scholarly journals Indirect fluorometric detection techniques on thin layer chromatography and effect of ultrasound on gel electrophoresis

1990 ◽  
Author(s):  
Ma. Yinfa
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Gel Electrophoresis ◽  
Fluorometric Detection ◽  
Detection Techniques ◽  
Layer Chromatography

Laser fluorometric detection for thin-layer chromatography

1983 ◽  
Vol 55 (12) ◽  
pp. 1992-1994 ◽  
Author(s):  
Paul B. Huff ◽  
Michael J. Sepaniak
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Fluorometric Detection ◽  
Layer Chromatography

scholarly journals Thin-Layer Chromatography-Densitometry and Liquid Chromatography Analysis of Alkaloids in Leaves of Papaver somniferum Under Stress Conditions

2005 ◽  
Vol 88 (5) ◽  
pp. 1571-1578 ◽  
Author(s):  
Beata Szabó ◽  
Ágnes Lakatos ◽  
Tamás Kõszegi ◽  
György Kátay ◽  
L Botz
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Developmental Stages ◽  
Papaver Somniferum ◽  
Stress Conditions ◽  
Chromatography Method ◽  
Detection Techniques ◽  
Layer Chromatography ◽  
Alkaloid Contents

Abstract The effect of stress conditions on the concentrations of secondary metabolites were examined during various developmental stages of Papaver somniferum plants. P. somniferum plants were grown in laboratory conditions (Budakalász). The experiment consisted of 22 treatments. Significantly different alkaloid contents can be observed under different stress conditions. In general, the alkaloid contents of plants are very low; therefore, a highly sensitive and reliable method has to be developed for analysis. The amount of alkaloids was measured by 2 separation and detection techniques. Accuracy of the thin-layer chromatography method for quantitative analysis is limited. Without purification of samples the background is too noisy. Column liquid chromatography is a sensitive and relatively inexpensive method that allows precise quantitative determination of the alkaloid content.

scholarly journals An Evolving Technology That Integrates Classical Methods with Continuous Technological Developments: Thin-Layer Chromatography Bioautography

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4647
Author(s):  
Meng Wang ◽  
Yirong Zhang ◽  
Ruijie Wang ◽  
Zhibin Wang ◽  
Bingyou Yang ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Ex Situ ◽  
Research Progress ◽  
Antioxidant Compounds ◽  
Detection Techniques ◽  
Tlc Bioautography ◽  
Detection Technology ◽  
Layer Chromatography

Thin-layer chromatography (TLC) bioautography is an evolving technology that integrates the separation and analysis technology of TLC with biological activity detection technology, which has shown a steep rise in popularity over the past few decades. It connects TLC with convenient, economic and intuitive features and bioautography with high levels of sensitivity and specificity. In this study, we discuss the research progress of TLC bioautography and then establish a definite timeline to introduce it. This review summarizes known TLC bioautography types and practical applications for determining antibacterial, antifungal, antitumor and antioxidant compounds and for inhibiting glucosidase, pancreatic lipase, tyrosinase and cholinesterase activity constitutes. Nowadays, especially during the COVID-19 pandemic, it is important to identify original, natural products with anti-COVID potential compounds from Chinese traditional medicine and natural medicinal plants. We also give an account of detection techniques, including in situ and ex situ techniques; even in situ ion sources represent a major reform. Considering the current technical innovations, we propose that the technology will make more progress in TLC plates with higher separation and detection technology with a more portable and extensive scope of application. We believe this technology will be diffusely applied in medicine, biology, agriculture, animal husbandry, garden forestry, environmental management and other fields in the future.

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