Generation of glycerophospholipid molecular species in the yeastSaccharomyces cerevisiae. Fatty acid pattern of phospholipid classes and selective acyl turnover atsn-1 andsn-2 positions

Yeast ◽  
1994 ◽  
Vol 10 (11) ◽  
pp. 1429-1437 ◽  
Author(s):  
S. Wagner ◽  
F. Paltauf
Keyword(s):  
Fatty Acid ◽  
Molecular Species ◽  
Fatty Acid Pattern ◽  
Phospholipid Classes

Phosphatidylethanolamine molecular species of fatty acid auxotroph of Escherichia coli grown with elaidate

1975 ◽  
Vol 409 (2) ◽  
pp. 212-217 ◽  
Author(s):  
Nishihara Masateru ◽  
Kimura Kazuko ◽  
Izui Katsura ◽  
Ishinaga Masataka ◽  
Kato Michie ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fatty Acid ◽  
Molecular Species ◽  
Fatty Acid Auxotroph

scholarly journals Evidence for the regulation of guinea-pig heart microsomal phosphatidylcholine-hydrolysing phospholipase A1 by guanosine 5′-[γ-thio]triphosphate

1992 ◽  
Vol 288 (3) ◽  
pp. 965-968 ◽  
Author(s):  
K Badiani ◽  
X Lu ◽  
G Arthur
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Guinea Pig ◽  
Molecular Species ◽  
Inhibitory Effect ◽  
Phospholipase A1 ◽  
Guinea Pig Heart ◽  
Substrate Specificities ◽  
Rate Of Hydrolysis ◽  
Hydrolysis Of

We have recently characterized lysophospholipase A2 activities in guinea-pig heart microsomes and postulated that these enzymes act sequentially with phospholipases A1 to release fatty acids selectively from phosphatidylcholine (PC) and phosphatidylethanolamine, thus providing an alternative route to the phospholipase A2 mode of release. In a further investigation of the postulated pathway, we have characterized the PC-hydrolysing phospholipase A1 in guinea-pig heart microsomes. Our results show that the enzyme may have a preference for substrates with C16:0 over C18:0 at the sn-1 position. In addition, although the enzyme cleaves the sn-1 fatty acid, the rate of hydrolysis of PC substrates with C16:0 at the sn-1 position was influenced by the nature of the fatty acid at the sn-2 position. The order of decreasing preference was C18:2 > C20:4 = C18:1 > C16:0. The hydrolyses of the molecular species were differentially affected by heating at 60 degrees C. An investigation into the effect of nucleotides on the activity of the enzyme showed that guanosine 5′-[gamma-thio]triphosphate (GTP[S]) inhibited the hydrolysis of PC by phospholipase A1 activity, whereas GTP, guanosine 5′-[beta-thio]diphosphate (GDP[S]), GDP, ATP and adenosine 5′-[gamma-thio]triphosphate (ATP[S]) did not affect the activity. The inhibitory effect of GTP[S] on phospholipase A1 activity was blocked by preincubation with GDP[S]. A differential effect of GTP[S] on the hydrolysis of different molecular species was also observed. Taken together, the results of this study suggest the presence of more than one phospholipase A1 in the microsomes with different substrate specificities, which act sequentially with lysophospholipase A2 to release linoleic or arachidonic acid selectively from PC under resting conditions. Upon stimulation and activation of the G-protein, the release of fatty acids would be inhibited.

scholarly journals Phospholipid classes and fatty acid composition of ewe’s and goat’s milk

2013 ◽  
Vol 64 (3) ◽  
pp. 304-310 ◽  
Author(s):  
P. Hueso ◽  
L. Zancada ◽  
F. Pérez-Díez ◽  
F. Sánchez-Juanes ◽  
J. M. Alonso ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Goat’S Milk ◽  
Phospholipid Classes ◽  
Goat's Milk

Crude palm oil from interspecific hybrid Elaeis oleifera×Elaeis guineensis: Fatty acid regiodistribution and molecular species of glycerides

2013 ◽  
Vol 141 (1) ◽  
pp. 245-252 ◽  
Author(s):  
Massimo Mozzon ◽  
Deborah Pacetti ◽  
Paolo Lucci ◽  
Michele Balzano ◽  
Natale Giuseppe Frega
Keyword(s):  
Fatty Acid ◽  
Palm Oil ◽  
Molecular Species ◽  
Interspecific Hybrid ◽  
Elaeis Guineensis ◽  
Crude Palm Oil ◽  
Elaeis Oleifera

scholarly journals Molecular Species of the Alcohol Biomarker Phosphatidylethanol in Human Blood Measured by LC-MS

2009 ◽  
Vol 55 (7) ◽  
pp. 1395-1405 ◽  
Author(s):  
Anders Helander ◽  
Yufang Zheng
Keyword(s):  
Fatty Acid ◽  
Human Blood ◽  
Molecular Species ◽  
Quantitative Measurement ◽  
Selected Ion Monitoring ◽  
Blood Samples ◽  
Qualitative And Quantitative ◽  
Ms Analysis ◽  
Alcohol Biomarker ◽  
Product Ions

Abstract Background: The alcohol biomarker phosphatidylethanol (PEth) comprises a group of ethanol-derived phospholipids formed from phosphatidylcholine by phospholipase D. The PEth molecular species have a common phosphoethanol head group onto which 2 fatty acid moieties are attached. We developed an electrospray ionization (ESI) LC-MS method for qualitative and quantitative measurement of different PEth species in human blood. Methods: We subjected a total lipid extract of whole blood to HPLC gradient separation on a C4 column and performed LC-ESI-MS analysis using selected ion monitoring of deprotonated molecules for the PEth species and phosphatidylpropanol (internal standard). Identification of individual PEth species was based on ESI–tandem mass spectrometry (MS/MS) analysis of product ions. Results: The fatty acid moieties were the major product ions of PEth, based on comparison with PEth-16:0/16:0, 18:1/18:1, and 16:0/18:1 reference material. For LC-MS analysis of different PEth species in blood, we used a calibration curve covering 0.2–7.0 μmol/L PEth-16:0/18:1. The lower limit of quantitation of the method was <0.1 μmol/L, and intra- and interassay CVs were <9% and <11%. In blood samples collected from 38 alcohol patients, the total PEth concentration ranged between 0.1 and 21.7 μmol/L (mean 8.9). PEth-16:0/18:1 and 16:0/18:2 were the predominant molecular species, accounting for approximately 37% and 25%, respectively, of total PEth. PEth-16:0/20:4 and mixtures of 18:1/18:1 plus 18:0/18:2 (not separated using selected ion monitoring because of identical molecular masses) and 16:0/20:3 plus 18:1/18.2 made up approximately 13%, 12%, and 8%. Conclusions: This LC-MS method allows simultaneous qualitative and quantitative measurement of several PEth molecular species in whole blood samples.

Lipid Classes, Fatty Acid Distributions and Triacylglycerol Molecular Species of Broad Beans (Vicia faba)

2008 ◽  
Vol 85 (6) ◽  
pp. 535-541 ◽  
Author(s):  
Hiromi Yoshida ◽  
Yuka Tomiyama ◽  
Naoko Yoshida ◽  
Masayuki Saiki ◽  
Yoshiyuki Mizushina
Keyword(s):  
Fatty Acid ◽  
Vicia Faba ◽  
Molecular Species ◽  
Lipid Classes ◽  
Broad Beans ◽  
Triacylglycerol Molecular Species

Fatty acid pattern of cerebral lipids in cyanide encephalopathy

1975 ◽  
Vol 11 (5-6) ◽  
pp. 233-238
Author(s):  
M. Wender ◽  
Z. Adamczewska
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Pattern

FATTY ACID PATTERN AND ISCHAEMIC HEART DISEASE

The Lancet ◽  
1987 ◽  
Vol 329 (8543) ◽  
pp. 1202
Author(s):  
C.H. Rapley ◽  
J.B. Ubbink ◽  
L.S. De Villiers
Keyword(s):  
Heart Disease ◽  
Fatty Acid ◽  
Ischaemic Heart Disease ◽  
Fatty Acid Pattern
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