Selection of Trypsin Inhibitors in Phage Peptide Library

1996 ◽  
Vol 220 (1) ◽  
pp. 53-56 ◽  
Author(s):  
Rui Fang ◽  
Jie Qi ◽  
Zhi-bin Lu ◽  
Hui Zhou ◽  
Wei Li ◽  
...  
Keyword(s):  
Peptide Library ◽  
Trypsin Inhibitors ◽  
Phage Peptide Library ◽  
Selection Of

scholarly journals Selection of Poly-α 2,8-Sialic Acid Mimotopes from a Random Phage Peptide Library and Analysis of Their Bioactivity

2004 ◽  
Vol 279 (29) ◽  
pp. 30707-30714 ◽  
Author(s):  
Pascal Torregrossa ◽  
Lone Buhl ◽  
Mircea Bancila ◽  
Pascale Durbec ◽  
Claus Schafer ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Peptide Library ◽  
Phage Peptide Library ◽  
Selection Of

scholarly journals A novel method for selection of chymotrypsin inhibitors from a phage peptide library

IUBMB Life ◽  
1998 ◽  
Vol 45 (1) ◽  
pp. 155-161
Author(s):  
Ying Liu ◽  
Bin Li ◽  
Shu Zhang ◽  
Tianyu Li ◽  
Hui Zhou ◽  
...  
Keyword(s):  
Peptide Library ◽  
Novel Method ◽  
Phage Peptide Library ◽  
Chymotrypsin Inhibitors ◽  
Selection Of

scholarly journals Selection of Mimotopes of the Cell Surface Adhesion Molecule Mel-CAM from a Random pVIII-28aa Phage Peptide Library

2002 ◽  
Vol 119 (4) ◽  
pp. 865-869 ◽  
Author(s):  
Christine Hafner ◽  
Ursula Samwald ◽  
Stefan Wagner ◽  
Franco Felici ◽  
Elisabeth Heere-Ress ◽  
...  
Keyword(s):  
Cell Surface ◽  
Adhesion Molecule ◽  
Peptide Library ◽  
Surface Adhesion ◽  
Cell Surface Adhesion Molecule ◽  
Phage Peptide Library ◽  
Selection Of

Mimotope selection of blood group A antigen from a phage display 15-mer peptide library

2013 ◽  
Vol 29 (1-4) ◽  
pp. 39-42 ◽  
Author(s):  
Zhao Ming Tang ◽  
Wei Chao Jiang ◽  
Pan Pan Chang ◽  
Min Fang ◽  
Yu Rong ◽  
...  
Keyword(s):  
Phage Display ◽  
Blood Group ◽  
Peptide Library ◽  
Blood Group A ◽  
Group A ◽  
Selection Of

scholarly journals Novel Method for Selection of Antimicrobial Peptides from a Phage Display Library by Use of Bacterial Magnetic Particles

2008 ◽  
Vol 74 (24) ◽  
pp. 7600-7606 ◽  
Author(s):  
Tsuyoshi Tanaka ◽  
Yoriko Kokuryu ◽  
Tadashi Matsunaga
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Peptides ◽  
Phage Display ◽  
Magnetic Particles ◽  
Peptide Library ◽  
Inner Membrane ◽  
Phage Display Peptide Library ◽  
Wide Range ◽  
Bacterial Magnetic Particles ◽  
Selection Of

ABSTRACT Antimicrobial peptides were isolated from a phage display peptide library using bacterial magnetic particles (BacMPs) as a solid support. The BacMPs obtained from “Magnetospirillum magneticum” strain AMB-1 consist of pure magnetite (50 to 100 nm in size) and are covered with a lipid bilayer membrane derived from the invagination of the inner membrane. BacMPs are easily purified from a culture of magnetotactic bacteria by magnetic separation. Approximately 4 × 1010 PFU of the library phage (complexity, 2.7 × 109) was reacted with BacMPs. The elution of bound phages from BacMPs was performed by disrupting its membrane with phospholipase D treatment. Six candidate peptides, which were highly cationic and could bind onto the BacMP membrane, were obtained. They exhibited antimicrobial activity against Bacillus subtilis but not against Escherichia coli and Saccharomyces cerevisiae. The amino acid substitution of the selected peptide, KPQQHNRPLRHK (peptide 6-7), to enhance the hydrophobicity resulted in obvious antimicrobial activity against all test microorganisms. The present study shows for the first time that a magnetic selection of antimicrobial peptides from the phage display peptide library was successfully achieved by targeting the actual bacterial inner membrane. This BacMP-based method could be a promising approach for a high-throughput screening of antimicrobial peptides targeting a wide range of species.

Identification of novel peptide antagonists for GPIIb/IIIa from a conformationally constrained phage peptide library

1992 ◽  
Vol 14 (4) ◽  
pp. 509-515 ◽  
Author(s):  
Karyn T. O'Neil ◽  
Ronald H. Hoess ◽  
Sharon A. Jackson ◽  
Narayana Swamy Ramachandran ◽  
Shaker A. Mousa ◽  
...  
Keyword(s):  
Peptide Library ◽  
Conformationally Constrained ◽  
Phage Peptide Library
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