Effect of the content of unmethylated CpG dinucleotides in plasmid DNA on the sustainability of transgene expression

2009 ◽  
Vol 11 (5) ◽  
pp. 435-443 ◽  
Author(s):  
Masaru Mitsui ◽  
Makiya Nishikawa ◽  
Lei Zang ◽  
Mitsuru Ando ◽  
Kayoko Hattori ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Transgene Expression ◽  
Cpg Dinucleotides

scholarly journals Long-term Transgene Expression from Plasmid DNA Gene Therapy Vectors Is Negatively Affected by CpG Dinucleotides

2004 ◽  
Vol 10 (2) ◽  
pp. 269-278 ◽  
Author(s):  
Bradley L. Hodges ◽  
Kristin M. Taylor ◽  
Macy F. Joseph ◽  
Sarah A. Bourgeois ◽  
Ronald K. Scheule
Keyword(s):  
Gene Therapy ◽  
Plasmid Dna ◽  
Transgene Expression ◽  
Cpg Dinucleotides ◽  
Gene Therapy Vectors

scholarly journals Plasmid DNA-based Alphavirus Vaccines

Vaccines ◽  
2019 ◽  
Vol 7 (1) ◽  
pp. 29 ◽  
Author(s):  
Kenneth Lundstrom
Keyword(s):  
Tumor Cells ◽  
Plasmid Dna ◽  
Transgene Expression ◽  
Tumor Regression ◽  
Infectious Agents ◽  
Application Range ◽  
Mammalian Cell Lines ◽  
High Level ◽  
Rna Replicon

Alphaviruses have been engineered as vectors for high-level transgene expression. Originally, alphavirus-based vectors were applied as recombinant replication-deficient particles, subjected to expression studies in mammalian and non-mammalian cell lines, primary cell cultures, and in vivo. However, vector engineering has expanded the application range to plasmid DNA-based delivery and expression. Immunization studies with DNA-based alphavirus vectors have demonstrated tumor regression and protection against challenges with infectious agents and tumor cells in animal tumor models. The presence of the RNA replicon genes responsible for extensive RNA replication in the RNA/DNA layered alphavirus vectors provides superior transgene expression in comparison to conventional plasmid DNA-based expression. Immunization with alphavirus DNA vectors revealed that 1000-fold less DNA was required to elicit similar immune responses compared to conventional plasmid DNA. In addition to DNA-based delivery, immunization with recombinant alphavirus particles and RNA replicons has demonstrated efficacy in providing protection against lethal challenges by infectious agents and tumor cells.

Increased Duration of Transgene Expression in the Lung with Plasmid DNA Vectors Harboring Adenovirus E4 Open Reading Frame 3

1999 ◽  
Vol 10 (11) ◽  
pp. 1833-1843 ◽  
Author(s):  
Nelson S. Yew ◽  
John Marshall ◽  
Malgorzata Przybylska ◽  
Donna M. Wysokenski ◽  
Robin J. Ziegler ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Transgene Expression ◽  
Open Reading Frame ◽  
Reading Frame ◽  
Dna Vectors

Effects of Inflammatory Response on In Vivo Transgene Expression by Plasmid DNA in Mice

2008 ◽  
Vol 97 (8) ◽  
pp. 3074-3083 ◽  
Author(s):  
Keiko Kako ◽  
Makiya Nishikawa ◽  
Hiroyuki Yoshida ◽  
Yoshinobu Takakura
Keyword(s):  
Inflammatory Response ◽  
Plasmid Dna ◽  
Transgene Expression
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