Acid Phosphatase Reaction with Peroxyl Radicals: Inactivation Mechanism and Behavior of the Partially Modified Ensemble

2000 ◽  
Vol 379 (2) ◽  
pp. 245-251 ◽  
Author(s):  
A. Aspée ◽  
E.A. Lissi
Keyword(s):  
Acid Phosphatase ◽  
Peroxyl Radicals ◽  
Inactivation Mechanism ◽  
And Behavior ◽  
Acid Phosphatase Reaction

scholarly journals Arrested apoptosis without nuclear fragmentation produced by efferent duct ligation in round spermatids and multinucleated giant cells of rat testis

Reproduction ◽  
2003 ◽  
pp. 879-887 ◽  
Author(s):  
E Anton
Keyword(s):  
Acid Phosphatase ◽  
Nuclear Membrane ◽  
Sertoli Cells ◽  
Giant Cells ◽  
Differential Staining ◽  
Multinucleated Giant Cells ◽  
Nuclear Fragmentation ◽  
Efferent Duct ◽  
Duct Ligation ◽  
Acid Phosphatase Reaction

The apoptotic process evoked by efferent duct ligation in the testes of adult rats was followed for 10 days by differential staining for haematoxylin-eosin, periodic acid-Schiff and a modified trichrome technique in optical microscopy and by ultrastructural localization of acid phosphatase. Round spermatids showed the first effects of efferent duct ligation. At day 3 after ligation, annular clumps of chromatin with typical apoptotic characteristics appeared against the nuclear membrane of these cells. Afterwards, membranous structures and a wide separation between the two layers of the nuclear membrane were observed but nuclear fragmentation did not occur and apoptotic granules were not seen. Cytoplasmic components were also altered, and severely damaged organoids and empty vacuoles lacking acid phosphatase reaction were frequently seen. On day 2 after efferent duct ligation, multinucleated giant cells appeared, which displayed similar characteristics as spermatids and showed no acid phosphatase reaction. Although abnormal spermatids and multinucleated giant cells were surrounded by the cytoplasm of Sertoli cells, neither lysosomal acid phosphatase nor phagocytic activity was detected. It is concluded that efferent duct ligation specifically affects round immature spermatids eliciting a partial nuclear apoptotic response that is not accompanied by autophagic or heterophagic activity and without lysosomal participation in Sertoli cells.

Quantitative X-ray microanalysis of spleen lysosomes after acid phosphatase reaction

1982 ◽  
Vol 75 (4) ◽  
pp. 485-491
Author(s):  
G. M. Roomans ◽  
R. Wróblewski
Keyword(s):  
Acid Phosphatase ◽  
X Ray ◽  
Acid Phosphatase Reaction

scholarly journals HISTOCHEMICAL STUDY OF A FLUORIDE RESISTANT ACID PHOSPHATASE REACTION IN THE MOUSE DUODENUM

1957 ◽  
Vol 5 (2) ◽  
pp. 135-139 ◽  
Author(s):  
ROBERT C. BURT ◽  
BARBARA R. MEREDITH ◽  
ROBERT C. GRAUER
Keyword(s):  
Acid Phosphatase ◽  
Histochemical Study ◽  
Acid Phosphatase Reaction

ACID-PHOSPHATASE REACTION IN ACUTE LYMPHOBLASTIC LEUKÆMIA

The Lancet ◽  
1978 ◽  
Vol 311 (8067) ◽  
pp. 749-751 ◽  
Author(s):  
D. Catovsky ◽  
M.F. Greaves ◽  
C. Pain ◽  
M. Cherchi ◽  
G. Janossy ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Acute Lymphoblastic Leukaemia ◽  
Lymphoblastic Leukaemia ◽  
Acid Phosphatase Reaction

scholarly journals Structural responses of amoebae to the injection of heterologous cytoplasm

1980 ◽  
Vol 45 (1) ◽  
pp. 1-14
Author(s):  
C.J. Flickinger ◽  
G.A. Read ◽  
E.M. Kabana
Keyword(s):  
Acid Phosphatase ◽  
Survival Rate ◽  
Light Microscopy ◽  
Lethal Effect ◽  
Cytoplasmic Vacuoles ◽  
Donor Cells ◽  
Structural Responses ◽  
Cellular Components ◽  
Acid Phosphatase Reaction ◽  
General Appearance

Responses to the introduction of heterologous cytoplasm and the fate of foreign organelles were investigated in amoebae. Heterologous cytoplasm was transferred by microinjection from Pelomyxa carolinensis to Amoeba discoides. In control experiments, homologous cytoplasm was transferred from one A. discoides to another. Recipient cells were observed by light microscopy, and samples were prepared for ultrastructural study at intervals between 15 min nad 3 days after operation. Recipients of heterologous cytoplasm showed two main responses. First, about 40% recipients expelled small amounts of cytoplasm by a blebbing process within 30 min after injection. Second, organelles were segregated and broken down in membrane-bounded cytoplasmic vacuoles between 6 h and 2 days after operation. Acid phosphatase reaction product was observed in these vacuoles along with altered organelles. Use of electron-dense thoria particles to mark donor cells demonstrated the presence of injected cytoplasm in the vacuoles. In contrast, when amoebae were injected with homologous cytoplasm, none was expelled and vacuoles containing degenerating organelles were uncommon. The survival rate and general appearance of recipients of heterologous cytoplasm were much poorer than those of homologous recipients, and most of the former died by I week after operation. It is postulated that amoeba are capable of recognizing heterologous organelles introduced into the cytoplasm and that they respond by expulsion and/or destruction of the foreign cellular components. The previously described lethal effect of heterologous cytoplasm was confirmed.

scholarly journals Acid phosphatase reaction and Alcian Blue-Safranin staining of rat peritoneal mast cells.

1986 ◽  
Vol 19 (6) ◽  
pp. 761-765 ◽  
Author(s):  
SHIRO MIYASHITA ◽  
OSAMU KORETOU ◽  
YASUHIRO IWAI ◽  
NAOKI ARIZONO ◽  
OSAMU TAKEOKA
Keyword(s):  
Acid Phosphatase ◽  
Mast Cells ◽  
Alcian Blue ◽  
Peritoneal Mast Cells ◽  
Rat Peritoneal Mast Cells ◽  
Acid Phosphatase Reaction
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