene Sequence ◽  
Toll Like Receptor ◽  
Expression Studies ◽  
Toll Like Receptor 5

scholarly journals Effect of Carotenoids, Oligosaccharides and Anthocyanins on Growth Performance, Immunological Parameters and Intestinal Morphology in Broiler Chickens Challenged with Escherichia coli Lipopolysaccharide

Animals ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 347 ◽  
Author(s):  
Brigitta Csernus ◽  
Sándor Biró ◽  
László Babinszky ◽  
István Komlósi ◽  
András Jávor ◽  
...  
Keyword(s):  
Gene Expression ◽  
Escherichia Coli ◽  
Mrna Expression ◽  
Intestinal Morphology ◽  
Interferon Α ◽  
Toll Like Receptor ◽  
Lps Challenge ◽  
Toll Like Receptor 5 ◽  
Relative Mrna Expression ◽  
Escherichia Coli Lipopolysaccharide

This study was conducted to investigate the effect of carotenoid, oligosaccharide and anthocyanin supplementation in broiler diets under Escherichia coli lipopolysaccharide (LPS) challenge. Ross 308 chickens were fed 5 diets: basal diet (control diet), diet supplemented with β-glucan in 0.05% (positive control) and diets with 0.5% carotenoid-, oligosaccharide- or anthocyanin contents. On the 26th days of age, chickens were challenged intraperitoneally 2 mg LPS per kg of body weight. 12 h after injection, birds were euthanized, then spleen and ileum samples were collected. LPS induced increased relative mRNA expression of splenic (p = 0.0445) and ileal (p = 0.0435) interleukin-1β (IL-1β), which was lower in the spleen in carotenoid (p = 0.0114), oligosaccharide (p = 0.0497) and anthocyanin (p = 0.0303)-treated chickens compared to LPS-injected control birds. Dietary supplementation of carotenoids also decreased relative gene expression of splenic interleukin-6 (IL-6) (p = 0.0325). In the ileum, β-glucan supplementation showed lower relative mRNA expression of toll-like receptor 5 (TLR-5) (p = 0.0387) compared to anthocyanin treatment. Gene expression of both splenic and ileal interferon-α (IFN-α), interferon-γ (IFN-γ), toll-like receptor 4 (TLR-4) and toll-like receptor 5 (TLR-5) were not influenced by dietary supplements. In conclusion, carotenoids, oligosaccharides and anthocyanins could partially mitigate the immune stress caused by LPS challenge. All of the compounds impacted longer villus height (p < 0.0001), villus height:crypt depth ratios were higher after β-glucan (p < 0.0001) and anthocyanin (p = 0.0063) supplementations and thickened mucosa was observed in β-glucan (p < 0.0001), oligosaccharide (p < 0.0001) and anthocyanin (p = 0.048) treatments. All of these findings could represent a more effective absorption of nutrients.

Abstract 5492: Functional characterization of human toll-like receptor 5 (TLR5) genetic variants

2015 ◽  
Author(s):  
Andrzej J. Wierzbicki ◽  
Araba A. Adjei ◽  
Nuttapong Ngamphaiboon ◽  
Thanyanan Reungwetwattana ◽  
Andrei V. Gudkov ◽  
...  
Keyword(s):  
Genetic Variants ◽  
Functional Characterization ◽  
Toll Like Receptor ◽  
Toll Like Receptor 5

Regulation of osteoclastogenesis by toll-like receptor 5

2013 ◽  
Author(s):  
Ali Kassem ◽  
Pernilla Lundberg ◽  
Catharina Lindholm ◽  
Pedro P.C. Souza ◽  
Ulf H. Lerner
Keyword(s):  
Toll Like Receptor ◽  
Toll Like Receptor 5
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