Inducibility of BDNF Gene Promoter I Detected by Calcium-Phosphate-Mediated DNA Transfection Is Confined to Neuronal but Not to Glial Cells

1998 ◽  
Vol 253 (3) ◽  
pp. 818-823 ◽  
Author(s):  
Akiko Tabuchi ◽  
Kuniaki Sano ◽  
Ryuki Nakaoka ◽  
Chikako Nakatani ◽  
Masaaki Tsuda
Keyword(s):  
Calcium Phosphate ◽  
Glial Cells ◽  
Gene Promoter ◽  
Dna Transfection ◽  
Bdnf Gene

scholarly journals Comparison of phenotypic expression with genotypic transformation by using cloned, selectable markers.

1982 ◽  
Vol 2 (5) ◽  
pp. 593-597 ◽  
Author(s):  
P S Linsley ◽  
L Siminovitch
Keyword(s):  
Escherichia Coli ◽  
Herpes Simplex ◽  
Calcium Phosphate ◽  
Cell Lines ◽  
Phenotypic Expression ◽  
Dna Transfection ◽  
Selectable Markers ◽  
Simplex Virus ◽  
Phenotypic Responses

The frequency of phenotypic expression of the herpes simplex virus type 1 tk and Escherichia coli gpt genes was compared with the frequency of genotypic transformation after calcium phosphate-mediated DNA transfection of a number of tk- and hprt- cell lines. In three of the five lines tested, the frequency of phenotypic expression was at most 10-fold higher than that of genotypic transformation as indicated by frequency of HAT resistance. The remaining two lines showed phenotypic responses which were 50- to 100-fold greater than the genotypic responses. The data indicate that the efficiency of DNA-mediated transformation with some cell lines can be limited by events after the uptake and expression of transfected DNA.

Transactivation of proenkephalin gene by HTLV-1 tax1protein in glial cells: Involvement of Fos/Jun complex at an AP-1 element in the proenkephalin gene promoter

1997 ◽  
Vol 3 (1) ◽  
pp. 16-27 ◽  
Author(s):  
William Fu ◽  
Sanjiv R Shah ◽  
Hao Jiang ◽  
Dana C Hilt ◽  
Harish PG Dave ◽  
...  
Keyword(s):  
Glial Cells ◽  
Gene Promoter

REST4-Mediated Modulation of REST/NRSF-Silencing Function during BDNF Gene Promoter Activation

2002 ◽  
Vol 290 (1) ◽  
pp. 415-420 ◽  
Author(s):  
Akiko Tabuchi ◽  
Tomoko Yamada ◽  
Shoko Sasagawa ◽  
Yoshihisa Naruse ◽  
Nozomu Mori ◽  
...  
Keyword(s):  
Gene Promoter ◽  
Bdnf Gene ◽  
Promoter Activation

pDNA and Calcium Phosphate Co-Precipitation on Titanium Alloy Plate

2007 ◽  
Vol 330-332 ◽  
pp. 621-624
Author(s):  
Liang Yang ◽  
Clemens A. van Blitterswijk ◽  
F. Barrere
Keyword(s):  
Titanium Alloy ◽  
Calcium Phosphate ◽  
Calcium Phosphate Coating ◽  
Phosphate Solution ◽  
Dna Transfection ◽  
Alloy Plate ◽  
Promising Strategy ◽  
Co Precipitation ◽  
Titanium Alloy Plate ◽  
Transfection Agent

DNA transfection by biomaterial is a promising strategy to stimulate cells in tissue engineering. Co-precipitation of Calcium Phosphate (CaP) with plasmid DNA (pDNA) is known for several decades as non-viral transfection agent [1]. In this study, we report the co-precipitation of different pDNA into biomimetic calcium phosphate coating onto titanium alloy (Ti) plates in order to evaluate their efficiency of incorporation. And it is possible to tune the rate of coprecipitation by changing the volume of Calcium Phosphate Solution (CPS) and the [pDNA]. The structure of coating was affected by the presence of DNA in CPS solution.

Remote control of activity-dependent BDNF gene promoter-I transcription mediated by REST/NRSF

2009 ◽  
Vol 384 (4) ◽  
pp. 506-511 ◽  
Author(s):  
Daichi Hara ◽  
Mamoru Fukuchi ◽  
Toshihide Miyashita ◽  
Akiko Tabuchi ◽  
Ichiro Takasaki ◽  
...  
Keyword(s):  
Remote Control ◽  
Gene Promoter ◽  
Bdnf Gene ◽  
Activity Dependent
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