Effects of Inflammatory Response on In Vivo Transgene Expression by Plasmid DNA in Mice

2008 ◽  
Vol 97 (8) ◽  
pp. 3074-3083 ◽  
Author(s):  
Keiko Kako ◽  
Makiya Nishikawa ◽  
Hiroyuki Yoshida ◽  
Yoshinobu Takakura
Keyword(s):  
Inflammatory Response ◽  
Plasmid Dna ◽  
Transgene Expression

scholarly journals Plasmid DNA-based Alphavirus Vaccines

Vaccines ◽  
2019 ◽  
Vol 7 (1) ◽  
pp. 29 ◽  
Author(s):  
Kenneth Lundstrom
Keyword(s):  
Tumor Cells ◽  
Plasmid Dna ◽  
Transgene Expression ◽  
Tumor Regression ◽  
Infectious Agents ◽  
Application Range ◽  
Mammalian Cell Lines ◽  
High Level ◽  
Rna Replicon

Alphaviruses have been engineered as vectors for high-level transgene expression. Originally, alphavirus-based vectors were applied as recombinant replication-deficient particles, subjected to expression studies in mammalian and non-mammalian cell lines, primary cell cultures, and in vivo. However, vector engineering has expanded the application range to plasmid DNA-based delivery and expression. Immunization studies with DNA-based alphavirus vectors have demonstrated tumor regression and protection against challenges with infectious agents and tumor cells in animal tumor models. The presence of the RNA replicon genes responsible for extensive RNA replication in the RNA/DNA layered alphavirus vectors provides superior transgene expression in comparison to conventional plasmid DNA-based expression. Immunization with alphavirus DNA vectors revealed that 1000-fold less DNA was required to elicit similar immune responses compared to conventional plasmid DNA. In addition to DNA-based delivery, immunization with recombinant alphavirus particles and RNA replicons has demonstrated efficacy in providing protection against lethal challenges by infectious agents and tumor cells.

scholarly journals Human CD68 promoter GFP transgenic mice allow analysis of monocyte to macrophage differentiation in vivo

Blood ◽  
2014 ◽  
Vol 124 (15) ◽  
pp. e33-e44 ◽  
Author(s):  
Asif J. Iqbal ◽  
Eileen McNeill ◽  
Theodore S. Kapellos ◽  
Daniel Regan-Komito ◽  
Sophie Norman ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Transgenic Mice ◽  
Transgene Expression ◽  
Gfp Expression ◽  
Macrophage Differentiation ◽  
Reporter Mice ◽  
Gfp Reporter ◽  
Key Points ◽  
Macrophage Populations

Key Points CD68-GFP reporter mice show GFP transgene expression in both monocytes and tissue resident macrophage populations. Adoptively transferred CD68-GFP monocytes maintain GFP expression after recruitment in an ongoing inflammatory response.

scholarly journals Transfection of lung cells in vitro and in vivo: effect of antioxidants and intraliposomal bFGF

2003 ◽  
Vol 284 (5) ◽  
pp. L817-L825 ◽  
Author(s):  
Xiaoping Luo ◽  
Rosetta Belcastro ◽  
Judy Cabacungan ◽  
Vicky Hannam ◽  
Anna Negus ◽  
...  
Keyword(s):  
Growth Factor ◽  
Hydroxyl Radical ◽  
Plasmid Dna ◽  
Transgene Expression ◽  
Antioxidant Properties ◽  
Intratracheal Instillation ◽  
Fetal Lung ◽  
Lung Epithelial Cells

We hypothesized that constitutive formation of reactive oxygen species by respiratory cells is a barrier to gene transfer when liposome-DNA complexes are used, by contributing to rapid degradation of plasmid DNA. When plasmid DNA is complexed to liposomes it is protected against H2O2-mediated degradation but not that mediated by the hydroxyl radical. Treatment of distal rat fetal lung epithelial cells (RFL19Ep) with the vitamin E analog Trolox (50 μM) reduced intracellular plasmid degradation. Both Trolox (50 μM) and an iron chelator, phenanthroline (0.1 μM), significantly increased transgene expression in RFL19Ep approximately twofold, consistent with a hydroxyl radical-mediated inhibition of transgene expression. When basic fibroblast growth factor (bFGF; 20 ng/ml), a growth factor with antioxidant properties, was included within liposomes, we observed a significantly greater enhancement of RFL19Ep transgene expression (∼2-fold) over that seen with Trolox or phenanthroline. Inclusion of bFGF within liposomes also significantly enhanced (∼4-fold) transgene expression in mice following intratracheal instillation.

scholarly journals In Vivo Transgene Expression in the Pancreas by the Intraductal Injection of Naked Plasmid DNA

2018 ◽  
Vol 107 (2) ◽  
pp. 647-653 ◽  
Author(s):  
Yuma Yamada ◽  
Mai Tabata ◽  
Jiro Abe ◽  
Masatoshi Nomura ◽  
Hideyoshi Harashima
Keyword(s):  
Plasmid Dna ◽  
Transgene Expression ◽  
Naked Plasmid ◽  
Intraductal Injection ◽  
Naked Plasmid Dna

Inhibitory effects of epimedium herb water extract on the inflammatory response in vitro and in vivo

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
YC Oh ◽  
YH Jeong ◽  
WK Cho ◽  
SJ Lee ◽  
JY Ma
Keyword(s):  
Inflammatory Response ◽  
Water Extract ◽  
Inhibitory Effects
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