scholarly journals Comparison of phenotypic expression with genotypic transformation by using cloned, selectable markers.

1982 ◽  
Vol 2 (5) ◽  
pp. 593-597 ◽  
Author(s):  
P S Linsley ◽  
L Siminovitch
Keyword(s):  
Escherichia Coli ◽  
Herpes Simplex ◽  
Calcium Phosphate ◽  
Cell Lines ◽  
Phenotypic Expression ◽  
Dna Transfection ◽  
Selectable Markers ◽  
Simplex Virus ◽  
Phenotypic Responses

The frequency of phenotypic expression of the herpes simplex virus type 1 tk and Escherichia coli gpt genes was compared with the frequency of genotypic transformation after calcium phosphate-mediated DNA transfection of a number of tk- and hprt- cell lines. In three of the five lines tested, the frequency of phenotypic expression was at most 10-fold higher than that of genotypic transformation as indicated by frequency of HAT resistance. The remaining two lines showed phenotypic responses which were 50- to 100-fold greater than the genotypic responses. The data indicate that the efficiency of DNA-mediated transformation with some cell lines can be limited by events after the uptake and expression of transfected DNA.

Comparison of phenotypic expression with genotypic transformation by using cloned, selectable markers

1982 ◽  
Vol 2 (5) ◽  
pp. 593-597
Author(s):  
P S Linsley ◽  
L Siminovitch
Keyword(s):  
Escherichia Coli ◽  
Herpes Simplex ◽  
Calcium Phosphate ◽  
Cell Lines ◽  
Phenotypic Expression ◽  
Dna Transfection ◽  
Selectable Markers ◽  
Simplex Virus ◽  
Phenotypic Responses

The frequency of phenotypic expression of the herpes simplex virus type 1 tk and Escherichia coli gpt genes was compared with the frequency of genotypic transformation after calcium phosphate-mediated DNA transfection of a number of tk- and hprt- cell lines. In three of the five lines tested, the frequency of phenotypic expression was at most 10-fold higher than that of genotypic transformation as indicated by frequency of HAT resistance. The remaining two lines showed phenotypic responses which were 50- to 100-fold greater than the genotypic responses. The data indicate that the efficiency of DNA-mediated transformation with some cell lines can be limited by events after the uptake and expression of transfected DNA.

scholarly journals Crystal Structure of the B Subunit of Escherichia coli Heat-labile Enterotoxin Carrying Peptides with Anti-herpes Simplex Virus Type 1 Activity

1999 ◽  
Vol 274 (13) ◽  
pp. 8764-8769
Author(s):  
Dubravka Matković-Calogović ◽  
Arianna Loregian ◽  
Maria Rosa D'Acunto ◽  
Roberto Battistutta ◽  
Alessandro Tossi ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Escherichia Coli ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
B Subunit ◽  
Heat Labile ◽  
Simplex Virus

scholarly journals A System for Creating Stable Cell Lines that Express a Gene of Interest from a Bidirectional and Regulatable Herpes Simplex Virus Type 1 Promoter

PLoS ONE ◽  
2015 ◽  
Vol 10 (3) ◽  
pp. e0122253 ◽  
Author(s):  
Christopher B. Chambers ◽  
William P. Halford ◽  
Joshua Geltz ◽  
Olga Villamizar ◽  
Jeffrey Gross ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Cell Lines ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Stable Cell Lines ◽  
Simplex Virus

scholarly journals Cloning of the active thymidine kinase gene of herpes simplex virus type 1 in Escherichia coli K-12.

1979 ◽  
Vol 76 (8) ◽  
pp. 3755-3759 ◽  
Author(s):  
F. Colbere-Garapin ◽  
S. Chousterman ◽  
F. Horodniceanu ◽  
P. Kourilsky ◽  
A. C. Garapin
Keyword(s):  
Escherichia Coli ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Thymidine Kinase ◽  
Herpes Simplex Virus Type ◽  
Thymidine Kinase Gene ◽  
Kinase Gene ◽  
Simplex Virus ◽  
K 12
Sign in / Sign up

Export Citation Format

Share Document