Fatty Acid Cytotoxicity to Bovine Lens Epithelial Cells: Investigations on Cell Viability, ecto-ATPase, Na+, K+-ATPase and Intracellular Sodium Concentrations

2000 ◽  
Vol 71 (4) ◽  
pp. 405-413 ◽  
Author(s):  
Nga Nguyen ◽  
Dagobert Glanz ◽  
Dietmar Glaesser
Keyword(s):  
Fatty Acid ◽  
Epithelial Cells ◽  
Cell Viability ◽  
Intracellular Sodium ◽  
Lens Epithelial Cells ◽  
Bovine Lens

scholarly journals Thioredoxin Binding Protein-2 Regulates Autophagy of Human Lens Epithelial Cells under Oxidative Stress via Inhibition of Akt Phosphorylation

2016 ◽  
Vol 2016 ◽  
pp. 1-17 ◽  
Author(s):  
Jiaojie Zhou ◽  
Ke Yao ◽  
Yidong Zhang ◽  
Guangdi Chen ◽  
Kairan Lai ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Epithelial Cells ◽  
Cell Viability ◽  
Binding Protein ◽  
Negative Regulator ◽  
Human Lens ◽  
Lens Epithelial Cells ◽  
Human Lens Epithelial Cells ◽  
Age Related

Oxidative stress plays an essential role in the development of age-related cataract. Thioredoxin binding protein-2 (TBP-2) is a negative regulator of thioredoxin (Trx), which deteriorates cellular antioxidant system. Our study focused on the autophagy-regulating effect of TBP-2 under oxidative stress in human lens epithelial cells (LECs). Human lens epithelial cells were used for cell culture and treatment. Lentiviral-based transfection system was used for overexpression of TBP-2. Cytotoxicity assay, western blot analysis, GFP/mCherry-fused LC3 plasmid, immunofluorescence, and transmission electronic microscopy were performed. The results showed that autophagic response of LECs with increased LC3-II, p62, and GFP/mCherry-LC3 puncta (P<0.01) was induced by oxidative stress. Overexpression of TBP-2 further strengthens this response and worsens the cell viability (P<0.01). Knockdown of TBP-2 attenuates the autophagic response and cell viability loss induced by oxidative stress. TBP-2 mainly regulates autophagy in the initiation stage, which is mTOR-independent and probably caused by the dephosphorylation of Akt under oxidative stress. These findings suggest a novel role of TBP-2 in human LECs under oxidative stress. Oxidative stress can cause cell injury and autophagy in LECs, and TBP-2 regulates this response. Hence, this study provides evidence regarding the role of TBP-2 in lens and the possible mechanism of cataract development.

Evidence for ATP and ubiquitin dependent degradation of proteins in cultured bovine lens epithelial cells

1991 ◽  
Vol 52 (3) ◽  
pp. 341-347 ◽  
Author(s):  
Jessica Jahngen-Hodge ◽  
Eric Laxman ◽  
Antoinette Zuliani ◽  
Allen Taylor
Keyword(s):  
Epithelial Cells ◽  
Lens Epithelial Cells ◽  
Bovine Lens

Effect of Rabbit Aqueous Humor Obtained after Cataract Surgery on Collagen Gel Contraction Induced by Bovine Lens Epithelial Cells

2000 ◽  
Vol 32 (2-3) ◽  
pp. 94-99 ◽  
Author(s):  
Daijiro Kurosaka ◽  
Katsuhiko Kato ◽  
Takeshi Oshima ◽  
Hiroyo Kurosaka ◽  
Mami Yoshino ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Cataract Surgery ◽  
Aqueous Humor ◽  
Collagen Gel ◽  
Lens Epithelial Cells ◽  
Bovine Lens ◽  
Collagen Gel Contraction

Hydrogen peroxide-induced DNA damage in bovine lens epithelial cells

1990 ◽  
Vol 240 (1) ◽  
pp. 35-45 ◽  
Author(s):  
Norman J. Kleiman ◽  
Ren-Rong Wang ◽  
Abraham Spector
Keyword(s):  
Hydrogen Peroxide ◽  
Dna Damage ◽  
Epithelial Cells ◽  
Lens Epithelial Cells ◽  
Bovine Lens
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