Thin-Layer Chromatography and Polyacrylamide Gel Electrophoresis-Based Assays for Sialyltransferases Using Tetramethylrhodamine-Labeled Acceptors

2000 ◽  
Vol 285 (1) ◽  
pp. 92-99 ◽  
Author(s):  
Ulrike Hubl ◽  
George C. Slim ◽  
Olga V. Zubkova
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Layer Chromatography

scholarly journals Studies on alkaline phosphatase isozymes in human serum by using a simple thin layer polyacrylamide gel electrophoresis

1973 ◽  
Vol 17 (2) ◽  
pp. 71-76
Author(s):  
Manabu Masuzawa ◽  
Takenobu Kamata ◽  
Yasuhiko Sakoyama ◽  
Yasuto Chiba ◽  
Zen-ichi Ogita
Keyword(s):  
Alkaline Phosphatase ◽  
Thin Layer ◽  
Human Serum ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel

scholarly journals Electrophoretic studies on human urine amylase isozymes by using a simple thin-layer polyacrylamide gel electrophoresis

1974 ◽  
Vol 19 (2) ◽  
pp. 139-142
Author(s):  
Makoto Otsuki ◽  
Susumu Saeki ◽  
Hosai Yuu ◽  
Tsutomu Kondo ◽  
Shigeaki Baba
Keyword(s):  
Thin Layer ◽  
Human Urine ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Urine Amylase

Phospholipase A2 and Competitive Inhibition of Procoagulant Phospholipids

1975 ◽  
Author(s):  
M. C. Boffa ◽  
G. A. Boffa
Keyword(s):  
Thin Layer ◽  
Phospholipase A2 ◽  
Gel Electrophoresis ◽  
Competitive Inhibition ◽  
Anticoagulant Activity ◽  
Polyacrylamide Gel Electrophoresis ◽  
Phospholipase Activity ◽  
Vipera Berus ◽  
Layer Chromatography ◽  
Gradient Chromatography

A protein with anticoagulant and phospholipase Aa activities was isolated by chromatography, from Vipera berus venom. The phospholipase activity was demonstrated on isolated platelet phospholipids and derivatives were analysed on thin layer chromatography. The strong anticoagulant activity on recalcified platelet poor plasma, is not dependent upon any proteolytic effect. It is due to a competitive inhibition of the phospholipid procoagulant activity. The inhibition constant has been determined.The same molecule appeared to be responsible for both activities as demonstrated by continuous gradient chromatography, polyacrylamide gel electrophoresis and electro-focusing.Inactivation studies permitted to consider that each of both activities was related to a different site on the same molecule.

scholarly journals Separation of serum proteins by using a simple thin layer polyacrylamide gel electrophoresis

1973 ◽  
Vol 17 (2) ◽  
pp. 63-70
Author(s):  
Zen-ichi Ogita ◽  
Yasuhiko Sakoyama ◽  
Yasuto Chiba ◽  
Manabu Masuzawa
Keyword(s):  
Thin Layer ◽  
Gel Electrophoresis ◽  
Serum Proteins ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel
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