scholarly journals 13C Isotopomer Analysis of Glutamate by J-Resolved Heteronuclear Single Quantum Coherence Spectroscopy

2001 ◽  
Vol 289 (2) ◽  
pp. 187-195 ◽  
Author(s):  
Shawn C. Burgess ◽  
Rui A. Carvalho ◽  
Matthew E. Merritt ◽  
John G. Jones ◽  
Craig R. Malloy ◽  
...  
Keyword(s):  
Quantum Coherence ◽  
Heteronuclear Single Quantum Coherence ◽  
Single Quantum ◽  
Isotopomer Analysis ◽  
Coherence Spectroscopy ◽  
13C Isotopomer Analysis

Adhesion/growth-regulatory galectins tested in combination: evidence for formation of hybrids as heterodimers

2018 ◽  
Vol 475 (5) ◽  
pp. 1003-1018 ◽  
Author(s):  
Michelle C. Miller ◽  
Anna-Kristin Ludwig ◽  
Kanin Wichapong ◽  
Herbert Kaltner ◽  
Jürgen Kopitz ◽  
...  
Keyword(s):  
Quantum Coherence ◽  
Cross Linking ◽  
Heteronuclear Single Quantum Coherence ◽  
Single Quantum ◽  
Supportive Evidence ◽  
Cell Binding ◽  
Effector Molecules ◽  
Galectin 3 ◽  
Dynamics Simulations

The delineation of the physiological significance of protein (lectin)–glycan recognition and the structural analysis of individual lectins have directed our attention to studying them in combination. In this report, we tested the hypothesis of hybrid formation by using binary mixtures of homodimeric galectin-1 and -7 as well as a proteolytically truncated version of chimera-type galectin-3. Initial supportive evidence is provided by affinity chromatography using resin-presented galectin-7. Intriguingly, the extent of cell binding by cross-linking of surface counter-receptor increased significantly for monomeric galectin-3 form by the presence of galectin-1 or -7. Pulsed-field gradient NMR (nuclear magnetic resonance) diffusion measurements on these galectin mixtures indicated formation of heterodimers as opposed to larger oligomers. 15N-1H heteronuclear single quantum coherence NMR spectroscopy and molecular dynamics simulations allowed us to delineate how different galectins interact in the heterodimer. The possibility of domain exchange between galectins introduces a new concept for understanding the spectrum of their functionality, particularly when these effector molecules are spatially and temporally co-expressed as found in vivo.

scholarly journals Establishing lignin structure-upgradeability relationships using quantitative 1H–13C heteronuclear single quantum coherence nuclear magnetic resonance (HSQC-NMR) spectroscopy

2019 ◽  
Vol 10 (35) ◽  
pp. 8135-8142 ◽  
Author(s):  
Masoud Talebi Amiri ◽  
Stefania Bertella ◽  
Ydna M. Questell-Santiago ◽  
Jeremy S. Luterbacher
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Nmr Spectroscopy ◽  
Magnetic Resonance ◽  
Quantum Coherence ◽  
Heteronuclear Single Quantum Coherence ◽  
Single Quantum ◽  
Nmr Method ◽  
Nuclear Magnetic ◽  
Lignin Structure

By using a quantitative HSQC-NMR method to measure chemical functionalities within the structure of isolated lignin samples, lignin's upgradability can be very precisely predicted.

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