The complete sequence of a 9037 bp DNA fragment of the right arm ofSaccharomyces cerevisiae chromosome VII

Yeast ◽  
1995 ◽  
Vol 11 (6) ◽  
pp. 587-591 ◽  
Author(s):  
Javier Arroyo ◽  
Melba García-Gonzalez ◽  
M. Isabel García-Saez ◽  
Miguel Sánchez ◽  
César Nombela
Keyword(s):  
Complete Sequence ◽  
Dna Fragment ◽  
The Right

The complete sequence of a 10·8kb fragment to the right of the chromosome III centromere ofSaccharomyces cerevisiae

Yeast ◽  
1992 ◽  
Vol 8 (1) ◽  
pp. 61-70 ◽  
Author(s):  
Nicolas Biteau ◽  
Christophe Fremaux ◽  
Sylvie Hebrard ◽  
Annie Menara ◽  
Michael Aigle ◽  
...  
Keyword(s):  
Complete Sequence ◽  
Chromosome Iii ◽  
The Right

The complete sequence of a 19,482 bp segment located on the right arm of Chromosome II fromSaccharomyces cerevisiae

Yeast ◽  
1993 ◽  
Vol 9 (2) ◽  
pp. 189-199 ◽  
Author(s):  
Francois Doignon ◽  
Nicolas Biteau ◽  
Marc Crouzet ◽  
Michel Aigle
Keyword(s):  
Complete Sequence ◽  
The Right ◽  
Chromosome Ii

DNA Sequence Analysis of a 23 002 bp DNA Fragment of the Right Arm ofSaccharomyces cerevisiae Chromosome VII

Yeast ◽  
1997 ◽  
Vol 13 (4) ◽  
pp. 357-363 ◽  
Author(s):  
JAVIER ARROYO ◽  
MELBA GARCÍA-GONZALEZ ◽  
M. ISABEL GARCÍA-SAEZ ◽  
MIGUEL SANCHEZ ◽  
CÉSAR NOMBELA
Keyword(s):  
Sequence Analysis ◽  
Dna Sequence ◽  
Dna Sequence Analysis ◽  
Dna Fragment ◽  
The Right

The complete sequence of a 6794 bp segment located on the right arm of chromosome II ofSaccharomyces cerevisiae. Finding of a putative dUTPase in a yeast

Yeast ◽  
1993 ◽  
Vol 9 (10) ◽  
pp. 1131-1137 ◽  
Author(s):  
Francois Doignon ◽  
Nicolas Biteau ◽  
Michel Aigle ◽  
Marc Crouzet
Keyword(s):  
Complete Sequence ◽  
The Right ◽  
Chromosome Ii

II. Yeast sequencing reports. Sequence analysis of a 31 kb DNA fragment from the right arm ofSaccharomyces cerevisiae chromosome II

Yeast ◽  
1994 ◽  
Vol 10 (7) ◽  
pp. 959-964 ◽  
Author(s):  
Quirina J. M. Van Der Aart ◽  
H. Ydesteensma ◽  
Christophe Barthe ◽  
François Doignon ◽  
Michel Aigle ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Dna Fragment ◽  
The Right ◽  
Chromosome Ii

scholarly journals Identification of a Novel Transposon (Tn6072) and a Truncated Staphylococcal Cassette Chromosome mec Element in Methicillin-Resistant Staphylococcus aureus ST239

2010 ◽  
Vol 54 (8) ◽  
pp. 3347-3354 ◽  
Author(s):  
Liang Chen ◽  
José R. Mediavilla ◽  
Davida S. Smyth ◽  
Kalyan D. Chavda ◽  
Ramona Ionescu ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Prototype Strain ◽  
Complete Sequence ◽  
Gene Complex ◽  
Reading Frame ◽  
Clonal Group ◽  
Type Iii ◽  
The Right ◽  
Staphylococcal Cassette Chromosome

ABSTRACT A novel composite transposon (Tn6072) resembling staphylococcal cassette chromosome mercury (SCCHg) was identified in a collection of sequence type (ST) 239 methicillin (meticillin)-resistant Staphylococcus aureus (MRSA) isolates from Romanian hospitals. Tn6072 is homologous to the 5′ region of SCCHg found in staphylococcal cassette chromosome mec (SCCmec) type III prototype strain 85/2082 but lacks the characteristic mer operon. SCCHg has previously been reported to integrate downstream of orfX, at the same chromosomal location as SCCmec. Tn6072, by contrast, is demarcated by two IS431 elements, flanked by 8-bp direct repeats, and inserted upstream of the origin of replication, within an open reading frame homologous to SAR2700 of S. aureus strain MRSA252. Analysis of a geographically and temporally diverse collection of 111 strains from the ST239 clonal group uncovered 11 additional strains harboring Tn6072, demonstrating a lineage-specific insertion pattern. Complete sequence analysis of the SCCmec regions of two representative Romanian strains (BK16704, BK16691) revealed two additional novel structures derived from a type III SCCmec background. BK16704 possesses an SCCmec 3A.1.4 structure, with an IS256 insertion downstream of the right chromosomal junction. In contrast, the SCCmec element of BK16691 is truncated downstream of the mec gene complex, with a 24-kb deletion encompassing the right chromosomal junction and an inverted downstream IS256 element. This structure, tentatively named “ψSCCmec 16691,” confers methicillin resistance but lacks most of the J1/J2 region, including the ccr gene complex. Taken together, these findings provide evidence for the continuing evolution of SCC elements, as well as the ST239 clonal group.

scholarly journals Murine Gammaherpesvirus 68 Contains Two Functional Lytic Origins of Replication

2007 ◽  
Vol 81 (13) ◽  
pp. 7300-7305 ◽  
Author(s):  
Heiko Adler ◽  
Beatrix Steer ◽  
Klaus Freimüller ◽  
Jürgen Haas
Keyword(s):  
Artificial Chromosome ◽  
Plasmid Replication ◽  
Origin Of Replication ◽  
Replication Assay ◽  
Murine Gammaherpesvirus ◽  
Origins Of Replication ◽  
Dna Fragment ◽  
Gammaherpesvirus 68 ◽  
The Right ◽  
Murine Gammaherpesvirus 68

ABSTRACT A 1.25-kbp DNA fragment from the right side of the genome containing the lytic origin of replication (oriLyt) of murine gammaherpesvirus 68 (MHV-68) has been identified by a plasmid replication assay. Here we show that a mutant MHV-68 with a deletion of an essential part of this oriLyt, generated by using an MHV-68 bacterial artificial chromosome, was only slightly attenuated and still able to replicate but that a mutant containing an additional deletion on the left side of the genome was replication deficient. The newly identified region was sufficient to support plasmid replication, thus providing evidence for a second oriLyt.

DNA Fragment Assembly Using Multi-Objective Genetic Algorithms

2014 ◽  
Vol 5 (3) ◽  
pp. 84-108 ◽  
Author(s):  
Manisha Rathee ◽  
T. V. Vijay Kumar
Keyword(s):  
Optimization Problem ◽  
Nsga Ii ◽  
Fragment Assembly ◽  
Assembly Algorithm ◽  
Multi Objective ◽  
Multi Objective Genetic Algorithm ◽  
Dna Fragment ◽  
The Right ◽  
Dna Fragment Assembly ◽  
Single Objective

DNA Fragment Assembly Problem (FAP) is concerned with the reconstruction of the target DNA, using the several hundreds (or thousands) of sequenced fragments, by identifying the right order and orientation of each fragment in the layout. Several algorithms have been proposed for solving FAP. Most of these have solely dwelt on the single objective of maximizing the sum of the overlaps between adjacent fragments in order to optimize the fragment layout. This paper aims to formulate this FAP as a bi-objective optimization problem, with the two objectives being the maximization of the overlap between the adjacent fragments and the minimization of the overlap between the distant fragments. Moreover, since there is greater desirability for having lesser number of contigs, FAP becomes a tri-objective optimization problem where the minimization of the number of contigs becomes the additional objective. These problems were solved using the multi-objective genetic algorithm NSGA-II. The experimental results show that the NSGA-II-based Bi-Objective Fragment Assembly Algorithm (BOFAA) and the Tri-Objective Fragment Assembly Algorithm (TOFAA) are able to produce better quality layouts than those generated by the GA-based Single Objective Fragment Assembly Algorithm (SOFAA). Further, the layouts produced by TOFAA are also comparatively better than those produced using BOFAA.

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