Identification of a NovelpelDGene Expressed Uniquely in Planta byFusarium solanif. sp.pisi(Nectria haematococca,Mating Type VI) and Characterization of Its Protein Product as an Endo-Pectate Lyase

1996 ◽  
Vol 332 (2) ◽  
pp. 305-312 ◽  
Author(s):  
Wenjin Guo ◽  
Luis González-Candelas ◽  
P.E. Kolattukudy
Keyword(s):  
Mating Type ◽  
Pectate Lyase ◽  
Protein Product ◽  
In Planta ◽  
Nectria Haematococca

scholarly journals Breeding for Highly Fertile Isolates of Nectria haematococca MPVI that are Highly Virulent on Pea and In Planta Selection for Virulent Recombinants

2001 ◽  
Vol 91 (1) ◽  
pp. 92-101 ◽  
Author(s):  
Deanna L. Funnell ◽  
Patty S. Matthews ◽  
Hans D. VanEtten
Keyword(s):  
Mating Type ◽  
Low Fertility ◽  
Female Sterility ◽  
Polymorphism Analysis ◽  
Broad Host Range ◽  
High Fertility ◽  
In Planta ◽  
Nectria Haematococca ◽  
High Virulence ◽  
Highly Virulent

The heterothallic ascomycete Nectria haematococca mating population VI (anamorph Fusarium solani) is a broad host range pathogen. Field isolates of this fungus that are pathogenic on pea tend to be female sterile, of low fertility, and the same mating type (MAT-1), whereas female fertile isolates of either mating type that are highly fertile tend to be nonpathogenic on this plant. To facilitate genetic analysis of traits that may be important in the ability of N. haematococca to parasitize peas, a breeding project was undertaken to produce hermaphroditic isolates of each mating type that are highly fertile and highly virulent on peas. Although the association of high virulence on peas with female sterility and the MAT-1 mating type was not completely broken, isolates with high fertility and high virulence on peas were bred within two generations. Highly virulent progeny were also isolated by an alternative method in which pea plants were inoculated with a mixture of ascospores from a cross between two moderately virulent parents. Whereas all ascospores isolated without selection in planta had lower virulence than the parents, many isolates recovered from diseased tissue were more virulent than the parental isolates. Some of the recovered isolates were shown by restriction fragment length polymorphism analysis to be genetic recombinants of the parents, demonstrating that the pea tissue selected virulent recombinants. All highly virulent isolates tested had the ability to detoxify the pea phytoalexin pisatin, again showing a link between this trait and pathogenicity on the pea.

Cloning and characterization of pl1 encoding an in planta-secreted pectate lyase of Fusarium oxysporum

1999 ◽  
Vol 35 (1) ◽  
pp. 36-40 ◽  
Author(s):  
M. Dolores Huertas-Gonz�lez ◽  
Fe I. Garc�a Maceira ◽  
M. Isabel G. Roncero ◽  
M. Carmen Ruiz-Rold�n ◽  
A. Di Pietro
Keyword(s):  
Fusarium Oxysporum ◽  
Pectate Lyase ◽  
In Planta

scholarly journals Mating Type in Chlamydomonas Is Specified by mid, the Minus-Dominance Gene

Genetics ◽  
1997 ◽  
Vol 146 (3) ◽  
pp. 859-869 ◽  
Author(s):  
Patrick J Ferris ◽  
Ursula W Goodenough
Keyword(s):  
Transcription Factor ◽  
Mating Type ◽  
Codon Bias ◽  
Leucine Zipper ◽  
Laboratory Strain ◽  
Leucine Zipper Motif ◽  
Type Locus ◽  
Diploid Cells ◽  
Necessary And Sufficient

Diploid cells of Chlamydomonas reinhardtii that are heterozygous at the mating-type locus (mt  +/mt  –) differentiate as minus gametes, a phenomenon known as minus dominance. We report the cloning and characterization of a gene that is necessary and sufficient to exert this minus dominance over the plus differentiation program. The gene, called mid, is located in the rearranged (R) domain of the mt  – locus, and has duplicated and transposed to an autosome in a laboratory strain. The imp11 mt  – mutant, which differentiates as a fusion-incompetent plus gamete, carries a point mutation in mid. Like the fus1 gene in the mt  + locus, mid displays low codon bias compared with other nuclear genes. The mid sequence carries a putative leucine zipper motif, suggesting that it functions as a transcription factor to switch on the minus program and switch off the plus program of gametic differentiation. This is the first sex-determination gene to be characterized in a green organism.

scholarly journals Cloning and characterization of four SIR genes of Saccharomyces cerevisiae.

1986 ◽  
Vol 6 (2) ◽  
pp. 688-702 ◽  
Author(s):  
J M Ivy ◽  
A J Klar ◽  
J B Hicks
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Mating Type ◽  
Blot Analysis ◽  
Transcriptional Control ◽  
Genomic Library ◽  
Yeast Saccharomyces Cerevisiae ◽  
Mating Type Genes ◽  
Rna Blots

Mating type in the yeast Saccharomyces cerevisiae is determined by the MAT (a or alpha) locus. HML and HMR, which usually contain copies of alpha and a mating type information, respectively, serve as donors in mating type interconversion and are under negative transcriptional control. Four trans-acting SIR (silent information regulator) loci are required for repression of transcription. A defect in any SIR gene results in expression of both HML and HMR. The four SIR genes were isolated from a genomic library by complementation of sir mutations in vivo. DNA blot analysis suggests that the four SIR genes share no sequence homology. RNA blots indicate that SIR2, SIR3, and SIR4 each encode one transcript and that SIR1 encodes two transcripts. Null mutations, made by replacement of the normal genomic allele with deletion-insertion mutations created in the cloned SIR genes, have a Sir- phenotype and are viable. Using the cloned genes, we showed that SIR3 at a high copy number is able to suppress mutations of SIR4. RNA blot analysis suggests that this suppression is not due to transcriptional regulation of SIR3 by SIR4; nor does any SIR4 gene transcriptionally regulate another SIR gene. Interestingly, a truncated SIR4 gene disrupts regulation of the silent mating type loci. We propose that interaction of at least the SIR3 and SIR4 gene products is involved in regulation of the silent mating type genes.

scholarly journals Distribution and mating type characterization of chickpea blight (Didymella rabiei (Kov.) v. Arx) in Ethiopia

2021 ◽  
pp. 100220
Author(s):  
Gezahegne Getaneh ◽  
Tadele Tefera ◽  
Fikre Lemmessa ◽  
Seid Ahmed ◽  
Jandouwe Villinger
Keyword(s):  
Mating Type
Sign in / Sign up

Export Citation Format

Share Document