Introduction of a Disulfide Bond into a Cationic Lipid Enhances Transgene Expression of Plasmid DNA

1998 ◽  
Vol 242 (1) ◽  
pp. 141-145 ◽  
Author(s):  
Fuxing Tang ◽  
Jeffrey A Hughes
Keyword(s):  
Disulfide Bond ◽  
Plasmid Dna ◽  
Transgene Expression ◽  
Cationic Lipid

Assembly of Plasmid DNA into Liposomes After Condensation by Cationic Lipid in Anionic Detergent Solution

2005 ◽  
Vol 27 (21) ◽  
pp. 1701-1705 ◽  
Author(s):  
Hong-Wei Zhang ◽  
Ling Zhang ◽  
Xun Sun ◽  
Shu Diao ◽  
Zhi-Rong Zhang
Keyword(s):  
Plasmid Dna ◽  
Cationic Lipid ◽  
Detergent Solution

scholarly journals Plasmid DNA-based Alphavirus Vaccines

Vaccines ◽  
2019 ◽  
Vol 7 (1) ◽  
pp. 29 ◽  
Author(s):  
Kenneth Lundstrom
Keyword(s):  
Tumor Cells ◽  
Plasmid Dna ◽  
Transgene Expression ◽  
Tumor Regression ◽  
Infectious Agents ◽  
Application Range ◽  
Mammalian Cell Lines ◽  
High Level ◽  
Rna Replicon

Alphaviruses have been engineered as vectors for high-level transgene expression. Originally, alphavirus-based vectors were applied as recombinant replication-deficient particles, subjected to expression studies in mammalian and non-mammalian cell lines, primary cell cultures, and in vivo. However, vector engineering has expanded the application range to plasmid DNA-based delivery and expression. Immunization studies with DNA-based alphavirus vectors have demonstrated tumor regression and protection against challenges with infectious agents and tumor cells in animal tumor models. The presence of the RNA replicon genes responsible for extensive RNA replication in the RNA/DNA layered alphavirus vectors provides superior transgene expression in comparison to conventional plasmid DNA-based expression. Immunization with alphavirus DNA vectors revealed that 1000-fold less DNA was required to elicit similar immune responses compared to conventional plasmid DNA. In addition to DNA-based delivery, immunization with recombinant alphavirus particles and RNA replicons has demonstrated efficacy in providing protection against lethal challenges by infectious agents and tumor cells.

Transfection of plasmid DNA by nanocarriers containing a gemini cationic lipid with an aromatic spacer or its monomeric counterpart

2018 ◽  
Vol 161 ◽  
pp. 519-527 ◽  
Author(s):  
María Martínez-Negro ◽  
Ana L. Barrán-Berdón ◽  
Clara Aicart-Ramos ◽  
María L. Moyá ◽  
Conchita Tros de Ilarduya ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Cationic Lipid

scholarly journals Cellular gene transfer mediated by influenza virosomes with encapsulated plasmid DNA

2007 ◽  
Vol 405 (1) ◽  
pp. 41-49 ◽  
Author(s):  
Jørgen de Jonge ◽  
Johanna M. Leenhouts ◽  
Marijke Holtrop ◽  
Pieter Schoen ◽  
Peter Scherrer ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Gradient Centrifugation ◽  
Cationic Lipid ◽  
Sucrose Density Gradient ◽  
Target Cells ◽  
Sucrose Density Gradient Centrifugation ◽  
Viral Membrane ◽  
Viral Envelopes

Reconstituted influenza virosomes (virus membrane envelopes) have been used previously to deliver pDNA (plasmid DNA) bound to their external surface to a variety of target cells. Although high transfection efficiencies can be obtained with these complexes in vitro, the virosome-associated DNA is readily accessible to nucleases and could therefore be prone to rapid degradation under in vivo conditions. In the present study, we show a new method for the production of DNA–virosomes resulting in complete protection of the DNA from nucleases. This method relies on the use of the short-chain phospholipid DCPC (dicaproylphosphatidylcholine) for solubilization of the viral membrane. The solubilized viral membrane components are mixed with pDNA and cationic lipid. Reconstitution of the viral envelopes and simultaneous encapsulation of pDNA is achieved by removal of the DCPC from the mixture through dialysis. Analysis by linear sucrose density-gradient centrifugation revealed that protein, phospholipid and pDNA physically associated to particles, which appeared as vesicles with spike proteins inserted in their membranes when analysed by electron microscopy. The DNA–virosomes retained the membrane fusion properties of the native influenza virus. The virosome-associated pDNA was completely protected from degradation by nucleases, providing evidence for the DNA being highly condensed and encapsulated in the lumen of the virosomes. DNA–virosomes, containing reporter gene constructs, transfected a variety of cell lines, with efficiencies approaching 90%. Transfection was completely dependent on the fusogenic properties of the viral spike protein haemagglutinin. Thus, DNA–virosomes prepared by the new procedure are highly efficient vehicles for DNA delivery, offering the advantage of complete DNA protection, which is especially important for future in vivo applications.

Increased Duration of Transgene Expression in the Lung with Plasmid DNA Vectors Harboring Adenovirus E4 Open Reading Frame 3

1999 ◽  
Vol 10 (11) ◽  
pp. 1833-1843 ◽  
Author(s):  
Nelson S. Yew ◽  
John Marshall ◽  
Malgorzata Przybylska ◽  
Donna M. Wysokenski ◽  
Robin J. Ziegler ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Transgene Expression ◽  
Open Reading Frame ◽  
Reading Frame ◽  
Dna Vectors

Effect of the content of unmethylated CpG dinucleotides in plasmid DNA on the sustainability of transgene expression

2009 ◽  
Vol 11 (5) ◽  
pp. 435-443 ◽  
Author(s):  
Masaru Mitsui ◽  
Makiya Nishikawa ◽  
Lei Zang ◽  
Mitsuru Ando ◽  
Kayoko Hattori ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Transgene Expression ◽  
Cpg Dinucleotides

Effects of Inflammatory Response on In Vivo Transgene Expression by Plasmid DNA in Mice

2008 ◽  
Vol 97 (8) ◽  
pp. 3074-3083 ◽  
Author(s):  
Keiko Kako ◽  
Makiya Nishikawa ◽  
Hiroyuki Yoshida ◽  
Yoshinobu Takakura
Keyword(s):  
Inflammatory Response ◽  
Plasmid Dna ◽  
Transgene Expression
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