scholarly journals Synergistic and antagonistic interactions of binary mixtures of polycyclic aromatic hydrocarbons in the upregulation of CYP1 activity and mRNA levels in precision-cut rat liver slices

2016 ◽  
Vol 32 (3) ◽  
pp. 764-775 ◽  
Author(s):  
Daphnee S. Pushparajah ◽  
Kathryn E. Plant ◽  
Nick J. Plant ◽  
Costas Ioannides
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Binary Mixtures ◽  
Rat Liver ◽  
Aromatic Hydrocarbons ◽  
Mrna Levels ◽  
Liver Slices ◽  
Polycyclic Aromatic ◽  
Antagonistic Interactions

scholarly journals Interactions between polycyclic aromatic hydrocarbons in binary mixtures: Effects on gene expression and DNA adduct formation in precision-cut rat liver slices

Mutagenesis ◽  
2008 ◽  
Vol 23 (6) ◽  
pp. 491-499 ◽  
Author(s):  
Y. C. M. Staal ◽  
D. S. Pushparajah ◽  
M. H. M. van Herwijnen ◽  
R. W. H. Gottschalk ◽  
L. M. Maas ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polycyclic Aromatic Hydrocarbons ◽  
Binary Mixtures ◽  
Rat Liver ◽  
Aromatic Hydrocarbons ◽  
Adduct Formation ◽  
Dna Adduct ◽  
Liver Slices ◽  
Polycyclic Aromatic

Additive Toxicity of Binary Mixtures of Phototoxic Polycyclic Aromatic Hydrocarbons to the OligochaeteLumbriculus variegatus

1999 ◽  
Vol 154 (1) ◽  
pp. 97-105 ◽  
Author(s):  
Russell J. Erickson ◽  
Gerald T. Ankley ◽  
David L. DeFoe ◽  
Patricia A. Kosian ◽  
Elizabeth A. Makynen
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Binary Mixtures ◽  
Aromatic Hydrocarbons ◽  
Polycyclic Aromatic ◽  
Additive Toxicity

scholarly journals Short-term and Long-term Exposure of the MucilAir™ Model to Polycyclic Aromatic Hydrocarbons

2019 ◽  
Vol 47 (1) ◽  
pp. 9-18 ◽  
Author(s):  
Tereza Cervena ◽  
Kristyna Vrbova ◽  
Andrea Rossnerova ◽  
Jan Topinka ◽  
Pavel Rossner
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Lung Tissue ◽  
Aromatic Hydrocarbons ◽  
Messenger Rna ◽  
Mrna Levels ◽  
Short Term ◽  
Polycyclic Aromatic ◽  
Ldh Release

Cells grown in monocultures are widely used to model lung tissue. As a result of these culture conditions, these cells exhibit poor morphological similarity to those present in in vivo lung tissue. MucilAir™, a 3-D in vitro model comprising human basal, goblet and ciliated cells, represents a fully differentiated respiratory epithelium that can be used as an alternative and a more realistic system. The aim of our study was to compare the effects of short-term and long-term exposure to two polycyclic aromatic hydrocarbons (PAHs) — benzo[a]pyrene (B[a]P) and 3-nitrobenzanthrone (3-NBA) — using MucilAir as a model of human lung tissue. Two concentrations (0.1 μM and 1 μM) were tested at three time points (24 hours, 7 days and 28 days). Several aspects were assessed: cytotoxicity (lactate dehydrogenase (LDH) release), integrity of the cell layer (transepithelial electrical resistance (TEER)), induction of oxidative stress (reactive oxygen species production) and changes in the expression of selected genes involved in PAH metabolism ( CYP1A1 and AKR1C2) and the antioxidant response ( ALDH3A1, SOD1, SOD2, GPX1, CAT, HMOX1 and TXNRD1). The results showed that exposure to B[a]P caused a spike in LDH release at day 5. Exposure to 3-NBA caused a number of spikes in LDH release, starting at day 5, and a decrease in TEER after 11 days. CYP1A1 gene expression was upregulated after the 7-day and 28-day B[a]P exposures, as well as after the 24-hour and 7-day 3-NBA exposures. HMOX1 and SOD1 were downregulated after both 24-hour PAH treatments. HMOX1 was upregulated after a 1-week exposure to 3-NBA. There were no significant changes in the messenger RNA (mRNA) levels of AKR1C2, ALDH3A1, TXNRD1, SOD2, GPX1 or CAT. These results illustrate the potential use of this 3-D in vitro lung tissue model in studying the effects of chronic exposure to PAHs.

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