The complete sequence of a 10·8kb fragment to the right of the chromosome III centromere ofSaccharomyces cerevisiae

Yeast ◽  
1992 ◽  
Vol 8 (1) ◽  
pp. 61-70 ◽  
Author(s):  
Nicolas Biteau ◽  
Christophe Fremaux ◽  
Sylvie Hebrard ◽  
Annie Menara ◽  
Michael Aigle ◽  
...  
Keyword(s):  
Complete Sequence ◽  
Chromosome Iii ◽  
The Right

scholarly journals MULTIPLE GENE SITES FOR 5S AND 18 + 28S RNA ON CHROMOSOMES OF GLYPTOTENDIPES BARBIPES (STAEGER)

1974 ◽  
Vol 62 (1) ◽  
pp. 132-144 ◽  
Author(s):  
Wu-Nan Wen ◽  
Pedro E. León ◽  
Donald R. Hague
Keyword(s):  
Drosophila Melanogaster ◽  
Salivary Gland ◽  
Nucleolar Organizer ◽  
Balbiani Ring ◽  
5S Rna ◽  
Multiple Gene ◽  
Main Site ◽  
Chromosome Iii ◽  
The Right

Ribosomal RNAs (28 + 18S and 5S) and 4S RNA extracted from the chironomid Glyptotendipes barbipes were iodinated in vitro with 125I and hybridized to the salivary gland chromosomes of G. barbipes and Drosophila melanogaster. Iodinated 18 + 28 S RNA labeled three puffed sites with associated nucleoli on chromosomes IR, IIL, and IIIL of G. barbipes and the nucleolar organizer of Drosophila. Labeled 5S RNA hybridized to three sites on chromosome IIIR, two sites on chromosome IIR and one site in a Balbiani ring on chromosome IV of Glyptotendipes. Most of the label produced by this RNA was localized seven bands away from the centromere on the right arm of chromosome III, and we consider this to be the main site complementary to 5S RNA in the chironomid. This same RNA preparation specifically labeled the 56 EF region of chromosome IIR of Drosophila which has been shown previously to be the only site labeled when hybridized with homologous 5S RNA. Hybridization of G. barbipes chromosomes with iodinated 4S RNA produced no clearly localized labeled sites over the exposure periods studied.

The complete sequence of a 9037 bp DNA fragment of the right arm ofSaccharomyces cerevisiae chromosome VII

Yeast ◽  
1995 ◽  
Vol 11 (6) ◽  
pp. 587-591 ◽  
Author(s):  
Javier Arroyo ◽  
Melba García-Gonzalez ◽  
M. Isabel García-Saez ◽  
Miguel Sánchez ◽  
César Nombela
Keyword(s):  
Complete Sequence ◽  
Dna Fragment ◽  
The Right

scholarly journals Isolation and genetic analysis of Saccharomyces cerevisiae mutants supersensitive to G1 arrest by a factor and alpha factor pheromones.

1982 ◽  
Vol 2 (1) ◽  
pp. 11-20 ◽  
Author(s):  
R K Chan ◽  
C A Otte
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Mating Type ◽  
Solid Medium ◽  
G1 Arrest ◽  
Alpha Cells ◽  
Opposite Mating Type ◽  
Alpha Factor ◽  
Complementation Groups ◽  
Chromosome Iii ◽  
The Right

Eight independently isolated mutants which are supersensitive (Sst-) to the G1 arrest induced by the tridecapeptide pheromone alpha factor were identified by screening mutagenized Saccharomyces cerevisiae MATa cells on solid medium for increased growth inhibition by alpha factor. These mutants carried lesions in two complementation groups, sst1 and sst2. Mutations at the sst1 locus were mating type specific: MATa sst1 cells were supersensitive to alpha factor, but MAT alpha sst1 cells were not supersensitive to a factor. In contrast, mutations at the sst2 locus conferred supersensitivity to the pheromones of the opposite mating type on both MATa and MAT alpha cells. Even in the absence of added alpha pheromone, about 10% of the cells in exponentially growing cultures of MATa strains carrying any of three different alleles of sst2 (including the ochre mutation sst2-4) had the aberrant morphology ("shmoo" shape) that normally develops only after MATa cells are exposed to alpha factor. This "self-shmooing" phenotype was genetically linked to the sst2 mutations, although the leakiest allele isolated (sst2-3) did not display this characteristic. Normal MATa/MAT alpha diploids do not respond to pheromones; diploids homozygous for an sst2 mutation (MATa/MAT alpha sst2-1/sst2-1) were still insensitive to alpha factor. The sst1 gene was mapped to within 6.9 centimorgans of his6 on chromosome IX. The sst2 gene was unlinked to sst1, was not centromere linked, and was shown to be neither linked to nor centromere distal to MAT on the right arm of chromosome III.

The complete sequence of a 19,482 bp segment located on the right arm of Chromosome II fromSaccharomyces cerevisiae

Yeast ◽  
1993 ◽  
Vol 9 (2) ◽  
pp. 189-199 ◽  
Author(s):  
Francois Doignon ◽  
Nicolas Biteau ◽  
Marc Crouzet ◽  
Michel Aigle
Keyword(s):  
Complete Sequence ◽  
The Right ◽  
Chromosome Ii

The complete sequence of a 6794 bp segment located on the right arm of chromosome II ofSaccharomyces cerevisiae. Finding of a putative dUTPase in a yeast

Yeast ◽  
1993 ◽  
Vol 9 (10) ◽  
pp. 1131-1137 ◽  
Author(s):  
Francois Doignon ◽  
Nicolas Biteau ◽  
Michel Aigle ◽  
Marc Crouzet
Keyword(s):  
Complete Sequence ◽  
The Right ◽  
Chromosome Ii

scholarly journals RESOLUTION OF DICENTRIC CHROMOSOMES BY TY-MEDIATED RECOMBINATION IN YEAST

Genetics ◽  
1985 ◽  
Vol 110 (3) ◽  
pp. 397-419
Author(s):  
Richard T Surosky ◽  
Bik-Kwoon Tye
Keyword(s):  
Centromeric Region ◽  
Ring Chromosome ◽  
Normal Chromosome ◽  
Meiotic Segregation ◽  
Linear Chromosome ◽  
Ty Elements ◽  
Dicentric Chromosomes ◽  
Chromosome Iii ◽  
The Right ◽  
Diploid Cells

ABSTRACT We have integrated a plasmid containing a yeast centromere, CEN5, into the HIS4 region of chromosome III by transformation. Of the three transformant colonies examined, none contained a dicentric chromosome, but all contained a rearranged chromosome III. In one transformant, rearrangement occurred by homologous recombination between two Ty elements; one on the left arm and the other on the right arm of chromosome III. This event produced a ring chromosome (ring chromosome III) of about 60 kb consisting of CEN3 and all other sequences between the two Ty elements. In addition, a linear chromosome (chromosome IIIA) consisting of sequences distal to the two Ty elements including CEN5, but lacking 60 kb of sequences from the centromeric region, was produced. Two other transformants also contain a similarly altered linear chromosome III as well as an apparently normal copy of chromosome III. These results suggest that dicentric chromosomes cannot be maintained in yeast and that dicentric structures must be resolved for the cell to survive.—The meiotic segregation properties of ring chromosome III and linear chromosome IIIA were examined in diploid cells which also contained a normal chromosome III. Chromosome IIIA and normal chromosome III disjoined normally, indicating that homology or parallel location of the centromeric regions of these chromosomes are not essential for proper meiotic segregation. In contrast, the 60-kb ring chromosome III, which is homologous to the centromeric region of the normal chromosome III, did not appear to pair with fidelity with chromosome III.

Rules of Donor Preference in Saccharomyces Mating-Type Gene Switching Revealed by a Competition Assay Involving Two Types of Recombination

Genetics ◽  
1997 ◽  
Vol 147 (2) ◽  
pp. 399-407 ◽  
Author(s):  
Xiaohua Wu ◽  
Cherry Wu ◽  
James E Haber
Keyword(s):  
Mating Type ◽  
Strand Break ◽  
Competition Assay ◽  
A Cells ◽  
Single Strand Annealing ◽  
Type Gene ◽  
Chromosome Iii ◽  
Strand Annealing ◽  
The Right ◽  
Donor Preference

Mating type (MAT) switching in Saccharomyces cerevisiae is initiated by a double-strand break (DSB) created at MAT by HO endonuclease. MAT  a cells activate the entire left arm of chromosome III; thus MAT  a preferentially recombines with the silent donor HML. In contrast, MATα cells inactivate the left arm, including HML, and thus preferentially recombine with HMR, 100 kb to the right of MAT. We present a novel competition assay, in which the DSB at MAT can be repaired either by MAT switching or by single-strand annealing (SSA) between two URA3 genes flanking MAT. With preferred donors, MAT  a or MATα switching occurs 65–70% of the time in competition with SSA. When HML is deleted, 40% of MAT  a cells recombine with the “wrong” donor HMR; however, when HMR is deleted, only 18% of MATα cells recombine with HML. In interchromosomal switching, with donors on chromosome III and MAT on chromosome V, MAT  a retains its strong preference for HML and switching is efficient, when the chromosome III recombination enhancer is present. However, MATα donor preference is lost and interchromosomal switching is very inefficient. These experiments demonstrate the utility of using competition between two outcomes to measure the relative efficiency of recombination.

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