A Rapid Procedure for Spectral Similarity Matching of Heteronuclear Single Quantum Coherence Spectra

2011 ◽  
Author(s):  
Zhengyi Yang ◽  
Viktor Vegh ◽  
David C. Reutens ◽  
Gregory K. Pierens
Keyword(s):  
Quantum Coherence ◽  
Heteronuclear Single Quantum Coherence ◽  
Single Quantum ◽  
Similarity Matching ◽  
Rapid Procedure ◽  
Spectral Similarity

Adhesion/growth-regulatory galectins tested in combination: evidence for formation of hybrids as heterodimers

2018 ◽  
Vol 475 (5) ◽  
pp. 1003-1018 ◽  
Author(s):  
Michelle C. Miller ◽  
Anna-Kristin Ludwig ◽  
Kanin Wichapong ◽  
Herbert Kaltner ◽  
Jürgen Kopitz ◽  
...  
Keyword(s):  
Quantum Coherence ◽  
Cross Linking ◽  
Heteronuclear Single Quantum Coherence ◽  
Single Quantum ◽  
Supportive Evidence ◽  
Cell Binding ◽  
Effector Molecules ◽  
Galectin 3 ◽  
Dynamics Simulations

The delineation of the physiological significance of protein (lectin)–glycan recognition and the structural analysis of individual lectins have directed our attention to studying them in combination. In this report, we tested the hypothesis of hybrid formation by using binary mixtures of homodimeric galectin-1 and -7 as well as a proteolytically truncated version of chimera-type galectin-3. Initial supportive evidence is provided by affinity chromatography using resin-presented galectin-7. Intriguingly, the extent of cell binding by cross-linking of surface counter-receptor increased significantly for monomeric galectin-3 form by the presence of galectin-1 or -7. Pulsed-field gradient NMR (nuclear magnetic resonance) diffusion measurements on these galectin mixtures indicated formation of heterodimers as opposed to larger oligomers. 15N-1H heteronuclear single quantum coherence NMR spectroscopy and molecular dynamics simulations allowed us to delineate how different galectins interact in the heterodimer. The possibility of domain exchange between galectins introduces a new concept for understanding the spectrum of their functionality, particularly when these effector molecules are spatially and temporally co-expressed as found in vivo.

scholarly journals Establishing lignin structure-upgradeability relationships using quantitative 1H–13C heteronuclear single quantum coherence nuclear magnetic resonance (HSQC-NMR) spectroscopy

2019 ◽  
Vol 10 (35) ◽  
pp. 8135-8142 ◽  
Author(s):  
Masoud Talebi Amiri ◽  
Stefania Bertella ◽  
Ydna M. Questell-Santiago ◽  
Jeremy S. Luterbacher
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Nmr Spectroscopy ◽  
Magnetic Resonance ◽  
Quantum Coherence ◽  
Heteronuclear Single Quantum Coherence ◽  
Single Quantum ◽  
Nmr Method ◽  
Nuclear Magnetic ◽  
Lignin Structure

By using a quantitative HSQC-NMR method to measure chemical functionalities within the structure of isolated lignin samples, lignin's upgradability can be very precisely predicted.

[1H,15N] Heteronuclear Single Quantum Coherence NMR Study of the Mechanism of Aquation of Platinum(IV) Ammine Complexes

2008 ◽  
Vol 47 (17) ◽  
pp. 7673-7680 ◽  
Author(s):  
Murray S. Davies ◽  
Matthew D. Hall ◽  
Susan J. Berners-Price ◽  
Trevor W. Hambley
Keyword(s):  
Quantum Coherence ◽  
Heteronuclear Single Quantum Coherence ◽  
Single Quantum ◽  
Ammine Complexes ◽  
Nmr Study

Multistep degradation method for β-O-4 linkage in lignins: γ-TTSA method. Part 2: reaction of lignin model polymer (DHP)

Holzforschung ◽  
2013 ◽  
Vol 67 (3) ◽  
pp. 249-256 ◽  
Author(s):  
Daisuke Ando ◽  
Fumiaki Nakatsubo ◽  
Toshiyuki Takano ◽  
Hiroshi Nishimura ◽  
Masato Katahira ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Quantum Coherence ◽  
Heteronuclear Single Quantum Coherence ◽  
Single Quantum ◽  
Polymer Model ◽  
Reaction Step ◽  
Ft Ir ◽  
Lignin Model ◽  
Dehydrogenation Polymer ◽  
Ft Ir Spectroscopy

Abstract A selective cleavage method for β-O-4 linkages (the γ-TTSA method) was introduced in previous works, which consists of four reaction steps: (1) γ-tosylation, (2) thioetherification, (3) sulfonylation, and (4) mild alkali degradation. This method was applied to the degradation of a lignin polymer model (dehydrogenation polymer, DHP) consisting of guaiacyl units. Each reaction step was followed by Fourier transform infrared (FT-IR) spectroscopy and heteronuclear single quantum coherence nuclear magnetic resonance (HSQC-NMR) spectroscopy. It was demonstrated that only the β-O-4 linkage was selectively cleaved by the γ-TTSA method, although other linkages, such as β-5 and β-β linkages, were also present in the DHP. Consequently, the γ-TTSA method is expected to be also useful for the degradation of native lignins.

Sign in / Sign up

Export Citation Format

Share Document