scholarly journals Studies on alkaline phosphatase isozymes in human serum by using a simple thin layer polyacrylamide gel electrophoresis

1973 ◽  
Vol 17 (2) ◽  
pp. 71-76
Author(s):  
Manabu Masuzawa ◽  
Takenobu Kamata ◽  
Yasuhiko Sakoyama ◽  
Yasuto Chiba ◽  
Zen-ichi Ogita
Keyword(s):  
Alkaline Phosphatase ◽  
Thin Layer ◽  
Human Serum ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel

scholarly journals Electrophoretic examination of human serum amylase isozymes by using a simple thin layer polyacrylamide gel electrophoresis

1974 ◽  
Vol 18 (2) ◽  
pp. 129-132
Author(s):  
Makoto Otsuki ◽  
Yasuhiko Sakoyama ◽  
Kyung Sook Park ◽  
Zen-ichi Ogita ◽  
Shigeaki Baba ◽  
...  
Keyword(s):  
Thin Layer ◽  
Human Serum ◽  
Gel Electrophoresis ◽  
Serum Amylase ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel

Quantitative Recovery of Alkaline Phosphatase and γ-Glutamyl Transferase Isoenzymes after Polyacrylamide Gel Electrophoresis

1980 ◽  
Vol 17 (1) ◽  
pp. 15-19 ◽  
Author(s):  
TJ Brooks ◽  
HG Sammons
Keyword(s):  
Alkaline Phosphatase ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Glutamyl Transferase ◽  
Quantitative Recovery

Loss of activity of the isoenzymes of γ-glutamyl transferase and alkaline phosphatase has been shown to occur during electrophoresis on polyacrylamide gel. After studying the possible factors concerned in this loss, reasonable recovery from the gel can be obtained only for the isoenzyme staying at the origin. Maximum recovery is 60% for origin γ-glutamyl transferase and 92% for origin alkaline phosphatase.

A comparison of certain isozyme patterns in lobeless and normal embryos of the snail, Ilyanassa obsoleta

Development ◽  
1971 ◽  
Vol 26 (3) ◽  
pp. 339-349
Author(s):  
Sallie B. Freeman
Keyword(s):  
Alkaline Phosphatase ◽  
Enzyme Activity ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Ilyanassa Obsoleta ◽  
Alkaline Phosphatases ◽  
Polar Lobe ◽  
Specific Nature ◽  
Isozyme Patterns

A study was made of the emergence of certain enzymes during the embryogenesis of Ilyanassa. Lobeless and normal embryos were compared in order to determine the effect of polar lobe removal on subsequent molecular developments. Polyacrylamide gel electrophoresis in capillary tubes, a technique requiring only small numbers of embryos, was used to obtain the isozyme patterns of alkaline phosphatases and of esterases. It was found that lobe removal interfered with the emergence of normal isozyme patterns of alkaline phosphatase and esterase during development. Certain bands of enzyme activity were severely reduced or absent while others appeared to be normal. The results provide further evidence that the influence of the polar lobe on development is of a specific nature.

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