Sequence and functional analysis of a 7·2 kb DNA fragment containing four open reading frames located betweenRPB5 andCDC28 on the right arm of chromosome II

Yeast ◽  
1995 ◽  
Vol 11 (9) ◽  
pp. 865-871 ◽  
Author(s):  
Matthias Rose ◽  
Peter Kiesau ◽  
Markus Proft ◽  
Karl-Dieter Entian
Keyword(s):  
Functional Analysis ◽  
Open Reading Frames ◽  
Dna Fragment ◽  
The Right ◽  
Chromosome Ii ◽  
Reading Frames

Sequencing and functional analysis of a 32 560 bp segment on the left arm of yeast chromosome II. Identification of 26 open reading frames, including theKIP1 andSEC17 genes

Yeast ◽  
1993 ◽  
Vol 9 (12) ◽  
pp. 1355-1371 ◽  
Author(s):  
Bart Scherens ◽  
Mohamed El Bakkoury ◽  
Fabienne Vierendeels ◽  
Evelyne Dubois ◽  
Francine Messenguy
Keyword(s):  
Functional Analysis ◽  
Open Reading Frames ◽  
Yeast Chromosome ◽  
Chromosome Ii ◽  
Reading Frames

Functional analysis of three adjacent open reading frames from the right arm of yeast chromosome XVI

Yeast ◽  
1998 ◽  
Vol 14 (11) ◽  
pp. 1027-1039 ◽  
Author(s):  
Beata Waśkiewicz-Staniorowska ◽  
Jacek Skała ◽  
Michał Jasiński ◽  
Marcelle Grenson ◽  
André Goffeau ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Open Reading Frames ◽  
Yeast Chromosome ◽  
The Right ◽  
Reading Frames

II. Yeast Sequencing Reports. The sequence of 29·7 kb from the right arm of chromosome II reveals 13 complete open reading frames, of which ten correspond to new genes

Yeast ◽  
1994 ◽  
Vol 10 (S1994A) ◽  
pp. S1-S11 ◽  
Author(s):  
A.-M. Bécam ◽  
C. Cullin ◽  
E. Grzybowska ◽  
F. Lacroute ◽  
F. Nasr ◽  
...  
Keyword(s):  
Open Reading Frames ◽  
New Genes ◽  
The Right ◽  
Chromosome Ii ◽  
Reading Frames

scholarly journals Genetic Investigation of the Catabolic Pathway for Degradation of Abietane Diterpenoids by Pseudomonas abietaniphilaBKME-9

2000 ◽  
Vol 182 (13) ◽  
pp. 3784-3793 ◽  
Author(s):  
Vincent J. J. Martin ◽  
William W. Mohn
Keyword(s):  
Gene Cluster ◽  
Sequence Similarity ◽  
Open Reading Frames ◽  
Ring Cleavage ◽  
Catabolic Pathway ◽  
Transcriptional Fusion ◽  
Abietane Diterpenoids ◽  
Genes Encoding ◽  
Dna Fragment ◽  
Reading Frames

ABSTRACT We have cloned and sequenced the dit gene cluster encoding enzymes of the catabolic pathway for abietane diterpenoid degradation by Pseudomonas abietaniphila BKME-9. Thedit gene cluster is located on a 16.7-kb DNA fragment containing 13 complete open reading frames (ORFs) and 1 partial ORF. The genes ditA1A2A3 encode the α and β subunits and the ferredoxin of the dioxygenase which hydroxylates 7-oxodehydroabietic acid to 7-oxo-11,12-dihydroxy-8,13-abietadien acid. The dioxygenase mutant strain BKME-941 (ditA1::Tn5) did not grow on nonaromatic abietanes, and transformed palustric and abietic acids to 7-oxodehydroabietic acid in cell suspension assays. Thus, nonaromatic abietanes are aromatized prior to further degradation. Catechol 2,3-dioxygenase activity of xylEtranscriptional fusion strains showed induction of ditA1and ditA3 by abietic, dehydroabietic, and 7-oxodehydroabietic acids, which support the growth of strain BKME-9, as well as by isopimaric and 12,14-dichlorodehydroabietic acids, which are diterpenoids that do not support the growth of strain BKME-9. In addition to the aromatic-ring-hydroxylating dioxygenase genes, thedit cluster includes ditC, encoding an extradiol ring cleavage dioxygenase, and ditR, encoding an IclR-type transcriptional regulator. Although ditR is not strictly required for the growth of strain BKME-9 on abietanes, aditR::Kmr mutation in aditA3::xylE reporter strain demonstrated that it encodes an inducer-dependent transcriptional activator of ditA3. An ORF with sequence similarity to genes encoding permeases (ditE) is linked with genes involved in abietane degradation.

scholarly journals First Staphylococcal Cassette ChromosomemecContaining amecB-Carrying Gene Complex Independent of Transposon Tn6045in a Macrococcus caseolyticus Isolate from a Canine Infection

2015 ◽  
Vol 59 (8) ◽  
pp. 4577-4583 ◽  
Author(s):  
Elena Gómez-Sanz ◽  
Sybille Schwendener ◽  
Andreas Thomann ◽  
Stefanie Gobeli Brawand ◽  
Vincent Perreten
Keyword(s):  
Integration Site ◽  
Direct Repeat ◽  
Open Reading Frames ◽  
Gene Complex ◽  
Direct Repeats ◽  
Content Type ◽  
Canine Infection ◽  
The Right ◽  
Lactamase Gene ◽  
Reading Frames

ABSTRACTA methicillin-resistantmecB-positiveMacrococcus caseolyticus(strain KM45013) was isolated from the nares of a dog with rhinitis. It contained a novel 39-kb transposon-defective completemecB-carrying staphylococcal cassette chromosomemecelement (SCCmecKM45013). SCCmecKM45013contained 49 coding sequences (CDSs), was integrated at the 3′ end of the chromosomalorfXgene, and was delimited at both ends by imperfect direct repeats functioning as integration site sequences (ISSs). SCCmecKM45013presented two discontinuous regions of homology (SCCmeccoverage of 35%) to the chromosomal and transposon Tn6045-associated SCCmec-like element ofM. caseolyticusJCSC7096: (i) themecgene complex (98.8% identity) and (ii) theccr-carrying segment (91.8% identity). Themecgene complex, located at the right junction of the cassette, also carried the β-lactamase geneblaZm(mecRm-mecIm-mecB-blaZm). SCCmecKM45013contained two cassette chromosome recombinase genes,ccrAm2andccrBm2, which shared 94.3% and 96.6% DNA identity with those of the SCCmec-like element of JCSC7096 but shared less than 52% DNA identity with the staphylococcalccrABandccrCgenes. Three distinct extrachromosomal circularized elements (the entire SCCmecKM45013, ΨSCCmecKM45013lacking theccrgenes, and SCCKM45013lackingmecB) flanked by one ISS copy, as well as the chromosomal regions remaining after excision, were detected. An unconventional circularized structure carrying themecBgene complex was associated with two extensive direct repeat regions, which enclosed two open reading frames (ORFs) (ORF46 and ORF51) flanking the chromosomalmecB-carrying gene complex. This study revealedM. caseolyticusas a potential disease-associated bacterium in dogs and also unveiled an SCCmecelement carryingmecBnot associated with Tn6045in the genusMacrococcus.

A 9359 bp fragment from the right arm ofSaccharomyces cerevisiae chromosome VII includes theFOL2 andYTA7 genes and three unknown open reading frames

Yeast ◽  
1998 ◽  
Vol 14 (6) ◽  
pp. 587-591 ◽  
Author(s):  
M. L. Agostoni Carbone ◽  
G. Lucchini ◽  
P. Melchioretto ◽  
V. Nardese ◽  
M. Vanoni ◽  
...  
Keyword(s):  
Open Reading Frames ◽  
The Right ◽  
Reading Frames
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